1,8-Cineol Attenuated AB_(25-35)-Induced PC12 Cell Injury through Reducing Caspase 3 Expression and NO Production

10.6913/MRHK.201912_1(1).0002

Zhang Ze-qin；Li Hai-jian；Li Wan-zhong；Wang Lin；Li Zhen-zhen；Zhao Chun-zhen

Amyloid beta ； cineol ； caspase 3

Medical Research

1卷1期（2019 / 12 / 31）

12 - 17

英文

Objective: To investigate the effect of 1,8-cineol on caspase 3 expression and NO production induced by Aβ_(25-35) in PC12 cells. Methods: PC12 cells were cultured in vitro, and cell injury was induced by Aβ_(25-35) with a concentration of 20 μM. 1,8-cineol (1, 3, 10 μM) was pretreated before Aβ_(25-35) treatment. PC12 cell viability was evaluated by MTT detection assay. Caspase 3 protein expression was detected by Western blotting. The level of NO production in PC12 cells was measured using ELISA detection assay kit. Results: In cultured PC12 cells in vitro, MTT results showed that 20 μM of Aβ_(25-35) reduced cell viability significantly compared with control group. The cell viability was increased by pretreatment with 1,8- cineol with concentrations of 3 and 10 μM compared with Aβ_(25-35) only group. Western blotting results showed compared with control group, caspase 3 expression was increased significantly in 20 μM Aβ_(25-35) group. Compared with Aβ_(25-35) group, 1,8-cineol of 3 and 10 μM group reduced caspase 3 protein expression significantly. The level of NO production in PC12 cells was increased significantly, which was decreased by pretreatment with 3 and 10 μM of 1,8-cineol. Conclusions: Our results revealed a protective effect of 1,8-cineol on Aβ_(25-35) induced PC12 cell injury through inhibition of caspase 3 expression and NO production.