Evaluation of analgesic and anti-inflammatory activity of Bridelia retusa (Spreng) bark

10.1016/j.jtcme.2016.12.009

Anil U. Tatiya；Ajay K. Saluja；Mohan G. Kalaskar；Sanjay J. Surana；Prakash H. Patil

Bridelia retusa Spreng ； Anti-inflammatory ； CFA ； Euphorbiaceae ； Protein denaturation ； Leukocyte migration

Journal of Traditional and Complementary Medicine

7卷4期（2017 / 10 / 01）

441 - 451

英文

Several species of Bridelia have been used in the condition of pain & arthritis in Indian folk medicine. Present study revealed the preliminary phytochemical investigation and evaluation of analgesic, anti-inflammatory and anti-arthritic activity as well as underlying mechanism of bark of Bridelia retusa Spreng. (Euphorbiaceae). The bark was subjected to extraction using pet.ether, ethyl acetate and acetone. All the extracts were significantly inhibit abdominal writhings response and licking time in late phase of formalin test. Extracts could also significantly inhibit mean paw edema of rats induced by carrageenan & histamine at dose of 200 & 400 mg/kg, i.p. Test materials also showed significant dose dependent reduction in cotton pellet granuloma & acetic acid induced vascular permeability at 400 mg/kg. Oral administration of B. retusa fractions in CFA induced arthritic rats, physical, biochemical and hematological parameters observed in arthritic animals were altered significantly to near normal condition. The maximum paw edema inhibition at day 21 was observed at 400 mg/kg. It also proved significant protection against protein denaturation & RBC membrane damage. The GC-MS analysis of EA extract revealed the presence of β-sitosterol, stigmasterol, lupeol and friedelin (Pentacyclic triterpenoid). Therefore present study has demonstrated the analgesic; anti-inflammatory and anti-arthritic activities of B. retusa bark and suggested that the molecular membrane might be associated with inhibition of biochemical and hematological parameters. Overall bioactive profile of B. retusa used phytomedicine in future for inflammatory conditions.

