Nephroprotective effect of ethanolic extract of Azima tetracantha root in glycerol induced acute renal failure in Wistar albino rats

10.1016/j.jtcme.2015.05.001

Venugopala Rao Konda；Ruckmani Arunachalam；Madhavi Eerike；Ramesh Rao K；Arun Kumar Radhakrishnan；Lakshmipathy Prabhu Raghuraman；Vinayak Meti；Sobita Devi

Azima tetracantha ； Nephroprotective ； Acute renal failure ； Antioxidant ； Glycerol

Journal of Traditional and Complementary Medicine

6卷4期（2016 / 10 / 01）

347 - 354

英文

The gravity of the impact of renal failure on human health is well known and as there is no specific pharmacotherapy for renal failure, the current study was undertaken to evaluate the effect of root extract of Azima tetracantha, an ancient medicinal plant used in Siddha and Ayurvedhic systems of medicine. The experiment was done in glycerol-induced acute renal failure in Wistar albino rats. Thirty rats were divided into five groups. Group 1 was given normal saline (10 ml/kg) per oral, group 2 with single dose of hypertonic glycerol (8 ml/kg) by intramuscular injection into the hind limbs, group 3 with glycerol and ethanolic extract of A. tetracantha root (ATR) 250 mg/kg, group 4, glycerol and ATR 500 mg/kg and group 5, 500 mg/kg ATR. Extract was given orally 60 min prior to glycerol injection. 24 h urine output, serum creatinine, blood urea nitrogen, total proteins and albumin were measured for all the groups. Kidneys were examined for histopathological changes. The antioxidant activity of the extract was tested in vitro and in vivo. Rats treated with ATR showed significant improvement in biochemical parameters and histopathological changes compared to glycerol treated group. The protective effect was highly significant at 500 mg/kg. Both in vitro and in vivo assays showed significant antioxidant activity. The in vitro activity was comparable to vitamin-C. The ethanolic extract of ATR has nephroprotective effect in glycerol-induced acute renal failure and the mechanism of action could be the antioxidant effect.

