Traditional Persian Medicine and management of metabolic dysfunction in polycystic ovary syndrome

10.1016/j.jtcme.2017.04.006

Ayda Hosseinkhani；Nasrin Asadi；Mehdi Pasalar；Mohammad M. Zarshenas

Traditional Persian Medicine ； Polycystic ovary syndrome ； Metabolic dysfunction ； Medicinal plant ； Pharmacopoeia ； Persia

Journal of Traditional and Complementary Medicine

8卷1期（2018 / 01 / 01）

17 - 23

英文

Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age. Its cause is unknown and it remains the most enigmatic of reproductive disorders. The extant written documents of Traditional Persian Medicine (TPM) - with holistic approaches towards human health - contain remedies used for centuries. Before further experimental research on any of these treatments, it is appropriate to study current related scientific evidence on their possible pharmacological actions. This work aims to study PCOS and its treatments in TPM. To collect data from medieval medicinal texts, six of the most famous manuscripts of Persian medicine were studied. Medicinal treatments for a problem similar to PCOS were searched for in these books. The plants were listed and their authentications were confirmed in accordance with botanical books. PubMed and ScienceDirect databases were searched for related mechanisms of action or pharmacological activities of the medicinal plants reported. From numerous articles, the current work tried to cite the latest publications with regard to each reported plant and PCOS-related mechanisms of action. We studied herbal treatments recommended by ancient Persians to treat a condition called Habs-e-tams, which had the same symptoms of PCOS. It could be concluded that ancient physicians not only wanted to treat the irregular menstrual cycle-which is the most obvious symptom of PCOS-but also their treatment options were aimed at ameliorating the related underlying metabolic dysfunctions. The recommended herbs, which have the most scientific proof for their related actions, can be studied further in experimental analyses.

1. Ahmad, N,Rahman, Z,Akhtar, N,Ali, S(2012).Effects of aqueous methanolic extract of flax seeds (Linum usitatissimum) on serum estradiol, progesterone, kidney and liver functions and some serum biochemical metabolites in immature female rats.Pak Vet J.,32,211-215.
2. Ahmed, OM,Moneim, AA,Yazid, IA,Mahmoud, AM(2010).Antihyperglycemic, anti-hyperlipidemic and antioxidant effects and the probable mechanisms of action of Ruta graveolens infusion and rutin in nicotinamide-streptozotocin-induced diabetic rats.Diabetol Croat,39,15-35.
3. Althnaian, T(2014).Influence of dietary supplementation of Garden cress (Lepidium sativum L.) on liver histopathology and serum biochemistry in rats fed high cholesterol diet.J Adv Vet Animal Res.,1,216-223.
4. Arentz, S,Abbott, JA,Smith, CA,Bensoussan, A(2014).Herbal medicine for the management of polycystic ovary syndrome (PCOS) and associated oligo/amenorrhoea and hyperandrogenism; a review of the laboratory evidence for effects with corroborative clinical findings.BMC Complement Altern Med,14,511.
5. Arokiyaraj, S,Balamurugan, R,Augustian, P(2011).Antihyperglycemic effect of Hypericum perforatum ethyl acetate extract on streptozotocin-induced diabetic rats.Asian Pac J Trop Biomed,1,386-390.
6. Bao, L,Bai, S,Borijihan, G.(2012).Hypolipidemic effects of a new piperine derivative GB-N from Piper longum in high-fat diet-fed rats.Pharm Biol,50,962-967.
7. Belguith-Hadriche, O,Ammar, S,del Mar Contreras, M(2016).Antihyperlipidemic and antioxidant activities of edible Tunisian Ficus carica L. Fruits in high fat diet-induced hyperlipidemic rats.Plant Foods Hum Nutr,71,183-189.
8. Bettaieb, A,Hosein, E,Chahed, S(2015).Decreased adiposity and enhanced glucose tolerance in shikonin treated mice.Obesity,23,2269-2277.
9. Bnouham, M,Merhfour, F-Z,Ziyyat, A,Mekhfi, H,Aziz, M,Legssyer, A(2003).Anti-hyperglycemic activity of the aqueous extract of Urtica dioica.Fitoterapia,74,677-681.
