Protective effect of hydro-alcoholic extract of Salvia haematodes Wall root on cognitive functions in scopolamine-induced amnesia in rats

10.1016/j.jtcme.2016.12.008

Mohammad Shawwal；Badruddeen；Mohammad Khushtar；Md. Azizur Rahman

Amnesia ； Alzheimer's disease ； Cognition ； Rivastigmine ； Salvia haematodes ； Scopolamine

Journal of Traditional and Complementary Medicine

7卷4期（2017 / 10 / 01）

471 - 475

英文

Diminished cholinergic transmission may be responsible for development of amnesia. Hence, the present study was undertaken to investigate the possible protective effect of hydro-alcoholic extract of Salvia haematodes Wall root (HESH) on cognitive functions in scopolamine-induced amnesia in adult Sprague Dawley rats. The rats were divided randomly into five groups each consisting of five rats (n = 5). Rats of the groups I, II, III, IV, and V received orally normal saline (10 ml/kg b. wt.), normal saline (10 ml/kg), standard drug rivastigmine (1.5 mg/kg), HESH (20 mg/kg), and HESH (40 mg/kg), respectively once a day for fourteen days. Then, they were subjected to single dose of scopolamine (1 mg/kg b. wt. ip) except in group I on fourteenth day 60 min after respective normal saline or drug administration. They were observed for the effects on step down latency (SDL), locomotor activity and brain AChE activity for the learning and memory. The acquisition SDL, retention SDL and locomotor activity were significantly (p ＜ 0.01) decreased while AChE activity was significantly (p ＜ 0.01) increased in scopolamine-treated group II as compared to normal control group I. The acquisition SDL, retention SDL and locomotor activity were significantly (p ＜ 0.01) increased while, AChE activity was significantly (p ＜ 0.01) decreased with all the doses of HESH and in rivastigmine-treated group as compared to scopolamine-treated group II. Hydro-alcoholic extract of S haematodes root possesses protective effect on cognitive functions and may prove to be a useful memory restorative agent in the management of cognitive dysfunctions as in amnesia and Alzheimer's diseases.

