Curcumin reverses neurochemical, histological and immuno-histochemical alterations in the model of global brain ischemia

10.1016/j.jtcme.2015.10.001

Glaura Fernandes Teixeira de Alcântara；Eudes Simões-Neto；Giovany Michely Pinto da Cruz；Maria Elizabeth Pereira Nobre；Kelly Rose Tavares Neves；Geanne Matos de Andrade；Gerly Anne de Castro Brito；Glauce Socorro de Barros Viana

Curcumin ； Brain ischemia ； Neuroprotection ； Neuroinflammation ； Cytokines

Journal of Traditional and Complementary Medicine

7卷1期（2017 / 01 / 01）

14 - 23

英文

Curcumin, a curcuminoid from Curcuma longa, presents antioxidant and anti-inflammatory actions and, among pathological changes of cerebral ischemic injury, inflammation is an important one. The objectives were to study the neuroprotective action of curcumin, in a model of global ischemia. Male Wistar rats (sham-operated, ischemic untreated and ischemic treated with curcumin, 25 or 50 mg/kg, p.o.) were anesthesized and their carotid arteries occluded, for 30 min. The SO group had the same procedure, except for carotid occlusion. In the 1^(st) protocol, animals were treated 1 h before ischemia and 24 h later; and in the 2^(nd) protocol, treatments began 1 h before ischemia, continuing for 7 days. Twenty four hours after the last administration, animals were euthanized and measurements for striatal monoamines were performed, at the 1^(st) and 7^(th) days after ischemia, as well as histological and immunohistochemical assays in hippocampi. We showed in both protocols, depletions of DA and its metabolites (DOPAC and HVA), in the ischemic group, but these effects were reversed by curcumin. Additionally, a decrease seen in 5-HT contents, 1 day after ischemia, was also reversed by curcumin. This reversion was not seen 7 days later. On the other hand, a decrease observed in NE levels, at the 7^(th) day, was totally reversed by curcumin. Furthermore, curcumin treatments increased neuronal viability and attenuated the immunoreactivity for COX-2 and TNF-alpha, in the hippocampus in both protocols. We showed that curcumin exerts neuroprotective actions, in a model of brain ischemia that are probably related to its anti-inflammatory activity.

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