Curcumin improves liver damage in male mice exposed to nicotine

10.1016/j.jtcme.2014.11.034

Mohammadreza Salahshoor；Sabah Mohamadian；Seyran Kakabaraei；Shiva Roshankhah；Cyrus Jalili

curcumin ； damage ； liver ； mice ； nicotine

Journal of Traditional and Complementary Medicine

6卷2期（2016 / 04 / 01）

176 - 183

英文

The color of turmeric (薑黃jiāng huáng) is because of a substance called curcumin. It has different pharmacological effects, such as antioxidant and anti-inflammatory properties. Nicotine is a major pharmacologically active substance in cigarette smoke. It is mainly metabolized in the liver and causes devastating effects. This study was designed to evaluate the protective role of curcumin against nicotine on the liver in mice. Forty-eight mice were equally divided into eight groups; control (normal saline), nicotine (2.5 mg/kg), curcumin (10, 30, and 60 mg/kg) and curcumin plus nicotine-treated groups. Curcumin, nicotine, and curcumin plus nicotine (once a day) were intraperitoneally injected for 4 weeks. The liver weight and histology, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and serum nitric oxide levels have been studied. The results indicated that nicotine administration significantly decreased liver weight and increased the mean diameter of hepatocyte, central hepatic vein, liver enzymes level, and blood serum nitric oxide level compared with the saline group (p ＜ 0.05). However, curcumin and curcumin plus nicotine administration substantially increased liver weight and decreased the mean diameter of hepatocyte, central hepatic vein, liver enzymes, and nitric oxide levels in all groups compared with the nicotine group (p ＜ 0.05). Curcumin demonstrated its protective effect against nicotine-induced liver toxicity.

