Ginkgo biloba flavonoid glycosides in antimicrobial perspective with reference to extraction method

10.1016/j.jtcme.2017.10.003

Priyanka Sati；Praveen Dhyani；Indra Dutt Bhatt；Anita Pandey

Ginkgo biloba ； Flavonoid glycosides ； Antimicrobials ； Antioxidants ； HPLC ； Indian Himalaya

Journal of Traditional and Complementary Medicine

9卷1期（2019 / 01 / 01）

15 - 23

英文

The present study aims to investigate the effect of extraction method on the recovery of flavonoid glycosides, antimicrobials and antioxidants from Ginkgo leaves collected from six different locations in Uttarakhand, Indian Himalaya. Four extraction methods, namely maceration, reflux, shaker and soxhlet were considered, where reflux extracts showed higher antimicrobial antioxidant activity and higher content of flavonoid glycosides. The reference standards of Ginkgo flavonoid glycosides (quercetin, kaempferol and isorhamnetin) and crude extracts were tested for their antimicrobial activity against gram positive and gram negative bacteria and fungi following disc diffusion method and minimum inhibitory concentration (MIC). All the test microorganisms were observed to be inhibited significantly by Ginkgo flavonoids in plate based assays. Correlation coefficients exhibited the extent of contribution of flavonoid glycosides in antimicrobial activity and confirmed the reflux method as a potential method for extraction. Moreover, antioxidant activity as measured by DPPH assay was also found to be higher in reflux method. Significant variation (p ＜ 0.05) in the flavonoid glycosides among the locations was also observed and sample collected from GB6 location was found to be the best for quercetin and isorhamnetin, while GB5 for kaempferol. Significant correlation (r ＜ 0.05, r ＜ 0.001) was obtained while developing the relationship between total flavonoid glycosides and antimicrobials. The present study, thus suggests the reflux method of extraction to be the best for maximum recovery of flavonoid glycosides with higher antioxidant and antimicrobial activities from Ginkgo extract.

