Phytochemical, analgesic, antibacterial, and cytotoxic effects of Alpinia nigra (Gaertn.) Burtt leaf extract

10.1016/j.jtcme.2014.11.012

A.M. Abu Ahmed；Farjana Sharmen；Adnan Mannan；Md Atiar Rahman

analgesic ； antibacterial ； cytotoxic ； tail immersion ； Formalin test

Journal of Traditional and Complementary Medicine

5卷4期（2015 / 10 / 01）

248 - 252

英文

This research evaluated the phytochemical contents as well as the analgesic, cytotoxic, and antimicrobial effects of the methanolic extract of Alpinia nigra leaf. Phytochemical analysis was carried out using established methods. The analgesic effects of the extract were measured with the formalin test and tail immersion test. The antibacterial activity of the extract was evaluated using the disc diffusion technique. Cytotoxicity was assessed with the brine shrimp lethality bioassay. Data were analyzed with one-way analysis of variance using statistical software (SPSS, Version 19.0). The qualitative phytochemical screening of A. nigra leaf extract showed the presence of medicinally active secondary metabolites such as alkaloids, glycosides, cardiac glycosides, flavonoids, steroids, tannins, anthraquinone glycosides, and saponins. The extract at a dose of 200 mg/kg revealed a prevailed central nociception increasing the reaction time in response to thermal stimulation. The extract also showed a response to chemical nociceptors, causing pain inhibition in the late phase. The leaf extract (2 mg/disc) showed mild antibacterial activity compared to tetracycline (50 μg/disc). In the brine shrimp lethality bioassay, the LC_(50) (lethal concentration 50) value of the extract was found to be 57.12 μg/mL, implying a promising cytotoxic effect. The results evidenced the moderate analgesic and antibacterial effects with pronounced cytotoxic capability.

