Electroacupuncture at GB34 modulates neurogenesis and BDNF-ERK signaling in a mouse model of Parkinson's disease

10.1016/j.jtcme.2023.01.005

Yukyoung Lee；Hanul Lee；Chang-Hwan Bae；Ji Eun Seo；Hee-Young Kim；Sungtae Koo；Seungtae Kim

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine ； Acupuncture ； Extracellular signal-regulated kinase ； Neurogenesis ； Parkinson's disease

Journal of Traditional and Complementary Medicine

13卷3期（2023 / 05 / 01）

263 - 269

英文

Background and aim: It has been reported that acupuncture at GB34 can enhance neurogenesis in the subventricular zone (SVZ) of mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). However, the signaling pathway that plays a critical role in neurogenesis needs to be established. Herein, we investigated the neurogenesis-promoting pathway mediated by acupuncture, focusing on extracellular signal-regulated kinase (ERK) signaling. Experimental procedure: Male 10-week-old C57BL/6 mice were intraperitoneally injected with 30 mg/kg MPTP once daily for 5 days. Subsequently, mice were intraperitoneally injected with 50 mg/kg bromo-deoxyuridine (BrdU), and electroacupuncture (EA) was performed at GB34 and BL60 for 3 weeks. The survival of dopaminergic neurons in the nigrostriatal pathway, cell proliferation in the SVZ, and expression levels of brain-derived neurotrophic factor (BDNF) and phosphorylated ERK (pERK) were evaluated. Results and conclusion: MPTP induced dopaminergic neuronal death in the nigrostriatal pathway, and reduced the number of BrdU-positive and BrdU/doublecortin double-positive cells in the SVZ; these parameters were restored by EA. Moreover, EA prevented MPTP-induced reduction in striatal expression of BDNF and pERK. These results indicate that EA could prevent dopaminergic neuronal death in the nigrostriatal pathway and restore neurogenesis in the SVZ, which may be attributed to the activation of the BDNF-ERK pathway.

1. Brown, JP,Couillard-Despres, S,Cooper-Kuhn, CM,Winkler, J,Aigner, L,Kuhn, HG(2003).Transient expression of doublecortin during adult neurogenesis.J Comp Neurol,467(1),1-10.
2. Fahn, S.(2003).Description of Parkinson's disease as a clinical syndrome.Ann N Y Acad Sci,991,1-14.
3. Hwang, TY,Song, MA,Ahn, S(2019).Effects of combined treatment with acupuncture and Chunggan formula in a mouse model of Parkinson's disease.Evid Based Complement Alternat Med,2019,3612587.
4. Jang, JH,Yeom, MJ,Ahn, S(2020).Acupuncture inhibits neuroinflammation and gut microbial dysbiosis in a mouse model of Parkinson's disease.Brain Behav Immun,89,641-655.
5. Jeon, H,Ryu, S,Kim, D,Koo, S,Ha, KT,Kim, S(2017).Acupuncture stimulation at GB34 restores MPTP-induced neurogenesis impairment in the subventricular zone of mice.Evid Based Complement Alternat Med,2017,3971675.
6. Jeon, S,Kim, YJ,Kim, ST(2008).Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a Parkinson's disease mouse model.Proteomics,8(22),4822-4832.
7. Kalia, LV,Lang, AE(2015).Parkinson's disease.Lancet,386(9996),896-912.
8. Kwon, S,Seo, BK,Kim, S(2016).Acupuncture points for treating Parkinson's disease based on animal studies.Chin J Integr Med,22(10),723-727.
9. Lee, Y,Choi, G,Jeon, H(2018).Acupuncture stimulation at GB34 suppresses 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced oxidative stress in the striatum of mice.J Physiol Sci,68(4),455-462.
10. Li, S,Gu, P,Tu, W(2017).Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain.Zhongguo Zhen Jiu,37(4),411-416.
11. Lin, JG,Chen, CJ,Yang, HB,Chen, YH,Hung, SY(2017).Electroacupuncture promotes recovery of motor function and reduces dopaminergic neuron degeneration in rodent models of Parkinson's disease.Int J Mol Sci.,18(9),1846.
12. Marxreiter, F,Regensburger, M,Winkler, J(2013).Adult neurogenesis in Parkinson's disease.Cell Mol Life Sci,70(3),459-473.
13. Ou, Z,Pan, J,Tang, S(2021).Global trends in the incidence, prevalence, and years lived with disability of Parkinson's disease in 204 countries/territories from 1990.Front Public Health,9,776847.
14. Park, HJ,Lim, S,Joo, WS(2003).Acupuncture prevents 6-hydroxydopamine-induced neuronal death in the nigrostriatal dopaminergic system in the rat Parkinson's disease model.Exp Neurol,180(1),93-98.
15. Park, JY,Park, JJ,Jeon, S(2014).From peripheral to central: the role of ERK signaling pathway in acupuncture analgesia.J Pain,15(5),535-549.
16. Shohayeb, B,Diab, M,Ahmed, M,Ng, DCH(2018).Factors that influence adult neurogenesis as potential therapy.Transl Neurodegener,7,4.
17. Tejeda, GS,Diaz-Guerra, M(2017).Integral characterization of defective BDNF/TrkB signalling in neurological and psychiatric disorders leads the way to new therapies.Int J Mol Sci.,18(2),268.
18. Wang, JY,Chen, SP,Gao, YH,Qiao, LN,Zhang, JL,Liu, JL(2015).Effect of repeated lectroacupuncture intervention on hippocampal ERK and p38MAPK signaling in neuropathic pain rats.Evid Based Complement Alternat Med,2015,641286.
19. Winner, B,Winkler, J.(2015).Adult neurogenesis in neurodegenerative diseases.Cold Spring Harbor Perspect Biol,7(4),a021287.
20. Yang, L,Yue, N,Zhu, X(2013).Electroacupuncture upregulates ERK signaling pathways and promotes adult hippocampal neural progenitors proliferation in a rat model of depression.BMC Compl Alternative Med,13,288.
21. Zhao, Y,Zhang, Z,Qin, S(2021).Acupuncture for Parkinson's disease: efficacy evaluation and mechanisms in the dopaminergic neural circuit.Neural Plast,2021,9926445.
22. Zuo, Y,Wang, J,Enkhjargal, B(2019).Neurogenesis changes and the fate of progenitor cells after subarachnoid hemorrhage in rats.Exp Neurol,311,274-284.