Phenolic constituents and modulatory effects of Raffia palm leaf (Raphia hookeri) extract on carbohydrate hydrolyzing enzymes linked to type-2 diabetes

10.1016/j.jtcme.2017.01.003

Felix A. Dada；Sunday I. Oyeleye；Opeyemi B. Ogunsuyi；Tosin A. Olasehinde；Stephen A. Adefegha；Ganiyu Oboh；Aline A. Boligon

Raffia palm leaf ； Type-2 diabetes ； Antioxidant ； Phenolic compounds ； Anti-hyperglycemia

Journal of Traditional and Complementary Medicine

7卷4期（2017 / 10 / 01）

494 - 500

英文

This study sought to investigate the effects of Raffia palm (Raphia hookeri) leaf extract on enzymes linked to type-2 diabetes mellitus (T2DM) and pro-oxidant induced oxidative stress in rat pancreas. The extract was prepared and its α-amylase and α-glucosidase inhibitory effects were determined. Radical [2,2- diphenyl-1-picrylhydrazyl (DPPH)] scavenging and Fe^(2+)-chelating abilities, and inhibition of Fe^(2+)-induced lipid peroxidation in rat pancreas homogenate were assessed. Furthermore, total phenol and flavonoid contents, reducing property, and high performance liquid chromatography diode array detector (HPLC-DAD) fingerprint of the extract were also determined. Our results revealed that the extract inhibited α-amylase (IC_(50) = 110.4 μg/mL) and α-glucosidase (IC_(50) = 99.96 μg/mL) activities in concentration dependent manners which were lower to the effect of acarbose (amylase: IC_(50) = 18.30 μg/mL; glucosidase: IC_(50) = 20.31 μg/mL). The extract also scavenged DPPH radical, chelated Fe^(2+) and inhibited Fe^(2+)-induced lipid peroxidation in rat pancreas all in concentration dependent manners with IC_(50) values of 402.9 μg/mL, 108.9 μg/mL and 367.0 μg/mL respectively. The total phenol and flavonoid contents were 39.73 mg GAE/g and 21.88 mg QAE/g respectively, while the reducing property was 25.62 mg AAE/g. The HPLC analysis revealed the presence of chlorogenic acid (4.17 mg/g) and rutin (5.11 mg/g) as the major phenolic compounds in the extract. Therefore, the ability of the extract to inhibit carbohydrate hydrolyzing enzymes and protect against pancreatic oxidative damage may be an important mechanisms supporting its antidiabetic properties and could make Raffia palm leaf useful in complementary/alternative therapy for management of T2DM. However, further studies such as in vivo should be carried out.