1. Apati, P,Houghton, PJ,Kery, A(2004).HPLC investigation of antioxidant components in Solidago herba.Acta Pharm Hung,74,223-231.
2. Biswa Nath Das, BN,Biswas, BK(2012).Anti-inflammatory activity of the rhizome extract of Alpinia nigra.Int Res J Pharm,2,73-76.
3. Calixto, JB(2000).Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents).Braz J Med Biol Res.,33,179-189.
4. Carlisson, KH,Jurna, I(1987).Depression by flupirtine, a novel on analgesic agent of motor and sensory response of nociceptive system in the rat spinal cord.Eur J Pharmacol,143,89-99.
5. Chandrashekar, KS,Satyanarayana, D,Joshi, AB(2002).Analgesic activity of Leucas lavandulaefolia Rees.Indian Drugs,2,78-80.
6. Dubuisson, D,Dennis, SR(1977).The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats.Pain,4,161-174.
7. Fernando, C,Alma, DA(2007).Additional Antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum.Phytother Res,21,78-80.
8. Ghosh, S,Indukuri, K,Bondalapati, S,Saikia, AK,Rangan, L(2013).Unveiling the mode of action of antibacterial labdane diterpenes from Alpinia nigra (Gaertn.) BL Burtt seeds.Eur J Med Chem,66,101-105.
9. Gupta, SS(1994).Prospects and perspectives of natural plants products in medicine.Ind J Pharmacol,26,1-12.
10. Gurib-Fakim, A(2006).Medicinal plants: traditions of yesterday and drugs of tomorrow.Mol Aspects Med,27,1-93.
11. Han, JT,Bang, MH,Chun, OK,Kim, DO,Lee, CY,Baek, NI(2004).Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities.Arch Pharm Res,27,390-395.
12. Haque, M,Obayed Ullah, M,Nahar, K(2009).In vitro antibacterial and cytotoxic activities of different parts of plant Swietenia mahagony.Pak J Biol Sci.,12,599-602.
13. Hunskaar, S,Hole, K(1987).The formalin test in mice: dissociation between inflammatory and non-inflammatory pain.Pain,30,103-114.
14. Iennette, EH(1985).Manual of Clinical Microbiology.Washington, DC:American Association for Microbiology.
15. Janssen, PAJ,Niemegers, CJE,Dony, JGH.(1963).The inhibitory effect of fentanyl and other morphine-like analgesics on the warm water induced tail withdrawal reflex in rats.Azheim Forsch,13,502-507.
16. Karen, AO,Andrea, JD,Paul Sly, WSN,O'Brien, NM,Williamson, G(2001).Flavonoid glucuronides are substrates for human liver L-glucuronidase.FEBS Lett,503,103-106.
17. Kim, HY,Moon, BH,Lee, HJ,Choi, DH(2004).Flavonol glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity.J Ethnopharmacol,93,227-230.
18. Majinda, RR,Motswaledi, M,Waigh, RD,Waterman, PG(1997).Phenolic and antibacterial constituents of Vahlia capensis.Planta Med,63,268-270.
19. Meyer, BN,Ferrigni, NR,Putnam, JE,Jacobsen, LB,Nichols, DE,Mclaughlin, JL(1982).Brine shrimp: a convenient general bioassay for active plant constituents.Planta Med,45,31-34.
20. Ncube, NS,Afolayan, AJ,Okoh, AI(2008).Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends.Afr J Biotechnol,7,1797-1806.
21. Newman, M,Lhuillier, A,Poulsen, AD(2004).Checklist of the Zingiberaceae of Malesia.Blumea,16,23.
22. Panizzi, L,Caponi, C,Catalana, S,Cioni, PL,Morelli, I(2002).In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius.J Ethnopharmacol,79,165-168.
23. Plumb, GW,Price, KR,Williamson, G(1999).Antioxidant properties of flavonol glycosides from green beans.Redox Rep,4,123-127.
24. Prashant, KR,Dolly, J,Singh, KR,Gupta, KR,Watal, G(2008).Glycemic properties of Trichosanthes dioica leaves.Pharm Biol,46,894-899.
25. Qiao, CF,Wang, ZT,Dong, H,LS,Hao, XJ(2000).The chemical constituents of Blackfruit Galangal (Alpinia nigra).Chin Tradit Herbs Drugs,31,404-405.
26. Roy, B,Swargiary, A(2009).Anthelmintic efficacy of ethanolic shoot extract of Alpinia nigra on tegumental enzymes of Fasciolopsis buski, a giant intestinal parasite.J Parasit Dis,33,48-53.
27. Roy, B,Tandon, V.(1999).Flukicidal activity of Alpinia nigra (Zingiberaceae) against the trematode, Fasciolopsis buski in humans.Biomed Lett,60,23-29.
28. Sanchez, C,Gupta, M,Vasquez, M,de Noriega,Montenegro, G(1993).Bioessay with Artemia to predict antibacterial and pharmacologic activity.Rev Med Panama,18,62-69.
29. Sermaani, M(2011).Evaluation of phytochemical and antibacterial activity of Pedalium murex Linn. Root.Int Res J Pharm,2,131-134.
30. Shibata, M,Ohkubo, T,Takahashi, H,Inoki, R(1989).Modified formalin test: characteristic biphasic pain response.Pain,38,347-352.
31. Singab, AN,Youssef, DT,Noaman, E,Kotb, S(2005).Hepatoprotective effect of flavonol glycosides rich fraction from Egyptian Vicia calcarata Desf. against CCl4-induced liver damage in rats.Arch Pharm Res,28,791-798.
32. Sinha, M,Choudhury, MD(2010).Antimicrobial activity of some selected plants from Southern Assam.Assam Univ J Sci Technol,6,58-65.
33. Sofowora, A.(1982).Medicinal Plants and Traditional Medicine in Africa.Chichester, England:John Wiley and Sons.
34. Solis, PN,Wright, CW,Anderson, MM,Gupta, MP,Phillipson, D(1993).A microwell cytotoxicity assay using Artemia salina (Brine Shrimp).Planta Med,59,250-252.
35. Wu, DL(1981).Flora of China.Beijing:Science Press.
36. Wu, ZY,Zhou, TY,Xiao, PG(1988).Jiangsu Institute of Botany. vol. 1.Shanghai:Shanghai Science and Technology Publishing House.

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