题名

Preventing c2c12 muscular cells damage combining magnesium and potassium with vitamin D3 and curcumin

DOI

10.1016/j.jtcme.2021.05.003

作者

Claudio Molinari；Sara Ruga；Mahitab Farghali；Rebecca Galla；Ahmad Bassiouny；Francesca Uberti

关键词

C2C12 cells ； Fatigue ； Muscle recovery ； Nutraceuticals ； Physical activity

期刊名称

Journal of Traditional and Complementary Medicine

卷期/出版年月

11卷6期（2021 / 11 / 01）

页次

532 - 544

内容语文

英文
中文摘要

Background and aim: Physical activity is defined as any bodily movement produced by skeletal muscles which causes energy consumption; moderate and constant physical activity is known to be beneficial and to slow the muscle loss process associated with aging. The aim of the present study was to test, in an in vitro exercise model, the biological effects of a new formulation composed of magnesium and potassium combined with vitamin D and curcumin created to support muscle activity and to prevent hypercontraction damage. Experimental procedure: C2C12 cells were treated with vitamin D, buffered magnesium bisglycinate, curcumin, and potassium citrate. Cell viability, morpho-functional changes, calcium and magnesium movements, and the main kinases involved in glucose uptake were analyzed. The glycogen level and lactate were also evaluated. Results and conclusion: Important results about a positive effect on mitochondrial activity, ATP production, oxygen consumption and in the physiological differentiation of C2C12 cells were obtained. Further experiments were performed under conditions that mimic the biological aspects of strenuous exercise. The combination of magnesium, vitamin D3, curcumin, and potassium citrate revealed beneficial effects on skeletal muscle cells under physiological conditions as well as while mimicking intense activity. In particular, in an in vitro model, they were able to control the hypercontraction, restoring ion fluxes, reducing inflammation signaling and supporting the main mechanism involved on aerobic activity. Our results have indicated for the first time that this new combination could be considered as a new nutraceutical formulation to improve physical performance and muscle recovery.
主题分类 醫藥衛生 > 中醫藥學
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