Evaluation of α-amylase, lipase inhibition and in-vivo pharmacological activities of Eucalyptus camaladulensis Dehnh leaf extract

10.1016/j.jtcme.2018.07.001

Anil Upreti；Bibek Byanju；Muna Fuyal；Apekshya Chhetri；Paras Pandey；Rajeswar Ranjitkar；Jyoti Joshi Bhatta；Bishnu Prasad Pandey

Eucalyptus camaladulensis Dehnh ； Pharmacological activity ； Lipase activity ； Amylase ； Anti-ulcer ； Anti-diarrhea ； Anti-inflammatory ； Analgesic activity

Journal of Traditional and Complementary Medicine

9卷4期（2019 / 10 / 01）

312 - 318

英文

In this present study, phytochemical screening, anti-ulcer assay, anti-diarrhea assay, anti-inflammatory assay, analgesic assay, lipase activity assay, amylase activity assay and the anti-bacterial activity of Eucalyptus camaladulensis Dehnh leaf extracted with methanol and 50% ethanol was analyzed for biological significance. Physical characterization of the non-volatile component revealed the higher yield of 16.92% in 50% ethanol expediting the use of 50% ethanol as a better alternative. Further use of crude extract revealed 33.89% (IC_(50)=1.44mg/ml) of α-amylase inhibition by methanol extract and 33.87% (IC_(50)=3.21mg/ml) lipase inhibition by 50% ethanol extract. Furthermore, 44.44% protective ratio towards ulcer was observed with the methanol extract, whereas 54.58% anti-inflammatory activity was shown by the 50% ethanol extract. The effectiveness of the extract was further enhanced by the presence of 62.54% motility and best analgesic property at 180 min of the exposure of the extract orally. The antioxidant activity of crude methanol extract revealed an IC_(50) value 601.8 μg/ml whereas, ethanol extract showed 1279.58 μg/ml in DPPH assay. Result revealed several health benefits of E. camaldulensis Dehnh leaf.

