Antioxidant effects of 14 Chinese traditional medicinal herbs against human low-density lipoprotein oxidation

10.1016/j.jtcme.2014.10.001

Hsin-Hung Lin；Albert Linton Charles；Chang-Wei Hsieh；Ya-Chi Lee；Jhih-Ying Ciou

Chinese medicinal herbs ； low-density lipoprotein ； radical-scavenging activity ； total phenolics ； antioxidant

Journal of Traditional and Complementary Medicine

5卷1期（2015 / 01 / 01）

51 - 55

英文

The relationship between the antioxidant activities and inhibitory effect of 14 Chinese medicinal herbs against oxidized low-density lipoprotein (LDL) formation was evaluated. Prolongation of the lag phase of LDL oxidation depended on the concentration of the herbs. The concentration of each herb that was able to prolong the lag time by about two-fold was calculated and expressed as doubling-time concentration. The lower the doubling-time concentration, the stronger the inhibitory effect exhibited toward LDL oxidation. Among them, Chrysanthemi Flos (Chrysanthemum morifolium ramat; 甘菊花gān jú huā), Crataegi Fructus (Crataegus pinnatifida Bge. var. major N.E.Br.; 山楂shān zhā), and Roselle (Hibiscus sabdariffa Linn.; 洛神luò shén) showed significant inhibitory effects. Correlation coefficients between doubling-time concentration and radical-scavenging activities were high; the total phenolic content was also high. In conclusion, phenolic compounds contributed not only to antioxidant activities, but also to the inhibitory effect against LDL oxidation. Chrysanthemi Flos, Crataegi Fructus, and H. sabdariffa, with lower doubling-time concentrations, could be potent phytochemical agents to reduce LDL oxidation and prevent the progression of atherosclerosis.