1. Hamidpour, M,Hamidpour, R,Hamidpour, S,Shahlari, M(2014).Chemistry, Pharmacology, and medicinal property of Sage (Salvia) to prevent and cure illnesses such as obesity, diabetes, depression, dementia, lupus, autism, heart disease, and cancer.J Tradit Complement Med,4(2),82-88.
   連結：
2. Abdille, MH,Singh, RP,Jayaprakasha, GK,Jena, BS(2005).Antioxidant activity of the extracts from Dillenia indica fruits.Food Chem,90(4),891-896.
3. Adam, JN,Sivropoulov, A,Kokkini, S,Lanaras, T,Arsenakis, M(1998).Antifungal activities of Origarnum vulgare subsp. Hirtum, Mentah spicata, Lavandula angustifolia and Salvia fruticose essential oils against human pathogenic fungi.J Agric Food Chem,46,1739-1745.
4. Ageel, AM,Mossa, JS,Tariq, M,Al-Yahya, MA,Al-Saeed, MS(1987).Plants used in Saudi folk medicine.Riyadh, Saudi Arabia:King Saud University.
5. Akbar, A,Tariq, M,Nisa, M(1985).Pharmacological studies on Salvia haematodes Wall.Acta Trop,42(4),371-374.
6. Anonyms(2013).Diagnostic and Statistical Manual of Mental Disorders, 5th Edition: DSM-5.Washington, D.C:American Psychiatric Association.
7. Avicena, 1048. AI-Qanoon-fil-rib (vol. 2, Urdu translation). Nawal Kishore, Lucknow, India; p. 52.
8. Badruddeen, Fareed S,Siddiqui, HH,Haque, SE(2012).Psychoimmunomodulatory activity of Salvadora persica L. (Miswak) extract on stress model in rats.Asian J Trad Med,7(3),109-117.
9. Badruddeen,Fareed, S,Siddiqui, HH,Haque, SE,Khalid, M,Akhtar, J(2012).Effects of Onosma bracteatum Wall. (Gaozaban) on stress model in Sprague Dawley rats.J Clin Diagn Res.,6(suppl 7),1356-1360.
10. Baitar I. Jame-ul-Mufradar-al-Advia-wal-Agh:ia. Egypt: Amara Press; 1871:121.
11. Baylac, S,Racine, P(2003).Inhibition of 5-lopoxygenase by essential oils and other natural fragrant extracts.Int J Aromather,13,138-142.
12. Bejar, C,Wang, R,Weinstock, M(1999).Effect of rivastigmine on scopolamine-induced memory impairment in rats.Eur J Pharmacol,383,231-240.
13. Bordilau, R,Spiridon, L,Teaca, CA(2009).Anti-inflammatory constituents from different medicinal plants.JCIV Eng Manag,8(4),785-792.
14. Bowen, DM,Smith, CB,White, P,Dawson, AN(1976).Neurotransmitter-related enzymes and indices of hypoxia in senile dementia and other abiotrophies.Brain,99,459-496.
15. Dymock, W,Warden, CH,Hooper, D(1972).Pharmacographia Indicavol.Karachi, Pakistan:Institute of Health and Tibbi Research, Hamdard National Foundation.
16. Ellman, GL,Courtney, KD,Anders, F,Featherstone, RM(1961).A new and rapid colori-metric determination of acetylcholinesterase activity.Biochem Pharmacol,7,88-95.
17. Kabir, H(2003).Shamsher's Morakkabat (Unani Formulations).Mirza Aligarh, India:Shamsher Publisher and Distributors.
18. Khan, AQ(1980).Khan AQ. Personal Communication. 1980..
19. Khan MA. Muheet-e-Azam I. Kanpur, India: Matba Nizami; 1886:378.
20. Kulkarni, KS,Kasture, SB,Mengi, SA(2010).Efficacy study of Prunus amygdalus (almond) nuts in scopolamine-induced amnesia in rats.Indian J Pharmacol,42(3),168-173.
21. Lowry, OH,Rosenbrough, NJ,Forr, AL,Randall, RJ.(1951).Protein measurement with the Folin's phenol reagent.J Biol Chem,193,265-275.
22. Nadkarni, AK(1954).Indian Materia Medica.Bombay, India:Dhootapapeshwar Prakashan Ltd.
23. Quinette, P,Guillery-Girard, B,Dayan, J(2006).What does transient global amnesia really mean? Review of the literature and thorough study of 142 cases.Brain,129(Part 7),1640-1658.
24. Rahman, MA,Kamal, M,Hussain, A(2015).Phytochemical and analytical evaluation of Kachnal (Bauhinia racemosa Lam.) Bark.Curr Trad Med,1,136-144.
25. Raja, R,Jeeva, S,Prakash, J,Marimuthu, J,Irudayaraj, V(2011).Antibacterial activity of selected ethnomedicinal plants from South India.Asian Pac J Trop Med,4(5),375-378.
26. Said, M(1969).Pharmacopoeia of Eastern Medicine.Karachi, Pakistan:The Times Press.
27. Stevens, R(1981).Scopolamine impairs spatial maze performance in rats.Physion Behav,27,385-386.
28. Usmani, A,Mujahid, M,Khushtar, M,Siddiqui, HH,Rahman, MA(2016).Hepatoprotective effect of Anacyclus pyrethrum Linn against antitubercular drug-induced hepatotoxicity in SD rats.J Complement Integr Med,13(3),295-300.
29. Whitehouse, PJ,Price, DL,Clark, AW,Coyle, JT,DeLong, MR(1982).Alzheimer disease: evidence for selective loss of cholinergic neurons in the nucleus basalis.Ann Neurol,10,122-126.