10. Bolkent, S,Yanardag, R,Karabulut-Bulan, O,Yesilyaprak, B(2005).Protective role of Melissa officinalis L. extract on liver of hyperlipidemic rats: a morphological and biochemical study.J Ethnopharmacol.,99,391-398.
11. Celik, O,Acbay, O(2012).Effects of metformin plus rosuvastatin on hyperandrogenism in polycystic ovary syndrome patients with hyperlipidemia and impaired glucose tolerance.J Endocrinol Investig,35,905-910.
12. Cemek, M,Kağa, S,Şimşek, N,Büyükokuroğlu, ME,Konuk, M(2008).Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats.J Nat Med.,62,284-293.
13. Chashti, A(2004).Exir-e Azam (19th century).Tehran:Research Institute for Islamic and Complementary Medicine.
14. Chauhan, K,Sharma, S,Agarwal, N,Chauhan, S,Chauhan, B(2012).A study on potential hypoglycemic and hypolipidemic effects of Lepidium Sativum (Garden Cress) in alloxan induced diabetic rats.Am J PharmTech Res.,2,522-535.
15. Chevassus, H,Gaillard, JB,Farret, A(2010).A fenugreek seed extract selectively reduces spontaneous fat intake in overweight subjects.Eur J Clin Pharmacol,66,449-455.
16. Chung, MJ,Cho, S-Y,Bhuiyan, MJH,Kim, KH,Lee, S-J.(2010).Anti-diabetic effects of lemon balm (Melissa officinalis) essential oil on glucose-and lipid-regulating enzymes in type 2 diabetic mice.Br J Nutr.,104,180-188.
17. Costantini, S,Colonna, G,Castello, G(2014).A holistic approach to study the effects of natural antioxidants on inflammation and liver cancer.Cancer Treat Res,159,311-323.
18. Crosignani, PG,Colombo, M,Vegetti, W,Somigliana, E,Gessati, A,Ragni, G(2003).Overweight and obese anovulatory patients with polycystic ovaries: parallel improvements in anthropometric indices, ovarian physiology and fertility rate induced by diet.Hum Reprod,18,1928-1932.
19. Das, M,Sarma, B,Rokeya, B(2011).Antihyperglycemic and antihyperlipidemic activity of Urtica dioica on type 2 diabetic model rats.J Diabetol,2,1-6.
20. Eddouks, M,Lemhadri, A,Michel, JB(2004).Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats.J Ethnopharmacol,94,143-148.
21. Eddouks, M,Lemhadri, A,Michel, J-B(2005).Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats.J Ethnopharmacol,98,345-350.
22. Eidi, A,Eidi, M,Esmaeili, E(2006).Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats.Phytomedicine,13,624-629.
23. Ekoh, SN,Akubugwo, EI,Ude, VC,Edwin, N(2014).Anti-hyperglycemic and anti-hyperlipidemic effect of spices (Thymus vulgaris, Murraya koenigii, Ocimum gratissimum and Piper guineense) in alloxan-induced diabetic rats.Int J Biosci,4,179-187.
24. Elahi, A,Fereidooni, A,Shahabinezhad, F,Tafti, MA,Zarshenas, M-M(2016).An over-view of amenorrhea and respective remedies in Traditional Persian Medicine.Trends Pharma Sci,2(1),3-10.
25. El-Desoky, GE,Aboul-Soud, MA,Al-Numair, KS(2012).Antidiabetic and hypolipidemic effects of Ceylon cinnamon (Cinnamomum verum) in alloxan-diabetic rats.J Med Plant Res.,6,1685-1691.
26. El-Soud, N,El-Laithy, N,El-Saeed, G(2011).Antidiabetic activities of Foeniculum vulgare mill. Essential oil in streptozotocin-induced diabetic rats.Maced J Med Sci.,4,139-146.
27. Fabricant, DS,Farnsworth, NR(2001).The value of plants used in traditional medicine for drug discovery.Environ Health Perspect,1,69-75.
28. Fararh, KM,Atoji, Y,Shimizu, Y,Shiina, T,Nikami, H,Takewaki, T(2004).Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters.Res Vet Sci.,77,123-129.
29. Farideh, ZZ,Bagher, M,Ashraf, A,Akram, A,Kazem, M(2010).Effects of chamomile extract on biochemical and clinical parameters in a rat model of polycystic ovary syndrome.J Reprod Infertil,11,169-174.