1. Ademiluyi, AO,Oboh, G,Aragbaye, F,Oyeleye, SI,Ogunsuyi, OB(2015).Antioxidant properties and in vitro a-amylase and a-glucosidase inhibitory properties of phenolics constituents from different varieties of Corchorus spp in rat pancreas.J Taibah Univ Med Sci,10(3),278-287.
2. Ahmed, OM,Monei, AA,Yazid, IA,Mahmoud, AM(2010).Antihyperglycemic, anti-hyperlipidemic and antioxidant effects and the probable mechanisms of action of Ruta graveolens infusion and rutin in nicotinamide-streptozotocin-induced diabetic rats.Diabetol Croat,39-41.
3. Akpan, EJ,Usoh, IF(2004).Phytochemical screening and effect of aqueous root extract of Raphia hookeri (Raffia palm) on metabolic clearance rate of ethanol in rabbits.Biokemistri,16,37-42.
4. Alausa, SK,Oyesiku, OO,Aderibigbe, JO,Akinola, OS(2011).Thermal properties of Calamus deërratus, Raphia hookeri and synthetic board in building design in Southwestern Nigeria.Afr J Plant Sci,5(4),281-283.
5. Apostolidis, E,Kwon, YI,Shetty, K(2007).Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension.Innov Food Sci Emerg Tech,8,46-54.
6. Arts, ICW,Hollman, PCH(2005).Polyphenols and disease risk in epidemiologic studies.Am J Clin Nutr.,81,317-325.
7. Bayır, H,Kochanek, PM,Kagan, VE(2006).Oxidative stress in immature brain after traumatic brain injury.Dev Neurosci,28,420-431.
8. Belle, EM,Duret, L,Galtier, N,Eyre-Walker, A(2004).The declines of isochors in mammals, an assessment of the GC content variation along the mammalian phylogeny.J Mol Evol.,58,653-660.
9. Bischoff, H(1994).Pharmacology of alpha-glucosidase inhibition.Eur J Clin Investig,24,3-10.
10. Chu, Y,Sun, J,Wu, X,Liu, J(2002).Antioxidant and antiproliferative activity of common vegetables.J Agric Food Chem,50,6910-6916.
11. Clarke, G,Ting, KN,Wiart, C,Fry, J(2013).High correlation of 2,2-diphenyl-1-picrylhy-drazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest.Antioxidants,2,1-10.
12. Cosentino, F,Hishikawa, H,Katusic, ZS,Luscher, TF(1997).High glucose increases nitric oxide synthase expression and superoxide anion generation in human aortic endothelial cells.Circulation,96,25-28.
13. Dudonné, S,Vitrac, X,Coutière, P,Woillez, M,Mérillon, JM(2009).Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays.J Agric Food Chem,57,1768-1774.
14. Gouni-Berthold, I,Krone, W(2006).Diabetic ketoacidosis and hyperosmolar hyper-glycemic state.Med Klin,1,100-105.
15. Graf, BA,Milbury, PE,Blumberg, JB(2005).Flavonols, flavonones, flavanones and human health: epidemological evidence.J Med Food,8,281-290.
16. Gyamfi, MA,Yonamine, M,Aniya, Y(1999).Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally induced liver injuries.General Pharm,32,661-667.
17. Janbaz, KH,Saeed, SA,Gilani, AH(2002).Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents.Fitoterapia,73,557-563.
18. Kim, JW,Yoon, K.(2011).Glucolipotoxicity in pancreatic b-cells.Diabetes Metab J.,35,444-450.
19. Kwon, YI,Apostolidis, E,Kim, YC,Shetty, K(2007).Health benefits of traditional corn, beans and pumpkin: in vitro studies for hyperglycemia and hypertension management.J Med Food.,10,266-275.
20. Lambrinoudaki, I,Tsouvalas, E,Vakaki, M(1997).Osteoprotegerin, soluble receptor activator of nuclear factor-κB ligand, and subclinical atherosclerosis in children and adolescents with type 1 diabetes mellitus.Int J Endocrinol,8
21. Lenzen, S(2008).Oxidative stress: the vulnerable b-cell.Biochem Soc Trans.,36,343-347.
22. Masuo, Kazuko(ed.)(2003).Type 2 Diabetes.
23. Matsuda, H,Morikawa, T,Yoshikawa, M(2002).Antidiabetogenic constituents from several natural medicines.Pure Appl Chem,74(7),1301-1308.
24. Mbaka, GO,Ogbonnia, SO,Banjo, AE(2012).Activity of Raphia hookeri root extract on blood glucose, lipid profile and glycosylated haemoglobin on alloxan induced diabetic rats.J Morphol Sci,29(3),214-222.
