中文摘要
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The present study has been carried out to evaluate antihemorrhoidal activity of bark of Acacia ferruginea DC. The total phenolic, total flavonoid and saponins were determined. Anti-hemorrhoidal potential of bark extract was determined by levels of inflammatory cytokines such as TNF-α, IL-6, PGE2 and recto-anal coefficient (RAC). The histopathological examination was done to evaluate the severity score in the treated and untreated groups. The results of phytochemical screening of the hydroalcoholic extract of A. ferruginea revealed the presence of alkaloids, flavonoids, triterpenoid, saponins, tannins and phenolic compounds The total phenolic, flavonoid and saponin contents were found to be 438.8mg/g GAE, 66.6mg/gRE and 34%w/w respectively. Hydroalcoholic extract of bark of A. ferruginea significantly reduced the inflammatory cytokines {TNF-α(8.40±0.188), IL-6(3.95±0.181), PGE2(53.27±2.956) and RAC(0.998±0.094)}as compared to positive control group{TNF-α(13.36±0.141), IL-6(7.25±0.161), PGE2(82.34±3.395) and RAC(1.131 ± 0.008)}. Noticeably the results were comparable to that of standard pilex granules {TNF-α(7.12±0.166), IL-6(3.01±0.156), PGE2(42.51±2.157) and RAC (0.968±0.084)}. Molecular docking and structure based pharmacophore mapping further confirmed the anti-inflammatory mediated antihemorrhoidal activity of the hydroalcoholic extract. The antihemorrhoidal activity of hydroalcoholic extract of A. ferruginea may attribute to the flavonoids.
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参考文献
|
-
http://www.rightdiagnosis.com/h/hemorrhoids/stats-country.htm (Accessed 23.Augest.2014).
-
Akare, SC,Sahare, AY,Shende, MA,Bondre, AV,Wanjari, AD(2009).Hepatoprotective activity of Acacia ferruginea DC. leaves against carbon tetrachloride induced liver damaged in rats.Int J Pharm Technol Res,1(3),962-965.
-
Azeemuddin, M,Viswanatha, GL,Rafiq, M(2014).An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation.ISRN Pharmacol,1-7.
-
Evans, P,Halliwell, B.(2001).Micronutrients: oxidants/antioxidants status.Br J Nutr,85(Suppl 2),S67-S74.
-
Gowri, SS,Pavitha, S,Vasantha, K(2011).Free radical scavenging capacity and antioxidant activity of young leaves and barks of Acacia nilotica (l.) Del.Int J Pharm Pharmaceut Sci,3(1),160-164.
-
Harborne, JB(1998).Phytochemical Methods.London:Chapman and Hall.
-
Iqbal, P,Ahmed, D,Asghar, MN(2014).A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica.Asian Pac J Trop Med.,7(Suppl 1),S473-S480.
-
Johanson, JF,Sonnenberg, A(1990).The prevalence of hemorrhoids and chronic constipation: an epidemiologic study.Gastroenterology,98(2),380-386.
-
LeClere, FB,Moss, AJ,Everhart, JE,Roth, HP(1992).Prevalence of major digestive disorders and bowel symptoms.Adv Data,212,1-15.
-
Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S. Agroforestree Database: A Tree Reference and Selection Guide Version 4.0. Kenya: World Agroforestry Centre; 2009. Available: http://www.worldagroforestry.org/resources/databases/agroforestree
-
Rajpal, V.(2002).Standardization of Botanicals.New Delhi:Eastern publishers.
-
Sakthivel, MK,Guruvayoorappan, C(2013).Acacia ferruginea inhibits tumor progression by regulating inflammatory mediators-(TNF-a, iNOS, COX-2, IL-1b, IL-6, IFN-g, IL-2, GM-CSF) and pro-angiogenic growth factor-VEGF.Asian Pac J Cancer Prev APJCP,14(6),3909-3919.
-
Singleton, VL,Orthofer, R,Lamuela- raventos, RM(1999).Analysis of total phenols and other oxidation substrates and antioxidants by means of folin ciocalteu reagent.Meth Enzymol,299,152-178.
-
Sowndhararajan, K,Hong, S,Jhoo, JW,Kim, S,Chin, NL(2015).Effect of acetone extract from stem bark of Acacia species (A. dealbata, A. ferruginea and A. leucophloea) on antioxidant enzymes status in hydrogen peroxide-induced HepG2 cells.Saudi J Biol Sci,22(6),685-691.
-
Sowndhararajan, K,Joseph, JM,Manian, S(2013).Antioxidant and free radical scavenging activities of Indian Acacias: Acacia leucophloea (Roxb.) Willd., Acacia ferruginea DC., Acacia dealbata Link. and Acacia pennata (L.) Willd.Int J Food Prop,16,1717-1729.
-
Sowndhararajan, K,Kang, SC(2013).Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats.J Ethnopharmacol,148(1),175-181.
-
Vahitha, R,Venkatachalam, MR,Murugan, K,Jebanesan, A(2002).Larvicidal efficacy of Pavonia zeylanica l. and Acacia ferruginea DC against Culex quinquefasciatus Say.Bioresour Technol,82(2),203-204.
-
Woisky, R,Salatino, A(1998).Analysis of propolis: some parameters and procedures for chemical quality control.J Apicult Res,37,99-105.
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