1. Abirami, H,Muhammad lyas, MH,Syed Jahangir, H,Prem Kumar, K,Nargis Begum, T(2013).Azima tetracantha: a treasure to be hunted.Int J Recent Sci Res.,4,603-608.
2. Aebi, H(1984).Catalase in vitro.Methods Enzym,105,121-126.
3. Ali, T,Khan, I,Simpson, W(2007).Incidence and outcomes in acute kidney injury: a comprehensive population-based study.J Am Soc Nephrol,18,1292-1298.
4. Antonisamy, P,Duraipandiyan, V,Ignacimuthu, S(2011).Anti-inflammatory, analgesic and antipyretic effects of friedelin isolated from Azima tetracantha Lam. In mouse and rat models.J Pharm Pharmacol.,63,1070-1077.
5. Arthika, S,Shanthammal, Y,SheryIgal, N(2011).Hepatoprotective activity of the ethanolic extract of Azima tetracantha against in paracetamol-induced heap-totoxicity in albino rats.J Adv Pharm Health Care Res,19,14-22.
6. Benavente-García, O,Castillo, J,Marin, F,Ortuño, A,Del Río, J(1997).Uses and properties of Citrus flavonoids.J Agric Food Chem,45,4505-4515.
7. Bennett, R,Mellon, F,Rosa, E,Perkins, L,Kroon, P(2004).Profiling glucosinolates, flavonoids, alkaloids, and other secondary metabolites in tissues of Azima tetracantha L. (Salvadoraceae).J Agric Food Chem.,52,5856-5862.
8. Benzie, I,Strain, J(1996).The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant Power": the FRAP Assay.Anal Biochem,239,70-76.
9. Blois, M(1958).Antioxidant determinations by the use of stable free radical.Nature,181,1199-1200.
10. Burtis, CA(ed.),Ashwood, ER(ed.),Bruns, DE(ed.)(2006).Tietz Textbook of Clinical Chemistry and Molecular Diagnosis.St Louis:Elsevier Saunders.
11. Case, J,Khan, S,Khalid, R,Khan, A(2013).Epidemiology of acute kidney injury in the intensive care unit.Crit Care Res Pract,2013,1-9.
12. Cerdá, J,Bagga, A,Kher, V,Chakravarthi, R(2008).The contrasting characteristics of acute kidney injury in developed and developing countries.Nat Clin Pract Nephrol,4,138-153.
13. Chander, V,Chopra, K(2005).Molsidomine, a nitric oxide donor and l-arginine protects against rhabdomyolysis-induced myoglobinuric acute renal failure.Biochimica et Biophysica Acta (BBA) - General Subjects,1723,208-214.
14. Chander, V,Chopra, K(2006).Protective effect of resveratrol, a polyphenolic phytoalexin on glycerol-induced acute renal failure in rat kidney.Ren Fail,28,161-169.
15. Curry, S,Chang, D,Connor, D(1989).Drug- and toxin-induced rhabdomyolysis.Ann Emerg Med.,18,1068-1084.
16. De Jesus Soares, T,Volpini, R,Francescato, H,Costa, R,da Silva, C,Coimbra, T(2007).Effects of resveratrol on glycerol-induced renal injury.Life Sci.,81,647-656.
17. Duraipandiyan, V,Gnanasekar, M,Ignacimuthu, S(2010).Antifungal activity of triterpenoid isolated from Azima tetracantha leaves.Folia Histochema Cytobiol,48,311-313.
18. Ellman, G(1959).Tissue sulfhydryl groups.Archives Biochem Biophysics,82,70-77.
19. Fang, Y,Ding, X,Zhong, Y(2010).Acute kidney injury in a Chinese hospitalized population.Blood Purif,30,120-126.
20. Ftouh, S,Thomas, M(2013).Acute kidney injury guideline development group. Acute kidney injury: summary of NICE guidance.Br Med J.,347,f4930.
21. Gayathri, G,Nair Bindu, R,Babu, V(2012).GC-MS Analysis of Azima tetracantha.J Pharm Res,5,3746-3747.
22. Gowrisri, M,Kotagiri, Sarita,Vrushabendra Swamy, BM,Archana Swamy, P,Vishwanath, KM(2012).Anti-oxidant and nephroprotective activities of Cassia occidentalis leaf extract against paracetamol induced nephrotoxicity in rats.Res J Pharm Biol Chem Sci.,3,572-586.
23. Green, L,Wagner, D,Glogowski, J,Skipper, P,Wishnok, J,Tannenbaum, S(1982).Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids.Anal Biochem,126,131-138.
24. Gupta, MB,Nath, R,Srivastava, N(1996).Anti-inflammatory and antipyretic activities of beta sitosterol.Int J Immunopharmacol,18,693-700.
25. Hepsibha, Thendral,Sathiya, S,Saravana Babu, C,Premalakshmi, V,Sekarstidied, T(2010).In vitro studies on antioxidant and free radical scavenging activities of Azima tetracantha Lam leaf extracts.Indian J Sci Technol,3,5.