1. Lin, PH(2008).The concept of Chinese medicines and foods having the same origin and diets and therapies to treat pain: taking Tangkuei (Angelicae Sinensis Radix) as an example.J Chin Diet Cult,4,53-80.
   連結：
2. Astrup, A(2001).The role of dietary fat in the prevention and treatment of obesity. Efficacy and safety of low-fat diets.Int J Obes Relat Metab Disord,25(Suppl 1),S46-S50.
3. Aviram, M(2000).Review of human studies on oxidative damage and antioxidant protection related to cardiovascular diseases.Free Radic Res,33(Suppl),S85-S97.
4. Cadenas, E,Sies, H(1998).The lag phase.Free Radic Res,28,601-609.
5. Cai, Y,Luo, Q,Sun, M,Corke, H(2004).Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer.Life Sci.,74,2157-2184.
6. Cordova, AC,Jackson, LSM,Berke-Schlessel, DW,Sumpio, BE(2005).The cardiovascular protective effect of red wine.J Am Coll Surg,200,428-439.
7. George, J,Blank, M,Hojnik, M(1997).Oxidized low density lipoprotein (Ox-LDL) but not LDL aggravates the manifestations of experimental antiphospholipid syndrome (APS).Clin Exp Immunol,108,227-233.
8. Gonzalez-Requejo, A,Sanchez-Bayle, M,Baeza, J(1995).Relations between nutrient intake and serum lipid and apolipoprotein levels.J Pediatr,127,53-57.
9. Guilbert, JJ(2003).The World Health Report 2002: REDUCING Risks, Promoting Healthy Life.Abingdon:Education for Health.
10. Hashimoto, R,Yaita, M,Tanaka, K,Hara, Y,Kojo, S(2000).Inhibition of radical reaction of apolipoprotein B-100 and alpha-tocopherol in human plasma by green tea catechins.J Agric Food Chem,48,6380-6383.
11. Hatch, FT(1968).Practical methods for plasma lipoprotein analysis.Adv Lipid Res,6,1-68.
12. Hirunpanich, V,Utaipat, A,Morales, NP(2005).Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat low-density lipoprotein (LDL).Biol Pharm Bull.,28,481-484.
13. Kleinveld, HA,Hak-Lemmers, HL,Stalenhoef, AF,Demacker, PN(1992).Improved measurement of low-density-lipoprotein susceptibility to copper-induced oxidation: application of a short procedure for isolating low-density lipoprotein.Clin Chem,38,2066-2072.
14. Lee, MJ,Chou, FP,Tseng, TH,Hsieh, MH,Lin, MC,Wang, CJ(2002).Hibiscus proto-catechuic acid or esculetin can inhibit oxidative LDL induced by either copper ion or nitric oxide donor.J Agric Food Chem,50,2130-2136.
15. Lee, YC,Chuah, AM,Yamaguchi, T,Takamura, H,Matoba, T(2008).Lee YC, Chuah AM, Yamaguchi T, Takamura H, Matoba T.Food Sci Technol Res,14,205-210.
16. Liu, H,Qiu, N,Ding, H,Yao, R(2008).Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses.Food Res Int,41,363-370.
17. Puhl, H,Waeg, G,Esterbauer, H(1994).Methods to determine oxidation of low-density lipoproteins.Methods Enzymol,233,425-441.
18. Qian, X,Zhang, M(2001).Clinical research on asymptomatic hyperlipemia treated by Da Chai Hu Tang.Forum Tradit Chin Med,16,11-12.
19. Qu, YX(2008).Study on processing technology of natural compound health drink made from hawthorn, jujube, Chinese wolfberry, and chrysanthemum.Food Sci.,29,710-713.
20. Son, S,Lewis, BA(2002).Free radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: structureeactivity relationship.J Agric Food Chem,50,468-472.
21. Steinberg, D,Parthasarathy, S,Carew, TE,Khoo, JC,Witztum, JL(1989).Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity.N Engl J Med.,320,915-924.
22. Stroehla, BC,Malcoe, LH,Velie, EM(2005).Dietary sources of nutrients among rural Native American and white children.J Am Diet Assoc.,105,1908-1916.
23. Takahashi, R,Ohmori, R,Kiyose, C,Momiyama, Y,Ohsuzu, F,Kondo, K(2005).Antioxidant activities of black and yellow soybeans against low density lipoprotein oxidation.J Agric Food Chem,53,4578-4582.
24. Vinson, JA,Su, X,Zubik, L,Bose, P(2001).Phenol antioxidant quantity and quality in foods: fruits.J Agric Food Chem,49,5315-5321.
25. Wu, T-Y,Khor, TO,Saw, CLL,Loh, SC,Chen, AI,Lim, SS(2011).Anti-inflammatory/anti-oxidative stress activities and differential regulation of Nrf2-mediated genes by non-polar fractions of tea Chrysanthemum zawadskii and licorice Glycyrrhiza uralensis.AAPS J.,13,1-13.
26. Wu, YJ,Hong, CY,Lin, SJ,Wu, P,Shiao, MS(1998).Increase of vitamin E content in LDL and reduction of atherosclerosis in cholesterol-fed rabbits by a water-soluble antioxidant-rich fraction of Salvia miltiorrhiza.Arterioscler ThrombVasc Biol,18,481-486.
27. Xiaohong, Y,Jing-Ping, O-Y,Shuzheng, T(2000).Angelica protects the human vascular endothelial cell from the effects of oxidized low-density lipoprotein. in vitro.Clin Hemorheol Microcirc,22,317-323.
28. Yukawa, GS,Mune, M,Otani, H(2004).Effects of coffee consumption on oxidative susceptibility of low-density lipoproteins and serum lipid levels in humans.Biochemistry,69,70-74.
29. Zhang, QH(2009).Homology of medicine and food and Chinese herb taken as food.J Liaoning Univ Tradit Chin Med,11,54-55.
30. Zhang, Z,Chang, Q,Zhu, M,Huang, Y,Ho, WKK,Chen, ZY(2001).Characterization of antioxidants present in hawthorn fruits.J Nutr Biochem,12,144-152.

1. Marcelo Alarcón,Manuel Fuentes,Iván Palomo,Eduardo Fuentes,Cesar Sepúlveda(2018).Buddleja globosa (matico) prevents collagen-induced platelet activation by decreasing phospholipase C-gamma 2 and protein kinase C phosphorylation signaling.Journal of Traditional and Complementary Medicine,8(1),66-71.