1. (1981).Textbook of Rheumatology.Philadelphia:WB Saunders.
2. Amresh, G,Singh, P,Rao, CV(2007).Antinociceptive and antiarthritic activity of Cissampelos pareira roots.J Ethnopharmacol,111(3),531-536.
3. Anonymous(1988).The Wealth of India: Raw Materials: 2(B).New Delhi:CSIR, Publication and Information Directorate.
4. Antonio, M,Brito, AS(1998).Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turn-eraceae).J Ethnopharmacol,61(3),215-228.
5. Bahorun, T,Trotin, F,Pommery, J,Vasseur, J,Pinkas, M(1994).Antioxidant activities of Crataegus monogyna extracts.Planta Med,60(04),323-328.
6. Barbier, A,Breliere, J,Remandet, B,Roncucci, R(1986).Studies on the chronic phase of adjuvant arthritis: effect of SR 41319, a new diphosphonate.Ann Rheum Dis,45(1),67-74.
7. Basu, BD,Kirtikar, K.(1999).Indian Medicinal Plants.International Book Distributors.
8. Bendele, A(2001).Animal models of rheumatoid arthritis.J Musculoskelet Neuronal Interact,1(4),377-385.
9. Bose, A,Mondal, S,Gupta, JK,Ghosh, T,Dash, GK,Si, S(2007).Analgesic, anti-inflammatory and antipyretic activities of the ethanolic extract and its fractions of Cleome rutidosperma.Fitoterapia,78(7),515-520.
10. Bouic, PJ(2002).Sterols and sterolins: new drugs for the immune system?.Drug Discov Today,7(14),775-778.
11. Butler, SH,Godefroy, F,Besson, J-M,Weil-Fugazza, J(1992).A limited arthritic model for chronic pain studies in the rat.Pain,48(1),73-81.
12. Cai, X,Wong, Y,Zhou, H(2006).The comparative study of SpragueeDawley and Lewis rats in adjuvant-induced arthritis.Naunyn Schmiedeberg Arch Pharmacol,373(2),140-147.
13. Calixto, JB,Cabrini, DA,Ferreira, J,Campos, MM(2000).Kinins in pain and inflammation.Pain,87(1),1-5.
14. Chatterjee, S,Das, S(1996).Anti-arthritic and anti-inflammatory effect of a poly-herbal drug (EASE): its mechanism of action.Indian J Pharmacol,28(2),116.
15. Chattopadhyay, P,Besra, S,Gomes, A(2004).Anti-inflammatory activity of tea (Camellia sinensis) root extract.Life Sci,74(15),1839-1849.
16. Chopra, RN,Nayar, S.(1992).Glossary of Indian Medicinal Plants.New Delhi:CSIR, Publication and Information Directorate.
17. Cimmino, M,Accardo, S(1990).Changes in the isoenzyme pattern of alkaline phosphatase in patients with rheumatoid arthritis.Clin Chem,36(7),1376-1377.
18. Cimmino, M,Buffrini, L,Barisone, G,Bruzzone, M,Accardo, S(1989).Alkaline phosphatase activity in the serum of patients with rheumatoid arthritis.Z Rheumatol,49(3),143-146.
19. Collier, H,Dinneen, L,Johnson, CA,Schneider, C(1968).Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse.Br J Pharmacol Chemother,32(2),295-310.
20. Dharmasiri, M,Jayakody, J,Galhena, G,Liyanage, S,Ratnasooriya, W(2003).Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo.J Ethnopharmacol,87(2),199-206.
21. Di Rosa, M,Giroud, J,Willoughby, D(1971).Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine.J Pathol,104(1),15-29.
22. Franzotti, E,Santos, C,Rodrigues, H,Mourao, R,Antoniolli, A(2000).Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malvabranca).J Ethnopharmacol,72(1),273-277.
23. Gartlehner, G,Hansen, RA,Jonas, BL,Thieda, P,Lohr, KN(2006).The comparative efficacy and safety of biologics for the treatment of rheumatoid arthritis: a systematic review and metaanalysis.J Rheumatology,33(12),2398-2408.
24. Gokhale, A,Damre, A,Kulkarni, K,Saraf, M(2002).Preliminary evaluation of anti-inflammatory and anti-arthritic activity of S. lappa, A. speciosa and A. aspera.Phytomedicine,9(5),433-437.
25. Gupta, M,Nath, R,Srivastava, N,Shanker, K,Kishor, K,Bhargava, K(1980).Anti-inflammatory and antipyretic activities of b-sitosterol.Planta Med,39(06),157-163.
26. Gupta, O,Sharma, N,Chand, D(1992).A sensitive and relevant model for evaluating anti-inflammatory activity-papaya latex-induced rat paw inflammation.J Pharmacol Toxicol Methods,28(1),15-19.
27. Hossain, M,Alam, M,Chowhdury, N(2011).Antioxidant, analgesic and anti-inflammatory activities of the herb Eclipta prostrata.J Pharmacol Toxicol,6(5),468-480.
28. Hunskaar, S,Fasmer, OB,Hole, K(1985).Formalin test in mice, a useful technique for evaluating mild analgesics.J Neurosci Methods,14(1),69-76.
29. Klinkhoff, A(2004).Biological agents for rheumatoid arthritis.Drugs,64(12),1267-1283.
30. Koster, R,Anderson, M,De Beer, E(1959).Acetic Acid-induced Analgesic Screening.Federation Proceedings,18
31. Ma, H-Y,Kou,J-P,Wang, J-R,Yu, B-Y(2007).Evaluation of the anti-inflammatory and analgesic activities of Liu-Shen-Wan and its individual fractions.J Ethnopharmacol,112(1),108-114.