1. Bedir, ET,Khan, RA,Zhao, J(2002).Biologically active secondary metabolites from Ginkgo biloba.J Agri Food Chem,50,3150-3155.
2. Boonkaew, T,Camper, ND(2005).Biological activities of Ginkgo extracts.Phytomedicine,12,318-323.
3. Choi, JG,Jeong, SI,Ku, CS(2009).Antibacterial activity of hydroxyalkenyl salicylic acids from sarcotesta of Ginkgo biloba against vancomycin-resistant Entero-coccus.Fitoterapia,80,18-20.
4. Clinical and laboratory Standards Institute=CLSI(2005).Methods for Dilution Antimi-crobial Susceptibility Tests for Bacteria that Grow Aerobically. Approved Standard-. Sixth Edition.Wayne:
5. Clinical and laboratory Standards Institute=CLSI(2008).Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard-. Third Edition.Wayne:
6. Cushnie, TPT,Lamb, AJ(2005).Antimicrobial activity of flavonoids.Int J Antimicrob Agents,26,343-356.
7. Ebana, RUB,Madunagu, BE,Ekpe, ED,Otung, IN(1991).Microbiological exploitation of cardiac glycoside and alkaloids from Garcinia kola, Borreria ocymoides, Kola nitida and Citrus aurantifolia.J Appl Biotechnol,71,398-401.
8. Fazal, H,Ahmad, N,Ullah, I(2011).Antibacterial potential in Parthenium hysterophorus, Stevia rebaudiana and Ginkgo biloba.Pak J Bot,43,1307-1313.
9. Goh, LML,Barlow, PJ(2004).Flavonoid recovery and stability from Ginkgo biloba subjected to a simulated digestion process.Food Chem,86,195-202.
10. Hasler, A,Sticher, O.(1992).Identification and determination of the flavonoids from Ginkgo biloba by high performance liquid chromatography.J Chromatogr A,605,41-48.
11. Hayouni, EA,Abedrabba, M,Bouix, M,Hamdi, M(2007).The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts.Food Chem,105,1126-1134.
12. Kaur, P,Chaudhary, A,Singh, B(2012).Gopichand. An efficient microwave assisted extraction of phenolic compounds and antioxidant potential Ginkgo biloba.Natl Product Commun,7,203-206.
13. Kaur, P,Chaudhary, A,Singh, RD,Prasad, GRP,Singh, B(2012).Spatial and temporal variation of secondary metabolite profiles in Ginkgo biloba leaves.Chem Biodivers,9,409-417.
14. Kobus, J,Flaczyk, E,Siger, A,Nogala-Kałucka, M,Korczak, J,Pegg, RB(2009).Phenolic compounds and antioxidant activity of extracts of Ginkgo leaves.Eur J Lipid Sci Technol,111,1150-1160.
15. Lee, HS,Kim, M(2002).Selective responses of three Ginkgo biloba leaf-derived constituents on human intestinal bacteria.J Agric Food Chem,50,1840-1844.
16. Maltas, E,Vural, HC,Yildiz, S(2011).Antioxidant activity and fatty acid composition of Ginkgo biloba from Turkey.J Food Biochem,35,803-818.
17. Mazzanti, G,Mascellino, MT,Battinelli, L,Coluccia, D,Manganaro, M,Saso, L(2000).Antimicrobial investigation of semipurified fractions of Ginkgo biloba leaves.J Ethnopharmacol,71,83-88.
18. Negi, PS,Jayaprakasha, GK,Rao, LJM,Sakariah, KK(1999).Antibacterial activity of turmeric oil- A byproduct from curcumin manufacture.J Agric Food Chem,47,4297-4300.
19. Perry, NB,Anderson, RE,Brennan, NJ(1999).Essential oils from Dalmation sage (Salvia officinalis L.): variations among individuals, plant parts, seasons and sites.J Agric Food Chem,47,2048-2054.
20. Rauha, JP,Remes, S,Heinonen, M(2000).Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds.Int J Food Microbiol,56,3-12.
21. Sati, P,Pandey, A,Palni, LMS(2012).Antimicrobial potential of leaf extracts of Ginkgo biloba L., growing in Uttarakhand, India.Natl Acad Sci Lett,35,201-206.
22. Sati, P,Pandey, A,Rawat, S,Rani, A(2013).Phytochemicals and antioxidants in leaf extracts of Ginkgo biloba with reference to location, seasonal variation and solvent system.J Pharm Res,7,804-809.
23. Singh, B,Kaur, P,Gopichand Singh, RD,Ahuja, PS(2008).Biology and chemistry of Ginkgo biloba.Fitoterapia,79,401-418.
24. Tang, D,Yang, D,Tang, A(2010).Simultaneous chemical fingerprint and quantitative analysis of Ginkgo biloba extract by HPLC-DAD.Anal Bioanal Chem,396,3087-3095.
25. Tao, R,Wang, CZ,Kong, ZW(2013).Antibacterial/antifungal activity and synergistic interactions betwen polyprenols and other lipids isolated from Ginkgo biloba L. leaves.Molecules,18,216-2182.
26. Van Beek, TA(ed.)(2000).Ginkgo Biloba.Amsterdam:Harwood.
27. van Beek, TA,Montoro, P(2009).Chemical analysis and quality control of Ginkgo biloba leaves, extracts and phytopharmaceuticals.J Chromatogr A.,1216,2002-2032.
28. Wong, SP,Leong, LP,Koh, JHW(2006).Antioxidant activities of aqueous extracts of selected plants.Food Chem,99(4),775-783.
29. Xu, SL,Choi, RCY,Zhu, KY(2012).Isorhamnetin, a flavonol aglycone from Ginkgo biloba L., induces neuronal differentiation of cultured PC12 cells: potentiating the effect of nerve growth factor.J Evid Based Complement Altern Med.