30. Farkhad, NK,Farokhi, F,Tukmacki, A(2012).Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats.Avicenna J Phytomed.,2,179.
31. Fauser, BC,Tarlatzis, BC,Rebar, RW(2012).Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-sponsored 3rd PCOS Consensus Workshop Group.Fertil Steril,97,28-38.
32. Fontanari, GG,Batistuti, JP,Cruz, RJD,Saldiva, PHN,Arêas, JAG(2012).Cholesterol-lowering effect of whole lupin (Lupinus albus) seed and its protein isolate.Food Chem,132,1521-1526.
33. Gambineri, A,Pelusi, C,Vicennati, V,Pagotto, U,Pasquali, R(2002).Obesity and the polycystic ovary syndrome.Int J Obes Relat Metab Disord.,26,883-896.
34. Ghahraman, A,Okhovvat, A.(2004).Matching the Old Medicinal Plant Names with Scientific Terminology.Tehran:Tehran University Press.
35. Ghasemzadeh, A,Farzadi, L,Khaki, A,Khan Ahmadi, S(2013).Effect of Allium cepa seeds ethanolic extract on experimental polycystic ovary syndrome (PCOS) apoptosis induced by estradiol-valerate.Life Sci J.,10,170-175.
36. Ghule, AE,Jadhav, SS,Bodhankar, SL(2012).Effect of ethanolic extract of seeds of Linum usitatissimum (Linn.) in hyperglycaemia associated ROS production in PBMNCs and pancreatic tissue of alloxan induced diabetic rats.Asian Pac J Trop Dis,2,405-410.
37. Gorji, A(2003).Pharmacological treatment of headache using Traditional Persian Medicine.Trends Pharm Sci.,24,331-334.
38. Hafizur, RM,Kabir, N,Chishti, S(2012).Asparagus officinalis extract controls blood glucose by improving insulin secretion and β-cell function in streptozotocin-induced type 2 diabetic rats.Br J Nutr.,108,1586-1595.
39. Haj-Husein, I,Tukan, S,Alkazaleh, F(2016).The effect of marjoram (Origanum majorana) tea on the hormonal profile of women with polycystic ovary syndrome: a randomised controlled pilot study.J Hum Nutr Diet,29,105-111.
40. Hannan, JMA,Rokeya, B,Faruque, O(2003).Effect of soluble dietary fibre fraction of Trigonella foenum graecum on glycemic, insulinemic, lipidemic and platelet aggregation status of Type 2 diabetic model rats.J Ethnopharmacol,88,73-77.
41. Helal, EG,Eid, FA,Wahsh, AM,Ahmed, E(2011).Effect of fennel (Foeniculum vulgare) on hyperlipidemic rats.Egypt J Hosp Med,43,212-226.
42. Helal, EG,Mahmoud, A,El-Badawy, EE,Kahwash, AA(2005).Effect of Commiphora myrrha extract on some physiological parameters and histological changes in diabetic albino rats.Egypt J Hosp Med,148-162.
43. Husain, GM,Chatterjee, SS,Singh, PN,Kumar, V(2011).Hypolipidemic and antiobesity-like activity of standardised extract of Hypericum perforatum L. in rats.ISRN Pharmacol,2011,505247.
44. Huseini, HF,Darvishzadeh, F,Heshmat, R,Jafariazar, Z,Raza, M,Larijani, B(2009).The clinical investigation of Citrullus colocynthis (L.) schrad fruit in treatment of Type II diabetic patients: a randomized, double blind, placebo-controlled clinical trial.Phytother Res,23,1186-1189.
45. Ibn Sina=Avicenna(1998).Kitāb al-Qānūn fīal-Tibb.New Delhi:Senior Press Superintendent, Jamia Hamdard Printing Press.
46. Jashni, HK,Jahromi, HK,Ranjbary, AG,Jahromi, ZK,Kherameh, ZK(2016).Effects of aqueous extract from Asparagus officinalis L. roots on hypothalamic-pituitary-gonadal axis hormone levels and the number of ovarian follicles in adult rats.Int J Reprod Biomed,14,75.
47. Javed, I,Zia-Ur-Rahman, N,Khan, MZ(2009).Antihyperlipidaemic efficacy of Trachyspermum ammi in albino rabbits.Acta Vet Brno,78,229-236.