1. Ahmed, M(2015).Acute toxicity (lethal dose 50 calculation) of herbal drug somina in rats and mice.Pharmacol Pharm,6(6),185-189.
2. Alcaraz, MJ,Hoult, JR(1985).Actions of flavonoids and the novel anti-inflammatory flavone, hypolaetin-8-glucoside, on prostaglandin biosynthesis and inactivation.Biochem Pharmacol,34(14),2477-2482.
3. Alibabaei, zahra,Pilehvarian, A,Shirani, M(2010).Effect of Euphorbia helioscopia on acetic acid-induced abdominal constrictions in Balb/c mice.J Shahrekord Univ Med Sci,11(4),9-14.
4. Arun, M,Asha, VV(2008).Gastroprotective effect of Dodonaea viscosa on various experimental ulcer models.J Ethnopharmacol,118(3),460-465.
5. Beisson, F,Tiss, A,Rivière, C,Verger, R(2000).Methods for lipase detection and assay: a critical review.Eur J Lipid Sci Technol,133-153.
6. Birben, E,Sahiner, UM,Sackesen, C,Erzurum, S,Kalayci, O(2012).Oxidative stress and antioxidant defense.World Allergy Organ J,5(1),9-19.
7. Chitme, HR,Chandra, M,Kaushik, S(2004).Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals.J Pharm Pharmaceut Sci.,7(1),70-75.
8. Davenport, HW(1969).Gastric mucosal hemorrhage in dogs: effects of acid, aspirin, and alcohol.Gastroenterology,56(563),439-449.
9. Dhakad, AK,Pandey, VV,Beg, S,Rawat, JM,Singh, A(2018).Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review.J Sci Food Agric.,98(3),833-848.
10. Grattapaglia, D,Vaillancourt, RE,Shepherd, M(2012).Progress in Myrtaceae genetics and genomics: Eucalyptus as the pivotal genus.Tree Genet Genomes,8,463-508.
11. Hockett, KL,Baltrus, DA(2017).Use of the soft-agar overlay technique to screen for bacterially produced inhibitory compounds.JoVE,119
12. Hussain, MS,Rahman, MA,Fareed, S,Ansari, S,Ahmad, I.,Saeed, Mohd(2012).Current approaches toward production of secondary plant metabolites.J Pharm Bio-Allied Sci.,4(1),10.
13. Imam, H,Mahbub, NU,Khan, MF,Hana, HK,Sarker, MMR(2013).Alpha amylase enzyme inhibitory and anti-inflammatory effect of Lawsonia inermis.Pakistan J Biol Sci PJBS,16(23),1796-1800.
14. Khennouf, Seddik,Benabdallah, Hassiba,Gharzouli, Kamel(2003).Effect of Tannins from Quercus suber and Quercus Coccifera Leaves on Ethanol-induced Gastric Lesions in Mice.J. Agric. Food Chem.,51(5)
15. Kim, J-S(2005).Radical scavenging capacity and antioxidant activity of the E vitamer fraction in rice bran.J Food Sci.,70(3),C208-C213.
16. Lewis, DA(1989).Anti-inflammatory drugs from plant and marine sources.Agents Actions Suppl,27,3-373.
17. Lunagariya, NA,Patel, NK,Jagtap, SC,Bhutani, KK(2014).Inhibitors of pancreatic lipase: state of the art and clinical perspectives.EXCLI J.,13,897-921.
18. Mohamed, EAH,Siddiqui, MJA,Ang, LF(2012).Potent a-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism.BMC Compl Alternative Med,12(1),1193.
19. Mulyaningsih, S,Sporer, F,Zimmermann, S,Reichling, J,Wink, M(2010).Synergistic properties of the terpenoids aromadendrene and 1,8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens.Phytomedicine,17(13),1061-1066.
20. Paguigan, ND,Castillo, DHB,Chichioco-Hernandez, CL.Anti-ulcer activity of leguminosae plants.Arq Gastroenterol,51(1),64-67.
21. Paul, S,Saha, D(2012).Anti-inflammatory and analgesic activity of stem bark of moringa oleifera.' pharmacologyonline.Asian J Res Pharm Sci,2(2),95-97.
22. Pham-Huy, LA,He, H,Pham-Huy, C(2008).Free radicals, antioxidants in disease and health.Int J Biomed Sci.,4(2),89-96.
23. Pladio, LP,Villasñnor, I.Anti-spasmodic constituents from drimys piperita hook F. Leaves.Philipp J Sci
24. Qabaha, K,Ras, SA,Abbadi, J,Al-Rimawi, F(2016).Anti-inflammatory activity of eucalyptus spp. and pistascia lentiscus leaf extracts.African J Tradit Complement Altern Med,13(5),1-6.
25. Rahimzadeh, M,Jahanshahi, S,Moein, S,Moein, MR(2014).Evaluation of alpha- amylase inhibition by Urtica dioica and Juglans regia extracts.Mashhad Univ Med Sci.,17(6),465-469.
26. Rahman, MK,Barua, S,Islam, MF(2013).Studies on the anti-diarrheal properties of leaf extract of Desmodium puchellum.Asian Pac J Trop Biomed,3(8),639-643.
27. Ramezani, M,Amin, G,Jalili, E(2010).Antinociceptive and anti-inflammatory effects of hydroalcoholic extract of Vitex agnus castus fruit in mice.J Shahrekord Univ Med Sci.,11(4),46-51.
28. Sebei, K,Sakouhi, F,Herchi, W,Khouja, ML,Boukhchina, S.(2015).Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves.Biol Res,48(1),7.
29. Serafini, M,Peluso, I,Raguzzini, A(2010).Flavonoids as anti-inflammatory agents.Proc Nutr Soc.,69(3),273-278.
30. Sharma, P,Vidyasagar, G,Singh, S,Ghule, S,Kumar, B(2010).Antidiarrhoeal activity of leaf extract of celosia argentea in experimentally induced diarrhoea in rats.J Adv Pharm Technol Res,1(1),41-48.
31. Shoaib, M,Shah, I,Ali, N,Shah, WA(2016).A mechanistic approach to anti-nociceptive potential of Artemisia macrocephala Jacquem.BMC Compl Alternative Med,16(1),141.
32. Silva, J,Abebe, W,Sousa, SM,Duarte, VG,Machado, MIL,Matos, FJA(2003).Analgesic and anti-inflammatory effects of essential oils of Eucalyptus.J Ethnopharmacol,89(2-3),277-283.
33. Singab, AN,Ayoub, N,Alsayed, E,Martiskainen, O,Sinkkonen, J,Pihlaja, K(2011).Phenolic constituents of Eucalyptus camaldulensis Dehnh, with potential antioxidant and cytotoxic activities.Record Nat Prod,5,271-280.
34. Viana, AFSC,Fernandes, HB,Silva, FV(2013).Gastroprotective activity of Cenostigma macrophyllum Tul. var. acuminata Teles Freire leaves on experimental ulcer models.J Ethnopharmacol,150(1),316-323.
35. Yadav, R,Agarwala, M(2011).Phytochemical analysis of some medicinal plants.J Phytol,3(12),10-14.

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