應用中草藥乳頭藥浴劑防治乳牛乳房炎之研究

Effect of Anti-inflammatory Herbs Dipping Agents on the Prevention and Treatment of Bovine Mastitis

10.6763/IJTCMHP.202205_6(1).0001

李國華(Lee, Kuo-Hua)；陳志毅(Chen, Jih-Yi)；趙俊炫(Chao, Chun-Hsuan)；郭恆宏(Kuo, Heng-Hung)；林文宏(Lin, Wen-Hung)

乳牛 ； 乳房炎 ； 乳頭藥浴 ； Dairy Cow ； Mastitis ； Teat dip

中醫本草健康促進期刊

6卷1期（2022 / 05 / 15）

5 - 20

繁體中文

本研究應用具抗發炎活性之中草藥萃取液調製成乳牛乳頭藥浴劑，以期達降低生乳體細胞數及防治乳牛乳房炎之目的。試驗流程是將蒲公英等中草藥進行萃取，再添加賦形劑調製成乳牛乳頭藥浴劑，進行動物臨床試驗，試驗牛隻每日早晚兩次擠乳後進行乳頭藥浴，試驗組採用中草藥乳頭藥浴劑，對照組則採用優碘乳頭藥浴劑，同場同期進行為期14天，於試驗前及試驗後採集每頭受試牛隻的乳樣，測其體細胞數，以評估對乳牛乳房炎之防治效果。結果顯示，優碘對照組（n=20）平均體細胞數，由30.8 ± 23.2 萬/mL上升為35.6 ± 31.5 萬/mL，而中草藥萃取液試驗組（n=20）平均體細胞數，則從32.2 ± 24.8 萬/mL下降為21.4 ±18.5 萬/mL。以上結果顯示，本試驗研製之抗發炎乳頭藥浴劑，對降低生乳體細胞數，深具意義與發展潛能。

The purpose of this study was application of anti-inflammatory herbs dipping agents, used in dairy teat after milking, for reducing the somatic cell count (SCC) in raw milk as well as prevent and treat the mastitis. The experimental process was to extract Chinese herbs such as dandelion, and then add excipients to prepare a teat dipping agent for clinical trials. The 40 milking cows were randomly divided into two groups, one was the control group: using betadine teat dipping agent, another was experimental group: using Chinese herbs teat dipping agent. All cows were given a teat dipping after milking twice a day in the morning and evening. The same period was carried out for 14 days. The milk samples were collected before and after the trial, and the SCC and milk ingredients were measured to evaluate the effect of prevention and treatment mastitis. The results showed that the average SCC (× 10^4 cells/mL) in the betadine control group (n=20) increased from 30.8 ± 23.3 to 35.6 ± 31.5, while the average SCC (× 10^4 cells/mL) in the Chinese herbs experimental group (n=20) was from 32.2 ± 24.8 to 21.4 ± 18.5. These results showed that the anti- inflammatory herbs dipping agents developed in this experiment had profound significance and development potential in reducing the SCC in raw milk.