48. Jia, S,Hu, Y,Zhang, W(2015).Hypoglycemic and hypolipidemic effects of neo-hesperidin derived from Citrus aurantium L. in diabetic KK-A(y) mice.Food Funct,6,878-886.
49. Kassem, A,Al-Aghbari, A,Molham, A-H,Al-Mamary, M(2006).Evaluation of the potential antifertility effect of fenugreek seeds in male and female rabbits.Contraception.,73,301-306.
50. Kazemipoor, M,Radzi, CW,Hajifaraji, M,Haerian, BS,Mosaddegh, MH,Cordell, GA(2013).Antiobesity effect of caraway extract on overweight and obese women: a randomized, triple-blind, placebo-controlled clinical trial.Evid Based Complement Altern Med,2013,928582.
51. Khalesi, S,Jamaluddin, R,Ismail, A(2011).Effect of raw and heated flaxseed (Linum usitatissimum L.) on blood lipid profiles in rats.Int J Appl Sci Tech,1,84-89.
52. Khare, CP(2007).Indian Medicinal Plants.New York:Springer.
53. Khorasani, A(2008).Moalejat (18th century).Tehran:Research Institute for Islamic and Complementary Medicine.
54. Kim, HU,Ryu, JY,Lee, JO,Lee, SY(2015).A systems approach to traditional oriental medicine.Nat Biotech,33,264-268.
55. Le, MT,Lanaspa, MA,Cicerchi, CM(2016).Bioactivity-guided identification of botanical inhibitors of ketohexokinase.PLoS One,11,e0157458.
56. Lee, J,Chae, K,Ha, J(2008).Regulation of obesity and lipid disorders by herbal extracts from Morus alba, Melissa officinalis, and Artemisia capillaris in high-fat diet-induced obese mice.J Ethnopharmacol,115,263-270.
57. Legro, RS,Arslanian, SA,Ehrmann, DA(2013).Diagnosis and treatment of polycystic ovary syndrome: an Endocrine Society clinical practice guideline.J Clin Endocrinol Metabol,98,4565-4592.
58. Legro, RS,Kunselman, AR,Dodson, WC,Dunaif, A(1999).Prevalence and predictors of risk for type 2 diabetes mellitus and impaired glucose tolerance in polycystic ovary syndrome: a prospective, controlled study in 254 affected women.J Clin Endocrinol Metabol,84,165-169.
59. Lemhadri, A,Hajji, L,Michel, J-B,Eddouks, M.(2006).Cholesterol and triglycerides lowering activities of caraway fruits in normal and streptozotocin diabetic rats.J Ethnopharmacol,106,321-326.
60. Leonti, M(2013).Traditional medicines and globalization: current and future perspectives in ethnopharmacology.Front Pharmacol,4,92.
61. Malik, ZA,Sharma, PL(2011).An ethanolic extract from licorice (glycyrrhiza glabra) exhibits anti-obesity effects by decreasing dietary fat absorption in a high fat diet-induced obesity rat model.Int J Pharm Sci Res,2,3010.
62. Mansi, K,Abushoffa, AM,Disi, A,Aburjai, T(2009).Hypolipidemic effects of seed extract of celery (Apium graveolens) in rats.Pharmacogn Mag,5,301.
63. Mansour, HA,Newairy, A-SA,Yousef, MI,Sheweita, SA(2002).Biochemical study on the effects of some Egyptian herbs in alloxan-induced diabetic rats.Toxicology,170,221-228.
64. Messinis, IE,Messini, CI,Anifandis, G,Dafopoulos, K(2015).Polycystic ovaries and obesity.Best Pract Res Clin Obstet Gynaecol,29,479-488.
65. Mirhosseini, M,Baradaran, A,Rafeian-Kopaei, M(2014).Anethum graveolens and hyperlipidemia: a randomized clinical trial.J Res Med Sci.,19,758-761.
66. Mohammadi, A,Gholamhoseinian, A,Fallah, H(2014).Zataria multiflora increases insulin sensitivity and PPARgamma gene expression in high fructose fed insulin resistant rats.Iran J Basic Med Sci,17,263-270.
67. Mokaberinejad, R,Zafarghandi, N,Bioos, S(2012).Mentha longifolia syrup in secondary amenorrhea: a double-blind, placebo-controlled, randomized trials.DARU J Pharm Sci.,20,1.