1. Agarwal, A,Nallella, K,Allamaneni, S,Said, T(2008).Role of antioxidants in treatment of male infertility: an overview of the literature.Reprod Biomed Online,8,616-627.
2. Balcerek, M,Matławska, I(2005).Preventive role of curcumin in lung cancer.Przegl Lek,62,1180-1181.
3. Bandyopadhyaya, G,Sinha, S,Chattopadhyay, B,Chakraborty, A(2008).Protective role of curcumin against nicotine-induced genotoxicity on rat liver under restricted dietary protein.Eur J Pharmacol,588,151-157.
4. Basu, S,Haldar, N,Bhattacharya, S,Biswas, S,Biswas, M(2014).Hepatoprotective activity of Litchi chinensis leaf against paracetamol-induced liver damage in rats.Mid East J Sci Res.,20,292-296.
5. Berrahall, A,Hajjaji, N.(2007).Antioxidant enzymes activities and bilirubin level in a adult rats treated with load.C R Biol,330,581-588.
6. Chen, B,Zhu, Z,Chen, M,Dong, W,Li, Z(2014).Three-dimensional quantitative structure-activity relationship study on antioxidant capacity of curcumin analogues.J Mol Struct,1061,134-139.
7. Chigurupati, S,Son, T,Hyun, D,Lathia, J,Mughal, M,Savell, J(2008).Lifelong running reduces oxidative stress and degenerative changes in the testes of mice.J Endocrinol,199,333-341.
8. Chuang, S,Kuo, M,Hs, C(2000).Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis.Carcinogenesis,21,331-335.
9. Cordova, C,Netto, C,Yunes, R(2002).Protective properties of butanolic extract of the Calendula officinalis L. (marigold) against lipid peroxidation of rat liver microsomes and action as free radical scavenger.Redox Rep.,7,95-102.
10. Daniel, S,Limson, J,Dairam, A,Watkins, G,Daya, S(2004).Through metal binding curcumin protects against lead-and cadmiumeinduced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain.J Inorg Biochem,98,266-275.
11. Ebrahimi, S,Sadeghi, H,Pourmahmoudi, A,Askarian, S,Askari, S(2011).Protective effect of zizphus vulgaris extract, on liver toxicity in laboratory rats.Armaghan Danesh,16,172-180.
12. El-Maraghy, SA,El-Sawalhi, MM(2009).Hepatoprotective potential of crocin and curcumin against iron overload-induced biochemical alterations in rat.Afric J Biochem Res,3,215-221.
13. El-Zayadi, A.(2006).Heavy smoking and liver.World J Gastroenterol,12,6098-6101.
14. Fu, Y,Zheng, S,Lin, J,Ryerse, J,Chen, A(2008).Curcumin protects the rat liver from CCl4-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation.Mol Pharmacol,73,399-409.
15. Gawish, AM,Ramadan, S,Hassan, AM,Issa, AM(2010).Morphometrical, histopathological and cytogenetical ameliorating effects of green tea extract on nicotine toxicity of the testis of rats.J Cytol Histol,1,105-110.
16. Ghadami, MR,Pourmotabed, A,Khademi, N(2012).The protective effect of curcumin on scopolamine induced spatial learning and memory deficits in rats.J Kermanshah Univ Med Sci,16,201-210.
17. Hematológico, E(2006).Action of trivalent chromium on rat liver structure. Histometric and haematological studies.Int J Morphol,24,197-203.
18. Hon, WM,Wei, M,Kang Hoe, L,Hoon Eng, K(2002).Nitric oxide in liver diseases.Ann NY Acad Sci,962,275-295.
19. Hukkanen, J,Jacob, P,Benowitz, N(2005).Metabolism and disposition kinetics of nicotine.Pharmacol Rev,57,79-115.
20. Ishita, C,Kaushik, B,Uday, B,Banerjee, R(2004).Turmeric and curcumin biological actions and medicinal applications.Curr Sci,87,44-53.
21. Jalili, C,Salahshoor, MR,Moradi, S,Pourmotabbed, A,Motaghi, M(2014).The therapeutic effect of the aqueous extract of boswellia serrata on the learning deficit in kindled rats.Int J Prev Med,5,563-568.
22. Jalili, C,Salahshoor, MR,Naseri, A(2014).Protective effect of utrica diocia against nicotine-induced damage on reproductive parameters in male mice.Iran J Reprod Med,12,401-408.
23. Jalili, C,Salahshoor, MR,Pourmotabbed, A(2014).The effects of aqueous extract of Boswellia Serrata on hippocampal region CA1 and learning deficit in kindled rats.Res Pharm Sci,9,351-358.
24. Jungil, H,Mousumi, B,Jihyeuny, J,Jae-Ha, R,Xiaoxin, C,Shengmin, S(2004).Modulation of arachidonic acid metabolism by curcumin and related B-diketone derivatives: effect on cytosolic phospholipase A, cyclooxygenases and 5-lipoxygenase.Carcinogenesis,25,1671-1679.
25. Kalpana, C,Sudheer, A,Rajasekharan, K,Menon, V(2007).Comparative effects of curcumin and its synthetic analogue on tissue lipid peroxidation and antioxidant status during nicotine-induced toxicity.Med J.,48,124-130.
26. Khazaei, M,Zarei, M,Sharifi, MR,Pourshanazari, AA(2011).The effect of maintenance and reversal of DOCA-Salt hypertension on extravasation of macromolecules and serum nitric oxide concentration in male rats.Pathophysiology,18,201-206.
27. Khorsandi, L(2008).The protective effect of turmeric (curcuma longa) extract on acetaminophen induced liver damage in mice.Daru,16(3),155-159.
28. Lallemand, F,Dravolina, O,De Witte, P(2006).Nicotine-induced changes of glutamate and arginine in naive and chronically alcoholised rats:an in vivo microdialysis study.Brain Res,1111,48-60.
29. Lari, P,Abnous, K,Imenshahidi, M,Rashedinia, M,Razavi, M,Hosseinzadeh, H(2013).Evaluation of diazinon-induced hepatotoxicity and protective effects of crocin.Toxicol Ind Health,52,218.
30. Li, S,Lu, D,Zhang, Y,Zhang, Y(2014).Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice.J Assist Reprod Genet,31,109-114.
31. Menon, V,Sudheer, A,Kalpana, C.(2007).Antioxidant and anti-inflammatory properties of curcumin.Adv Exp Med Biol,595,105-125.
32. Muller, T,Schepers, G(1997).Evidence for peroxynitrite as an oxidative stress-inducing compound of aqueous cigarette smoke fraction.Carcinogenesis.,18,295-301.
33. Nelson, A,Sidney, D(1990).Molecular mechanisms of the hepatotoxity caused by acetaminophen.Semin Liver Dis,10,267-278.
34. Qureshi, M(2010).In-vitro antioxidant and in-vivo hepatoprotective activity of leucas ciliata leaves.Rec Nat Prod,2,124-130.
35. Reitman, S,Frankel, A(1957).A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase.Am J Clin Pathol,28,53-56.
36. Sadashivam, S,Manickam, A.(1996).Biochemical Methods.India:New Age International Publisher.
37. Sakr, A,Saber, A(2007).Ameliorative effect of ginger (zingiber officinale) on mancozeb fungicide induced liver injury in albino rats.Aust J Basic Appl Sci,1,650-656.
38. Sala, M,Komesu, M,Lopes, R,Maia, G(1994).Karyometric study of basal cell carcinoma.Braz Dental J,5,11-14.
39. Shakhanbeh, J(2001).Teucrium polium inhibits nerve conduction and carrageenan induced inflammation in the rat skin.Turk J Med Sci.,21,15-31.
40. Shen, SQ(2007).Protective effect of curcumin against liver warm ischemia reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes.World J Gastroenterol,13,1953-1961.
41. Suñer, I,Espinosa-Heidmann, G,Marin-Castano, E,Hernandez, P,Pereira-Simon, S,Cousins, S(2004).Nicotine increases size and severity of experimental choroidal neovascularization.Invest Ophthalmol Vis Sci.,45,311-317.
42. Wilken, R(2011).Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma.Mol Cancer,10,4-9.
43. Willis, D,Popovech, M,Gany, F,Hoffman, C,Blum, J,Zelikoff, J(2014).Toxicity of gutkha, a smokeless tobacco product gone global: is there more to the toxicity than nicotine.Int J Environ Res Public Health,11,919-933.
44. Zaitoun, A,Apelqvist, G,Al-Mardini, H,Gray, T,Bengtsson, F,Record, C(2006).Quantitative studies of liver atrophy after portacaval shunt in the rat.J Surg Res.,131,225-232.
45. Zhang, S,Day, I,Ye, S(2001).Nicotine induced changes in gene expression by human coronary artery endothelial cells.Atherosclerosis,154,277-283.
46. Zhao, R,Sharp, BM(2007).Nicotine-induced norepinephrine release in hypothalamic paraventricular nucleus and amygdale is mediated by N-methyl-D-aspartate receptors and nitric oxide in the nucleus tractus solitarius.J Pharmacol Exp Ther,320,837-844.

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