25. Mbaka, GO,Ogbonnia, SO,Oyeniran, KJ,Awopetu, PI(2012).Effect of Raphia hookeri seed extract on blood glucose, glycosylated haemoglobin and lipid profile of alloxan induced diabetic rats.Br J Med Medic Res,2(4),621.
26. Meda, A,Lamie, CE,Romito, M,Millogo, J,Nacoulma, GO(2005).Determination total phenolic, flavonoid and proline content in Burkina Faso honeyens as well as their radical scavenging activity.Food Chem,91,571-577.
27. Minotti, G,Aust, SD(1987).An investigation into the mechanism of citrate-Fe2+-dependent lipid peroxidation.Free Radic Bio Med.,3,379-387.
28. Noctor, G,De, Paepe, R,Foyer, CH(2007).Mitochondrial redox biology and homeostasis in plants.Trends Plant Sci,12,125-134.
29. Oboh, G,Agunloye, OM,Adefegha, SA,Akinyemi, AJ,Ademiluyi, AO(2015).Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study.J Basic Clin Physiol Pharmacol,26(2),165-170.
30. Oboh, G,Akomolafe, TL,Adefegha, SA,Adetuyi, AO(2011).Inhibition of cyclophosphamide induced oxidative stress in brain by polar and non-polar extracts of Annatto (Bixa orellana) seeds.Exp Toxicol Path,63,257-262.
31. Oboh, G,Ogunsuyi, OB,Ogunbadejo, MD,Adefegha, SA(2016).Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose.J Food Drug Anal.
32. Oboh, G,Olasehinde, TA,Ademosun, AO(2014).Essential oil from lemon peels inhibits key enzymes linked to neurodegenerative conditions and pro-oxidant induced lipid peroxidation.J Oleo Sci.,63,4373-4381.
33. Ohkawa, H,Ohishi, N,Yagi, K(1979).Assay for lipid peroxidase in animal tissues by thiobarbitaric acid reaction.Anal Biochem,95,351.
34. Oyaizu, M.(1986).Studies on products of browning reaction: antioxidative activity of products ofbrowning reactionprepared from glucosamine.JpnJNutri,44,307-315.
35. Puntel, RL,Nogueira, CW,Rocha, JBT(2005).Krebs cycle intermediates modulate thio-barbituric acid reactive species (TBARS) production in rat brain in vitro.Neurochem Res,78,225-235.
36. Qader, SW,Abdulla, MA,Chua, LS,Najim, N,Zain, NN,Hamdan, S(2011).Antioxidant, total phenolic content and cytotoxicity evaluation of selected Malaysian plants.Molecules,16,3433-3443.
37. Raalte, DHV,Diamant, M(2011).Glucolipotoxicity and beta cells in type 2 diabetes mellitus: target for durable therapy.Diabetes Res Clin Pract,93(1),37-46.
38. Rajmohan, L,Deepa, R,Mohan, A,Mohan, V(2000).Association between isolated hypercholesterolemia, isolated hypertriglyceridemia and coronary artery disease in south Indian type 2 diabetic patients.Indian Heart J.,52,400-406.
39. Sakai, K,Matsumoto, K,Nishikawa, T(2003).Mitochondrial reactive oxygen species reduce insulin secretion by pancreatic beta-cells.Biochem Biophysics Res Comm,300,216-222.
40. Saliu, JA,Ademiluyi, AO,Akinyemi, AJ,Oboh, G.(2012).In vitro antidiabetes and anti-hypertension properties of phenolic extracts from bitter leaf (Vernonia amygdalina Del.).J Food Biochem,36(5),569-576.
41. Shah, SV,Fonseca, VA(2011).Iron and diabetes revisited.Diabetes Care,34,1676-1677.
42. Shodehinde, SA,Adefegha, SA,Oboh, G(2016).Phenolic composition and evaluation of methanol and aqueous extracts of bitter Gourd (Momordica charantia L) leaves on angiotensin-I-converting enzyme and some pro-oxidant-induced lipid peroxidation in vitro.J Evid Based Complementary Altern Med,21,NP67-NP76.
43. Singleton, VL,Orthofor, R,Lamuela-Raventos, RM(1999).Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent.Methods Enzym,299,152-178.
44. Thilagam, E,Parimaladevi, B,Kumarappan, C,Mandal, SC(2013).a-glucosidase and aamylase inhibitory activity of Senna surattensis.J Acupunct Meridian Stud.,6,24-30.
45. Worthington, V(ed.)(1993).Worthington Enzyme Manual.Freehold, NJ:Worthington Biochemical Corp.
46. Yang, H,Li, X(2012).The role of fatty acid metabolism and lipotoxicity in pancreatic beta-cell injury: identification of potential therapeutic targets.Acta Pharm Sin B.,2(4),396-402.