Water and ethanol extracts of Plantago major leaves show anti-inflammatory activity on oral epithelial cells

10.1016/j.jtcme.2017.09.002

Muhammad Zubair；Cecilia Widén；Stefan Renvert；Kimmo Rumpunen

Common plantain ； Herbal medicine ； NF-kB ； Wound healing

Journal of Traditional and Complementary Medicine

9卷3期（2019 / 07 / 01）

169 - 171

英文

Context: The leaves of Plantago major have been used for the treatment of wounds and inflammation in folk medicine from prehistoric times. However there is no report on the use of P. major to treat inflammation in oral epithelial cell lines. Objective: The present study was undertaken to reveal possible anti-inflammatory effects of Plantago major leaf extracts on oral epithelial cells in-vitro. Materials and methods: Water- and ethanol-based extracts of P. major leaves were prepared from freeze-dried plant material, and tested in-vitro using the oral epithelial cell line H400. The anti-inflammatory activity of P. major was tested against E. coli lipopolysaccharide (LPS) using the nuclear factor kappa beta (NF-kB) assay. Results: Both the water- and the ethanol-based extracts, as well as a combination of the two extracts, showed anti-inflammatory activity. A concentration of 0.1 mg/mL (on dry weight basis) yielded the best results for all extracts. Discussion and conclusion: The results show that synergistic effects of both polyphenols and water-soluble compounds (possibly polysaccharides) are responsible for anti-inflammatory activities of P. major.

1. Beara, IN,Orcic, DZ,Lesjak, MM,Mimica-Dukic, NM,Pekovic, BA,Popovic, MR(2010).Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of plantain (Plantago L.) species.J Pharm Biomed anal,52,701-706.
2. Mahmood, AA,Phipps, ME(2006).Wound healing activities of Plantago major leaf extract in rats.Int J Trop Med,1,33-35.
3. Miagkov, AV,Kovalenko, DV,Brown, CE(1998).NF-kappaB activation provides the potential link between inflammation and hyperplasia in the arthritic joint.Proc Natl Acad Sci U. S. A,10(95),13859-13864.
4. Milward, MR,Chapple, IL,Wright, HJ,Millard, JL,Matthews, JB,Cooper, PR(2007).Differential activation of NF-kappaB and gene expression in oral epithelial cells by periodontal pathogens.Clin Exp Immunol,148,307-324.
5. Murai, M,Tamayama, Y,Nishibe, S(1995).Phenylethanoids in the herb of Plantago lanceolata and inhibitory effect on arachidonic acid-induced mouse ear edema.Planta med,61,479-480.
6. Poynter, ME,Irvin, CG,Janssen-Heininger, YM(2003).A prominent role for airway epithelial NF-kappa B activation in lipopolysaccharide-induced airway inflammation.J Immunol,15(170),6257-6265.
7. Ren, L,Leung, WK,Darveau, RP,Jin, L(2005).The expression profile of lipopolysaccharide-binding protein, membrane-bound CD14, and toll-like receptors 2 and 4 in chronic periodontitis.J periodontol,76,1950-1959.
8. Samuelsen, AB(2000).The traditional uses, chemical constituents and biological activities of Plantago major L. A review.J Ethnopharmacol,71,1-21.
9. Sharif, O,Bolshakov, VN,Raines, S,Newham, P,Perkins, ND(2007).Transcriptional profiling of the LPS induced NF-kappaB response in macrophages.BMC Immunol,8,1.
10. Zubair, M,Ekholm, A,Nybom, H,Renvert, S,Widen, C,Rumpunen, K(2012).Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay.J Ethnopharmacol,14(141),825-830.
11. Zubair, M,Nybom, H,Ahnlund, M,Rumpunen, K(2012).Detection of genetic and phytochemical differences between and within populations of Plantago major L. (plantain).Sci Hortic,136,9-16.
12. Zubair, M,Nybom, H,Lindholm, C,Rumpunen, K(2011).Major polyphenols in aerial organs of greater plantain (Plantago major L.), and effects of drying temperature on polyphenol contents in the leaves.Sci Hortic,128,523-529.

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