不同產地餘甘子提取物抗發炎效果之研究

Study on the Anti-inflammatory Effects of Extracts of Phyllanthus Emblica from Different Origins

10.6763/IJTCMHP.202205_6(1).0003

林文宏(Lin, Wen-Hung)；林穎志(Lin, Ying-Chih)；蔡弼合(Tsai, Bi-He)；劉家祐(Liu, Chia-Yu)；邱語溱(Chiu, Yu-Chen)；郭恆宏(Kuo, Heng-Hung)

餘甘子 ； 炎性因子 ； 巨噬細胞 ； emblica fruit (Phyllanthus emblica L.) ； inflammatory factors ； macrophage

中醫本草健康促進期刊

6卷1期（2022 / 05 / 15）

37 - 64

繁體中文

本研究選擇中國雲南、印度古吉拉特邦以及柬埔寨金邊，三個地區所生產的餘甘子（Phyllanthus emblica L.）為材料，研究其提取物對細胞抗發炎的「應答效果」。本研究以三種（常見／主要）促炎因子，包含：「一氧化氮（nitric oxide, NO）」、「白介素 6（interleukin 6, IL-6）」和「腫瘤壞死因子-α（tumor necrosis factor-alpha, TNF-α）」進行分析，並探討餘甘子提取物是否具有細胞毒性（microculture tetrazolium assay, MTT assay）。分析結果顯示，三個地區產出的餘甘子提取物，具有顯著抑制NO與IL-6產生的抗炎應答效果，但對TNF-α無顯著的抑制作用；同時，並未測得對細胞產生毒性。本研究結果，可以提供餘甘子做為抗發炎原料在產地來源選擇上決策的依據，以及商業化生產行銷的重要參考。

This study used emblica fruit (Phyllanthus emblica L.) extractions from Yunnan, China, Gujarat, India and Phnom Penh, Cambodia as materials to explore its anti-inflammatory response effect on cells. Causing inflammation of three factors, include nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), be tested and cytotoxicity (microculture tetrazolium assay, MTT assay) be analyzed. The results showed that the three of Phyllanthus emblica extraction have significantly inhibited effect on NO and IL-6. But it has no inhibitory effect on TNF-α. Meanwhile, cytotoxicity did not be detected at this study. This study can provide a gate for follower of emblica fruit to further research.

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