68. Monsefi, M,Ghasemi, M,Bahaoddini, A(2006).The effects of Anethum graveolens L. on female reproductive system of rats.DARU J Pharm Sci.,14,131-135.
69. Mozaffarian, V(2006).Dictionary of Iranian Plant Names.Tehran:Farhang Moaser Press.
70. Noratto, GD,Garcia-Mazcorro, JF,Markel, M(2014).Carbohydrate-free peach (Prunus persica) and plum (Prunus domestica) juice affects fecal microbial ecology in an obese animal model.PLoS One,9,e101723.
71. Ozsoy-Sacan, O,Yanardag, R,Orak, H,Ozgey, Y,Yarat, A,Tunali, T(2006).Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin-induced diabetic rats.J Ethnopharmacol,104,175-181.
72. Panda, S(2008).The effect of Anethum graveolens L. (dill) on corticosteroid induced diabetes mellitus: involvement of thyroid hormones.Phytother Res,22,1695-1697.
73. Pari, L,Venkateswaran, S(2004).Protective role of Phaseolus vulgaris on changes in the fatty acid composition in experimental diabetes.J Med Food,7,204-209.
74. Patil, SJ,Patil, SB(2008).Estrogenic activity of petroleum ether extract of seeds of Citrus medica on immature albino rats.Int J Green Pharm,2,91-94.
75. Peixoto, JS,Comar, JF,Moreira, CT(2012).Effects of Citrus aurantium (bitter orange) fruit extracts and p-synephrine on metabolic fluxes in the rat liver.Molecules,17(5),5854-5869.
76. Perez Gutierrez, RM(2012).Inhibition of advanced glycation end-product formation by Origanum majorana L. in vitro and in streptozotocin-induced diabetic rats.Evid Based Complement Altern Med,2012
77. Perez, C,Dominguez, E,Canal, J,Campillo, J,Torres, M(2000).Hypoglycaemic activity of an aqueous extract from Ficus carica (fig tree) leaves in streptozotocin diabetic rats.Pharm Biol,38,181-186.
78. Pintana, H,Sripetchwandee, J,Supakul, L,Apaijai, N,Chattipakorn, N,Chattipakorn, S(2014).Garlic extract attenuates brain mitochondrial dysfunction and cognitive deficit in obese-insulin resistant rats.Appl Physiol Nutr Metabol,39,1373-1379.
79. Pourghassem-Gargari, B,Ebrahimzadeh-Attary, V,Rafraf, M,Gorbani, A(2009).Effect of dietary supplementation with Nigella sativa L. on serum lipid profile, lipid peroxidation and antioxidant defense system in hyperlipidemic rabbits.J Med Plant Res.,3,815-821.
80. Ramesh, B,Karuna, R,Reddy, SS,Sudhakara, G,Saralakumari, D(2013).Ethanolic extract of Commiphora mukul gum resin attenuates streptozotocin-induced alterations in carbohydrate and lipid metabolism in rats.EXCLI J.,12,556.
81. Ranjan, V,Vats, M,Gupta, N,Sardana, S(2014).Antidiabetic potential of the whole plant of Adiantum capillus veneris Linn. in streptozotocin-induced diabetic rats.Int J Pharm Chem Res,6,341-347.
82. Rhazes(2005).Kitāb al-hāwī fīal-Tibb.Tehran:Academy of Medical Sciences.
83. Sadeghpour, N,Khaki, AA,Najafpour, A,Dolatkhah, H,Montaseri, A(2015).Study of Foeniculum vulgare (Fennel) seed extract effects on serum level of estrogen, progesterone and prolactin in mouse. A general policy.Crescent J Med Biol Sci.,2,59-63.
84. Saini, S,Sharma, S(2013).Antidiabetic effect of Helianthus annuus L., seeds ethanolic extract in streptozotocinnicotinamide induced type 2 diabetes mellitus.Int J Pharm Pharm Sci.,5,382-387.
85. Sharma, MS,Choudhary, PR(2016).Effect of fenugreek seeds powder (Trigonella foenum-graecum L.) on experimental induced hyperlipidemia in rabbits.J Diet Suppl.,12,1-8.
