The effects of Nigella sativa on neural damage after pentylenetetrazole induced seizures in rats

10.1016/j.jtcme.2015.06.003

Masoumeh Seghatoleslam；Fatemeh Alipour；Reihaneh Shafieian；Zahra Hassanzadeh；Mohammad Amin Edalatmanesh；Hamid Reza Sadeghnia；Mahmoud Hosseini

Nigella sativa ； Pentylenetetrazole ； Repeated seizures ； Rat ； Neural damage

Journal of Traditional and Complementary Medicine

6卷3期（2016 / 07 / 01）

262 - 268

英文

Nigella sativa (NS) has been suggested to have neuroprotective and anti-seizures properties. The aim of current study was to investigate the effects of NS hydro-alcoholic extract on neural damage after pentylenetetrazole (PTZ) - induced repeated seizures. The rats were divided into five groups: (1) control (saline), (2) PTZ (50 mg/kg, i.p.), (3-5) PTZ-NS 100, PTZ-NS 200 and PTZ-NS 400 (100, 200 and 400 mg/kg of NS extract respectively, 30 min prior to each PTZ injection on 5 consecutive days). The passive avoidance (PA) test was done and the brains were then removed for histological measurements. The PTZNS 100, PTZ-NS 200 and PTZ-NS 400 groups had lower seizure scores than PTZ group (P ＜ 0.01 and P ＜ 0.001). The latency to enter the dark compartment by the animals of PTZ group was lower than control in PA test (P ＜ 0.01). Pre-treatment by 400 mg/kg of the extract increased the latency to enter the dark compartment (P ＜ 0.05). Meanwhile, different doses of the extract inhibited production of dark neurons in different regions of hippocampus (P ＜ 0.001). The present study allows us to suggest that the NS possesses a potential ability to prevent hippocampal neural damage which is accompanied with improving effects on memory.

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