题名

The effect of combining basil seeds and gum Arabic on the healing process of experimental acetic acid-induced ulcerative colitis in rats

DOI

10.1016/j.jtcme.2022.08.001

作者

Mohammad Abbas Bejeshk；Amir Hashem Aminizadeh；Mohammad Amin Rajizadeh；Mohammad Khaksari；Mahdiyeh Lashkarizadeh；Nader Shahrokhi；Mohammad Javad Zahedi；Maryam Azimi

关键词

Basil seeds ； Gum Arabic ； Acetic acid-induced ulcerative colitis ； Ulcerative colitis ； Ocimum basilicum ； Acacia Senegal

期刊名称

Journal of Traditional and Complementary Medicine

卷期/出版年月

12卷6期（2022 / 11 / 01）

页次

599 - 607

内容语文

英文
中文摘要

Background & aim: Ulcerative colitis (UC) is a chronic recurrent inflammatory disease of the large intestine and rectum that oxidative stress and severe inflammation are the main features of this disease. Previous studies have shown that separate consumption of basil and gum arabic can reduce inflammation and oxidative stress. The aim of the study was evaluating the effect of treatment with basil seeds given together with gum arabic on healing, inflammation and oxidative stress in the course of experimental colitis in rats. Experimental procedure: A total number of 50 male rats were used, randomly assigned to five groups of 10 rats each. Colitis was induced in rats by enemas with 4% solution od acetic acid. Four days after induction of colitis, rats were treated for next 4 days with saline or combination of basil seeds plus gum arabic (1 mg/kg) or sulfasalazine (100 mg/g) rectally. The experiment was terminated after last dose of treatment. Rats without induction of colitis were used as a sham group. Results: Acetic acid-induced colitis increased the macroscopic and histopathological damage scores of the colon as well as colon levels of MDA(Malondialdehyde), MPO(Myeloperoxidase), TNFα(Tissue necrosis factor α), IL6 (Interleukin 6)and IL17(Interleukin 17) and decreased SOD(Superoxide Dismutase), GPx (Glutathione Peroxidase) and IL10 (Interleukin 10) levels compared with the control group(P ＜ 0.001). Treatment with basil and gum arabic reduced macroscopic and histopathological damage scores (P ＜ 0.01) of the colon, MDA, MPO, TNFα, IL6(P ＜ 0.001) and IL17 (P ＜ 0.01) levels of the colon and increased SOD, GPx and IL10 levels compared to the colitis group (P ＜ 0.01). Conclusion: Rectal administration of combination of basil seeds plus gum arabic after induction of colitis, exhibits antioxidant and anti-inflammatory effects, and accelerates the healing of the colon in experimental colitis evoked by acetic acid.
主题分类 醫藥衛生 > 中醫藥學
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