26. Hiremath, VT,Taranath, TC(2010).Traditional phytotherapy for snake bites by tribes of Chitradurga district, Karnataka, India.Ethnobot Leafl,14,120-125.
27. Hsu, C,McCulloch, C,Fan, D,Ordonez, J,Chertow, G,Go, A(2007).Community-based incidence of acute renal failure.Kidney Int,72,208-212.
28. Ismail, T,Gopalakrishnan, S,Begum, V,Elango, V(1997).Anti-inflammatory activity of Salacia oblonga Wall and Azima tetracantha Lam.J Ethnopharmacol,56,145-152.
29. Karam, H,Bruneval, P,Clozel, JP,Löffler, BM,Bariéty, J,Clozel, M(1995).Role of endothelin in acute renal failure due to rhabdomyolysis in rats.J Pharmacol Exp Ther,274,481-486.
30. Kaufman, J,Dhakal, M,Patel, B,Hamburger, R(1991).Community-acquired acute renal failure.Am J Kidney Dis,17,191-198.
31. Khwaja, A(2012).KDIGO clinical practice guidelines for acute kidney injury.Nephron Clin Pract,120,179-184.
32. Kirtikar, KR,Basu, BD(2001).Indian Medicinal Plants.Deharadun:Oriental Enterprises.
33. Lafrance, J,Miller, D(2010).Acute kidney injury associates with increased long-term mortality.J Am Soc Nephrol,21,345-352.
34. Legssyer, A,Ziyyat, A,Mekh, H(2004).Tannins and catechin gallate mediate the vasorelaxant effect of Arbutus unedo on the rat isolated aorta.Phytother Res,18,889-894.
35. Longo, DL(ed.),Fauci, AS(ed.),Kasper, DL(ed.),Hauser, SL(ed.),Jameson, JL(ed.),Loscalzo, J(ed.)(2005).Harrison's Principles of Internal Medicine.New York, NY:McGraw-Hill.
36. Marklund, S,Marklund, G(1974).Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.Eur J Biochem,47,469-474.
37. Moron, M,Depierre, J,Mannervik, B(1979).Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver.Biochimica et Biophysica Acta (BBA) - General Subjects,582,67-78.
38. Murugan, R,Kellum, J(2011).Acute kidney injury: what's the prognosis?.Nat Rev Nephrol,7,209-217.
39. Nargis Begum, T,Muhammad Ilyas, MH,Kalavathy, S,VijayaAnand, A,Sampath Kumar, P,Senthil, R(2009).Effects of ethanolic leaf extract of Azima tetracantha Lam. on ehrlich ascites carcinoma tumour bearing mice.Res J Med Sci,4,351-354.
40. Olagunjua, JA,Adeneyeb, AA,Fagbohunkac, BS(2009).Nephroprotective activities of the aqueous seed extract of Carica papaya Linn. in carbon tetrachloride induced renal injured Wistar rats: a dose- and time-dependent study.Biol Med,1,11-19.
41. Pydi, Ramya,Rajalakshmi, I,Indumathy, S,Kavimani, S(2011).Nephroprotective medicinal plants - a review.Int J Univers Pharm Life Sci.,1,266-281.
42. Rice-Evans, CA(ed.),Packer, L(ed.)(1998).Flavonoids in Health and Disease.New York:Marcel Dekker Inc.
43. Rotruck, J,Pope, A,Ganther, H,Swanson, A,Hafeman, D,Hoekstra, W(1973).Selenium: biochemical role as a component of glutathione peroxidase.Science,179,588-590.
44. Ruch, R,Cheng, S,Klaunig, J.(1989).Prevention of cytotoxicity and inhibition of inter-cellular communication by antioxidant catechins isolated from Chinese green tea.Carcinogenesis,10,1003-1008.
45. Schrier, R,Wang, W,Poole, B,Mitra, A(2004).Acute renal failure: definitions, diagnosis, pathogenesis, and therapy.J Clin Invest,114,5-14.
46. Singh, D,Chander, V,Chopra, K(2004).Protective effect of naringin, a bioflavonoid on glycerol-induced acute renal failure in rat kidney.Toxicology,201,143-151.
47. Sunil, C,Duraipandiyan, V,Ignacimuthu, S,Al-Dhabi, N(2013).Antioxidant, free radical scavenging and liver protective effects of friedelin isolated from Azima tetracantha Lam. leaves.Food Chem,139,860-865.
48. Talele Bharti, D,Mahajan Raghunath, T,Chopda Manojkumar, Z,Nemade Namrata, V(2012).Nephroprotective plants: a review.Int J Pharm Pharm Sci,1,8-16.
49. Uchino, S,Bellomo, R,Morimatsu, H(2007).Continuous renal replacement therapy: a worldwide practice survey.Intensive Care Med.,33,1563-1570.
50. Vanholder, R,Sever, MS,Erek, E,Lameire, N(2000).Rhabdomyolysis.J Am Soc Nephrol,11,1553-1561.
51. Wolfert, A,Oken, D(1989).Glomerular hemodynamics in established glycerol-induced acute renal failure in the rat.J Clin Invest,84,1967-1973.
52. Zurovsky, Y.(1993).Models of glycerol-induced acute renal failure in rats.J Basic Clin Physiology Pharmacol,4,213-218.