32. Maldini, M,Sosa, S,Montoro, P(2009).Screening of the topical anti-inflammatory activity of the bark of Acacia cornigera Willdenow, Byrsonima crassifolia Kunth, Sweetia panamensis Yakovlev and the leaves of Sphagneticola trilobata Hitchcock.J Ethnopharmacol,122(3),430-433.
33. Mehare, ID,Hatapakki, BC(2003).Anti-inflammatory activity of bark of Bridelia retusa Spreng.Indian J Pharm Sci.,65(4),410-411.
34. Mythilypriya, R,Shanthi, P,Sachdanandam, P.(2008).Salubrious effect of Kalpaamruthaa, a modified indigenous preparation in adjuvant-induced arthritis in ratsda biochemical approach.Chem-Biol Interact.,173(2),148-158.
35. Newbould, B(1963).Chemotherapy of arthritis induced in rats by mycobacterial adjuvant.Br J Pharmacol Chemother,21(1),127-136.
36. Okoli, C,Akah, P,Nwafor, S,Anisiobi, A,Ibegbunam, I,Erojikwe, O(2007).Anti-inflammatory activity of hexane leaf extract of Aspilia africana CD Adams.J Ethnopharmacol,109(2),219-225.
37. Oladosu, I,Ogundajo, A,Alyalaagbe, O,Emenyonu, N(2011).Phytochemical and anti-tuberculosis activity of Coffea brivipes, hiern exracts.Res J Phytochem,5,130-135.
38. Price, C,Sammons, H(1974).The nature of the serum alkaline phosphatases in liver diseases.J Clin Pathol.,27(5),392-398.
39. Rall, L,Roubenoff, R.(2004).Rheumatoid cachexia: metabolic abnormalities, mechanisms and interventions.Rheumatology,43(10),1219-1223.
40. Rang, HP,Ritter, JM,Flower, RJ,Henderson, G(2014).Rang & Dale's Pharmacology: With Student Consult Online Access.Elsevier Health Sciences.
41. Rani, HS,Madhavi, G,Srikanth, B,Jharna, P,Rao, U,Jyothy, A(2006).Serum ADA and Creactive protein in rheumatoid arthritis.Int J Hum Genet,6(3),195.
42. Rosalki, S,Foo, A,Tanner, P(1982).Serum gamma-glutamyltransferase and alkaline phosphatase in rheumatoid arthritis.J Clin Pathol,35(12),1395.
43. Roubenoff, R,Freeman, LM,Smith, DE,Abad, LW,Dinarello, CA,Kehayias, JJ(1997).Adjuvant arthritis as a model of inflammatory cachexia.Arthritis Rheum,40(3),534-539.
44. Shinde, U,Phadke, A,Nair, A,Mungantiwar, A,Dikshit, V,Saraf, M(1999).Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil.Fitoterapia,70(3),251-257.
45. Somasundaram, S,Sadique, J,Subramoniam, A(1983).In vitro absorption of [14 C] leucine during inflammation and the effect of antiinflammatory drugs in the jejunum of rats.Biochem Med,29(2),259-264.
46. Suba, V,Murugesan, T,Kumaravelrajan, R,Mandal, SC,Saha, B(2005).Antiinflammatory, analgesic and antiperoxidative efficacy of Barleria lupulina Lindl. extract.Phytother Res,19(8),695-699.
47. Sulaiman, MR,Hussain, M,Zakaria, ZA(2008).Evaluation of the antinociceptive activity of Ficus deltoidea aqueous extract.Fitoterapia,79(7),557-561.
48. Tjølsen, A,Berge, O-G,Hunskaar, S,Rosland, JH,Hole, K(1992).The formalin test: an evaluation of the method.Pain,51(1),5-17.
49. Trease, G,Evans, W(1983).Drugs of Biological Origin.United Kingdom:Balliere Tindall.
50. Tripathy, S,Sahoo, S,Pradhan, D,Sahoo, S,Satapathy, D.(2009).Evaluation of anti arthritic potential of Hybanthus enneaspermus.Afr J Pharm Pharmacol.,3(12),611-614.
51. Vasudevan, M,Gunnam, KK,Parle, M(2007).Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract.J Ethnopharmacol,109(2),264-270.
52. Wang, Q,Kuang, H,Su, Y(2013).Naturally derived anti-inflammatory compounds from Chinese medicinal plants.J Ethnopharmacol,146(1),9-39.
53. Whittle, B(1964).The use of changes in capillary permeability in mice to distinguish between narcotic and nonnarcotic analgesics.Br J Pharmacol Chemother.,22(2),246-253.
54. Winter, CA,Porter, CC(1957).Effect of alterations in side chain upon anti-inflammatory and liver glycogen activities of hydrocortisone esters.J Am Pharm Assoc.,46(9),515-519.
55. Yokota, J,Takuma, D,Hamada, A(2006).Scavenging of reactive oxygen species by Eriobotrya japonica seed extract.Biol Pharm Bull,29(3),467-471.
56. Yoshizaki, K,Nishimoto, N,Mihara, M,Kishimoto, T(1998).Therapy of rheumatoid arthritis by blocking IL-6 signal transduction with a humanized anti-IL-6 receptor antibody.Springer seminars in immunopathology

1. Wisuwat Thongphichai,Surattana Amnuoypol,Suchada Sukrong,Rutt Suttisri,Peththa Wadu Dasuni Wasana,Pasarapa Towiwat,Hasriadi,Anusara Jongchanapong(2023).Antinociceptive efficacy of Clerodendrum petasites S. Moore, a Thai medicinal plant, and its CNS safety profiles.Journal of Traditional and Complementary Medicine,13(1),81-92.