Chromatographic fractions from Chrysophyllum albidum stem bark boost antioxidant enzyme activity and ameliorate some markers of diabetes complications

10.1016/j.jtcme.2021.01.005

Babalola Ola Yusuf；Musa Toyin Yakubu；Musbau Adewumi Akanji

Alloxan ； Dyslipidemia ； Glycosylated haemoglobin ； C-reactive protein ； Chrysophyllum albidum

Journal of Traditional and Complementary Medicine

11卷4期（2021 / 07 / 01）

336 - 342

英文

Background and aim: Diabetes, with hyperglycaemia as hallmark, is a global crisis that reduces the antioxidant status and produces complications when poorly managed. The development of complications can be indicated by inflammation, lipid peroxidation and the accumulation of glycation adducts. Thus, the attenuation of hyperglycaemia and boosting of antioxidants status is key in ameliorating markers of diabetes complications. This work evaluated the potency of Chrysophyllum albidum stem bark on some markers of diabetes complications. Experimental procedure: A total of 100 female rats (180.80 ± 8.50 g) were assigned into ten groups of ten animals each; control received 1.0 ml of distilled water while those in groups DC, RD, F1, F3, F4, F5, F7, F9, F10 were induced into diabetes by intraperitoneal administration of 120 mg/kg body weight of alloxan and were orally administered distilled water, glibenclamide, 2.5 mg/kg of the chromatographic fractions 1, 3, 4, 5, 7, 9, and 10 respectively, once daily for 14 days. F7 was profiled for its bioactive constituents and the pancreas histology of the rats were examined. Results: Chromatographic fractions F5 and F7 significantly decreased fasting blood glucose, glycosylated haemoglobin, C-reactive protein, total cholesterol, triglycerides, atherogenic index, malondialdehyde while insulin, high density lipoprotein, catalase, superoxide dismutase activities significantly increased. Fraction F7 revealed eight compounds and restored the distorted pancreas. Conclusion: Fraction F7 ameliorated the markers of diabetes complications considered in this study better than F5, restored the compromised pancreas and can be explored as lead candidate for production of drug for the management of diabetes.