86. Shepherd, J,Hull Royal Infirmary Hull U(2016).Insulin resistance and oxidative stress in obese PCOS, nonobese PCOS and controls.Insulin,1,357.
87. Shirazi, A(2009).Makhzan al-adviyah.Tehran:Tehran University of Medical Sciences.
88. Shobha, R,Rajeshwari, C,Andallu, B.(2013).Anti-peroxidative and anti-diabetic activities of aniseeds (Pimpinella anisum L.) and identification of bioactive compounds.Am J Phytomed Clin Ther,1,516-527.
89. Singh, RB,Niaz, MA,Ghosh, S.(1994).Hypolipidemic and antioxidant effects of Commiphora mukul as an adjunct to dietary therapy in patients with hypercho-lesterolemia.Cardiovasc Drugs Ther,8,659-664.
90. Soliman, HA,Eltablawy, NA,Hamed, MS(2015).The ameliorative effect of Petroselinum crispum (parsley) on some diabetes complications.J Med Plants,3,92-100.
91. Soltani, A(2004).Dictionary of Medicinal Plants.Tehran:Arjmand Press.
92. Sozen, I,Arici, A(2000).Hyperinsulinism and its interaction with hyperandrogenism in polycystic ovary syndrome.Obstet Gynecol Surv.,55,321-328.
93. Tansaz, M,Bahmani, M(2016).Principles of nutrition in patients with polycystic ovary syndrome in Iranian traditional medicine and comparison with modern medicine.Iran J Med Sci.,41,S49.
94. Toserkani, A,Jalali, MR,Najafzaheh, H(2012).Changes of lipid profiles, glucose, and hemogram after administration of Ruta graveolens extract in diabetic rats.Comp Clin Path.,21,1587-1592.
95. Tunakabuni, D(2007).Tuhfat al-mu'minin.Tehran:Research Center of Traditional Medicine, Shahid Beheshti University of Medical Sciences.
96. Vasanthkumar, R,Jeevitha, M.(2014).Evaluation of antiobesity activity of Apium graveolens stems in rats.Int J Chem Pharm Sci.,5,159-163.
97. Venkateswaran, S,Pari, L,Saravanan, G(2002).Effect of Phaseolus vulgaris on circulatory antioxidants and lipids in rats with streptozotocin-induced diabetes.J Med Food.,5,97-103.
98. Visavadiya, NP,Narasimhacharya, AV(2006).Hypocholesterolaemic and antioxidant effects of Glycyrrhiza glabra (Linn) in rats.Mol Nutr Food Res,50,1080-1086.
99. Vrbikova, J(2012).Polycystic ovary syndrome: why there is no cure.Expert Rev Endocrinol Metab,7,475-477.
100. Vrbikova, J,Cibula, D.(2005).Combined oral contraceptives in the treatment of polycystic ovary syndrome.Hum Reprod Update,11,277-291.
101. Yang, Y,Zhou, L,Gu, Y(2007).Dietary chickpeas reverse visceral adiposity, dyslipidaemia and insulin resistance in rats induced by a chronic high-fat diet.Br J Nutr,98,720-726.
102. Yoshinari, O,Shiojima, Y,Igarashi, K(2012).Anti-obesity effects of onion extract in Zucker diabetic fatty rats.Nutrients,4,1518-1526.
103. Zhu, X,Zhang, W,Zhao, J,Wang, J,Qu, W(2010).Hypolipidaemic and hepatoprotective effects of ethanolic and aqueous extracts from Asparagus officinalis L. by-products in mice fed a high-fat diet.J Sci Food Agric,90,1129-1135.
104. Zhu, Z,Jiang, W,Thompson, HJ(2012).Edible dry bean consumption (Phaseolus vulgaris L.) modulates cardiovascular risk factors and diet-induced obesity in rats and mice.Br J Nutr,108,S66-S73.

1. Madhavi Patel,Disha P. Prajapati,Abhay Dharamsi(2022).Beneficial effect of polyherbal formulation in letrozole induced Polycystic ovarian syndrome (PCOS).Journal of Traditional and Complementary Medicine,12(6),575-583.
2. Shrikalp Deshpande,Payal Patel,Nimisha Kakadia,Gaurang Shah(2019).Effect of Vitex negundo L. seeds in letrozole induced polycystic ovarian syndrome.Journal of Traditional and Complementary Medicine,9(4),336-345.