1. (1999).Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.Elsevier Saunders.
2. (1980).Carleton’s Histological Techniques.Oxford University Press.
3. Ã, AK(2009).Anzer honey prevents N -ethylmaleimide-induced liver damage in rats.Experimental and Toxicologic Pathology,61,333-337.
4. Adebayo, AH,Abolaji, AO,Opata, TK,Adegbenro, IK(2010).Effects of ethanolic leaf extract of Chrysophyllum albidum G . on biochemical and haematological parameters of albino Wistar rats.African Journal of Biotechnology,9(14),2145-2150.
5. Beers, RF,Sizer, IW(1952).A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.J Biol Chem,195(1),133-140.
6. Bhutto, MG,Lokesh, MR,Sharath, KDS,Afroze, MKH,Ghouse, PAD(2017).Association between lipid profile and silent coronary artery disease in south Indian patients with type 2 diabetes mellitus.Int J Adv Med,4(1)
7. Danne, T,Weber, B,Hartmann, R,Enders, I,Burger, W,Hovener, G(1994).Long-term glycemic control has a nonlinear association to the frequency of background retinopathy in adolescents with diabetes: follow-up of the Berlin retinopathy study.Diabetes Care,17(12),1390-1396.
8. Friedewald, WT,Levy, RI,Fredrickson, DS(1972).Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge.Clin Chem,18(6),499-502.
9. Hunt, JV,Dean, RT,Wolff, SP(1988).Hydroxyl radical production and autoxidative glycosylation. Glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing.Biochem J,256(1),205-212.
10. Idowu, TO,Ogundaini, AO,Adesanya, SA(2016).Isolation and characterization of chemical constituents from Chrysophyllum albidum G. Don-Holl. stem-bark extracts and their antioxidant and antibacterial properties.Afr J Tradit Complement Altern Med,13(5),182-189.
11. Jacobson, TA,Miller, M,Schaefer, E(2007).Hypertriglyceridemia and Cardiovascular Risk Reduction.Clin Ther,29(5),763-777.
12. Kehlenbrink, S,Koppaka, S,Martin, M,Relwani, R(2012).Elevated NEFA levels impair glucose effectiveness by increasing net hepatic glycogenolysis.Diabetologia,2012,3021-3028.
13. King, GL.(2008).The role of inflammatory cytokines in diabetes and its complications.J Periodontol,79(8s),1527-1534.
14. Lee, J,Lee, HI,Seo, K, Il(2014).Effects of ursolic acid on glucose metabolism, the polyol pathway and dyslipidemia in non-obese type 2 diabetic mice.Indian J Exp Biol,52(7),683-691.
15. Lopes-Virella, MF,Stone, P,Ellis, S.,C, JA(1977).Cholesterol determination in high-density lipoproteins separated by three different methods.Clin Chem,23(5),882-884.
16. Mahluji, S,Ostadrahimi, A,Mobasseri, M,Attari, VE,Payahoo, L(2013).Anti-inflammatory effects of zingiber officinale in type 2 diabetic patients.Adv Pharm Bull,3(2),273-276.
17. Mirochnik, R,Araida, S,Yaffe, V,El-naaj, IA(2017).C-reactive protein concentration as a prognostic factor for in fl ammation in the management of odontogenic infections.Br J Oral Maxillofac Surg,1-5.
18. Misra, HP,Fridovich, I(1972).The role of superoxide dismutase anion in the auto oxidation of epinephrine and a simple assay for superoxide dismutase.J Biochem,247,3170-3175.
19. Mohamed, M,Sulaiman, SA,Jaafar, H,Sirajudeen, KNS(2012).Effect of different doses of Malaysian honey on reproductive parameters in adult male rats.Andrologia,182-186.
20. Muhit, MA,Tareq, SM,Apu, AS(2010).BD and IM. Isolation and identification of compounds from the leaf extract of Dillenia indica Linn.Bangladesh Pharm J.,13(1),49-53.
21. Newsholme, P,Cruzat, V,Arfuso, F,Keane, K(2014).Nutrient Regulation of Insulin Secretion and Action.Journal of Endocrinology,221
22. Okoli, BJ,Okere, OS(2010).Antimicrobial activity of the phytochemical constituents of Chrysophyllum albidum G.Don_Holl. (African star apple) plant.J Res Natl Dev,8(1),301-311.
23. Oloyede, HOB,Bello, TO,Ajiboye, TO,Salawu, MO(2015).Antidiabetic and anti-dyslipidemic activities of aqueous leaf extract of Dioscoreophyllum cumminsii (Stapf) Diels in alloxan-induced diabetic rats.J Ethnopharmacol,166,313-322.
24. Onyeka, CA,Aligwekwe, AU,Olawuyi, TS,Nwakanma, AA,Kalu, ECOA(2012).Antifertility effects of ethanolic root bark extract of Chrysophyllum albidum in male albino rats.Int J Appl Res Nat Prod,5(1),12-17.
25. Pepys, MB,Hirschfield, GM(2003).C-reactive protein: a critical update.J Clin Invest,111(12),1805-1812.
26. Petersen, MC,Vatner, DF,Shulman, GI(2017).Regulation of hepatic glucose metabolism in health and disease.Nat Rev Endocrinol,13(10),572-587.
27. Pickering, RJ,Rosado, CJ,Sharma, A,Buksh, S,Tate, M,de Haan, JB(2018).Recent novel approaches to limit oxidative stress and inflammation in diabetic complications.Clin Transl Immunol,7(4),1-20.
28. Roeschlau, P,Bernt, E,Gruber, JW(1974).Biochem Clin Chem Clin,2,403.
29. Sasikala, C,Sudhakar, Y(2015).Effect of compounds isolated from filicium decipiens and ventilago madraspatana against diabetic nephropathy in streptozotocin induced diabetic rats.Indian J Pharm Educ Res,49(2),146-151.
30. Schmidt, AM,Editor, G,Giacco, F,Brownlee, M(2010).Oxidative stress and diabetic complications IR RAGE SOD.Circulation Research,107(9),842-853.
31. Schultz, CJ,Konopelska-Bahu, T,Dalton, RN(1999).Microalbuminuria prevalence varies with age, sex, and puberty in children with type 1 diabetes followed from diagnosis in a longitudinal study.Diabetes Care,22(3),495-502.
32. Sharma, AK,Bharti, S,Goyal, S(2011).Upregulation of PPARg by Aegle marmelos ameliorates insulin resistance and β-cell dysfunction in high fat diet fed-streptozotocin induced type 2 diabetic rats.Phyther Res,25(10),1457-1465.
33. Spranger, J,Kroke, A,Mo, M,Hoffmann, K,Bergmann, MM(2003).Inflammatory cytokines and the risk to develop type 2 diabetes.Diabetes,52,812-817.
34. Tiwari, BK,Pandey, KB,Abidi, AB,Rizvi, SI(2013).Markers of Oxidative Stress during Diabetes Mellitus.Journal of biomarkers,2013
35. Tomlinson, DR,Gardiner, NJ(2008).Glucose neurotoxicity.Nature Reviews Neuroscience,9
36. Trinder, P(1969).Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor.Ann Clin Biochem,6(1),24-27.
37. Trivelli, LA,Ranney, HM,Lai, H-T(1971).Hemoglobin components in patients with diabetes mellitustle.N Engl J Med,284(7),353-357.
38. Ullah, A.(2016).Diabetes mellitus and oxidative stress -- A concise review.Saudi Pharmaceut J,24(5),547-553.
39. Varshney, R,Kale, RK(1990).Effects of Calmodulin antagonists on radiation-induced lipid peroxidation in microsomes.Int J Radiat Biol,58,733-743.
40. Weber, B,Burger, W,Hartmann, R,Hovener, GMR,O, U(1986).Risk factors for the development of retinopathy in children and adolescents with Type 1 (insulin-dependent) diabetes mellitus.Diabetologia,29,23-29.
41. Wolff, SP,Dean, RT(1987).Glucose autoxidation and protein modification. The potential role of "autoxidative glycosylation" in diabetes.Biochem J,245(1),243-250.
42. Yakubu, MT,Akanji, MA,Oladiji, AT(2005).Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats.Asian J Androl,7(4),399-404.
43. Yakubu, MT,Ogunro, OB(2014).Effects of aqueous extract of Fadogia agrestis stem in alloxan-induced diabetic rats.Bangladesh J Pharmacol,9(3),356-363.
44. Yaribeygi, H,Atkin, SL,Sahebkar, A(2019).A review of the molecular mechanisms of hyperglycemia-induced free radical generation leading to oxidative stress.J Cell Physiol,234(2),1300-1312.
45. Yusuf, BO,Yakubu, MT,Akanji, MA(2020).Anti-diabetic activity of Chrysophyllum albidum (G. Don) stem bark in alloxan-induced type 1 diabetic female wistar rats.Tanzan J Sci.,6(3)