1. Chen, JF,Wu, WB,Zhang, MM,Chen, CM(2019).Taraxasterol suppresses inflammation in IL-1β-induced rheumatoid arthritis fibroblast-like synoviocytes and rheumatoid arthritis progression in mice.IntImmunopharmacol,70,274-283.
2. Choi, BR,Cho, I J,Jung, SJ,Kim, J K,Park, S M,Lee, D G,Ku, S K,Park, KM(2020).Lemon balm and dandelion leaf extract synergistically alleviate ethanol-induced hepatotoxicity by enhancing antioxidant and anti-inflammatory activity.J Food Biochem,44(8),e13232.
3. Cobirka, M,ancin, V,Slama, P(2020).Epidemiology and Classification of Mastitis.Animals,10(12),2212.
4. František, Z,Vasil', M,Ondrašovičová, S,Výrostková, J,Bujok, J,Pecka-Kielb, E(2021).Maintaining Optimal Mammary Gland Health and Prevention of Mastitis.Front Vet Sci,8,607311.
5. Ge, BJ,Peng, Zhao,Li, HT,Sang, R,Wang, M,Zhou, HY,Zhang, XM(2021).Taraxacummongolicum protects against Staphylococcus aureus-infected mastitis by exerting anti-inflammatory role via TLR2-NF-κB/MAPKs pathways in mice.J Ethnopharmacol,268,113595.
6. Hu, C.,Kitts, D. D.(2005).Dandelion (Taraxacumofficinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro.Phytomedicine,12(8),588-597.
7. Hu, G,Wang, JJ,Hong, D,Zhang, T,Duan, HQ,Mu, X,Yang, ZJ(2017).Effects of aqueous extracts of TaraxacumOfficinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.BMC Complement Altern Med,17(1),38.
8. Hu, H,Fang, Z,Mu, T,Wang, Z,Ma, Y,Ma, Y(2021).Application of Metabolomics in Diagnosis of Cow Mastitis: A Review.Front Vet Sci,8,747519.
9. Isobe, N,Kurose, T,Suzuki, N,Koshiishi, T,Ueno, K,Hisaeda, K(2022).Effect of oral administration of colostrum on inflammation in the udders of dairy cows suffering from mastitis.J Vet Med Sci,84(1),59-63.
10. Jeon, D,Kim, S J,Kim, H S(2017).Anti-inflammatory evaluation of the methanolic extract of Taraxacumofficinale in LPS-stimulated human umbilical vein endothelial cells.BMC Complement Altern Med,17(1),508.
11. Jeon, HJ,Kang, HJ,Jung, HJ,Kang, YS,Lim, CJ,Kim, YM,Park, EH(2008).Anti-inflammatory activity of Taraxacumofficinale.J Ethnopharmacol,115(1),82-88.
12. Jin, R.,Wan, L. L.,Mitsuishi, T.,Sato, S.,Akuzawa, Y.,Kodama, K.,Kurashige(1994).Effect of Shi-Ka-Ron and Chinese herbs on cytokine production of macrophage in immunocompromised mice.Am. J. Chinese Med,22,255-266.
13. Kamboj, V. P.(2003).Herbal medicine.Current Science,78,35-39.
14. Kuo, Y. C.,Sun, C. M.,Tsai, W. J.,Ou, J. C.,Chen, W. P.,Lin, C. Y.(1999).Blocking of cell proliferation, cytokine production and genes expression following administration of Chinese herbs in the human mesangial cells.Life Sci,64,2089-2099.
15. Lu, XA,Wo, GQ,Li, BH,Xu, C,Wu, JH,Jiang, CQ,Wei, JW(2018).The anti-inflammatory NHE-06 restores antitumor immunity by targeting NF-κB/IL-6/STAT3 signaling in hepatocellular carcinoma.Biomed Pharmacother,102,420-427.
16. Ma, CH,Zhu, LP,Wang, J,He, H,Chang, XY,Gao, J,Shumin, SM,Yan, TH(2015).Anti-inflammatory effects of water extract of Taraxacummongolicumhand.-Mazz on lipopolysaccharide-induced inflammation in acute lung injury by suppressing PI3K/Akt/mTOR signaling pathway.J Ethnopharmacol,168,349-355.
17. Piao, TK,Ma, ZQ,Li, X,Liu, JY(2015).Taraxasterol inhibits IL-1β-induced inflammatory response in human osteoarthritic chondrocytes.Eur J Pharmacol,756,38-42.
18. Ranjani, S,ShruthyPriya, P,Veerasami, M,Hemalatha, S(2022).Novel PolyherbalNanocolloids to Control Bovine Mastitis.ApplBiochemBiotechnol,194(1),246-265.
19. Ren, YS,Zheng, Y,Duan, H,Lei, L,Deng, X,Liu, XQ,Mei, ZN,Deng, XK(2020).Dandelion polyphenols protect against acetaminophen-induced hepatotoxicity in mice via activation of the Nrf-2/HO-1 pathway and inhibition of the JNK signaling pathway.Chin J Nat Med,18(2),103-113.
20. Schütz, K,Carle, R,Schieber, A(2006).Taraxacum--a review on its phytochemical and pharmacological profile.J Ethnopharmacol,107(3),313-323.
21. Seo, S. W.,Koo, H. N.,An, H. J.,Kwon, K. B.,Lim, B. C.,Seo, E. A.,Ryu, D. G.,Moon, G.,Kim, H.Y.,Kim, H. M.,Hong, S.H.(2005).Taraxacumofficinale protects against cholecystokinin-induced acute pancreatitis in rats.World J Gastroenterol,11(4),597-599.
22. Wang, SS,Wang, Y,Liu, XY,Guan, LZ,Yu, LZ,Zhang, XM(2016).Anti-inflammatory and antiarthritic effects of taraxasterol on adjuvant-induced arthritis in rats.J Ethnopharmacol,187,42-48.
23. Wei, N C,Sung, G H(2020).Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments - A review.Asian-Australas J AnimSci,33(11),1699-1713.
24. Wente, N,Grieger, A S,Klocke, D,Paduch, J-H,Zhang, Y,Leimbach, S,ThoSeeth, M,Mansion-De, Vries E M,Mohr, E,Krömker, V(2020).Recurrent mastitis-persistent or new infections?.Vet Microbiol,244,108682.
25. Winkler, M J,Franz, S,Wittek, T,Pothmann, H(2021).A potential treatment approach for subclinical mastitis in dairy cows: auriculotherapy of the auricular branch of the vagus nerve.J Dairy Res,88(4),407-412.
26. Xu, L,Yu, YF,Sang, R,Li, JX,Ge, BG,Zhang, XM(2018).Protective Effects of Taraxasterol against Ethanol-Induced Liver Injury by Regulating CYP2E1/Nrf2/HO-1 and NF- κ B Signaling Pathways in Mice.Oxid Med Cell Longev,2018,8284107.
27. Yang, N,Dong, ZB,Tian, G,Zhu, MM,Li, C,Bu, WQ,Chen, J,Hou, XF,Liu, Y,Wang, G,Jia, XB,Di, LQ,Feng, L(2016).Protective effects of organic acid component from TaraxacummongolicumHand.-Mazz. against LPS-induced inflammation: Regulating the TLR4/IKK/NF-κB signal pathway.J Ethnopharmacol,194,395-402.
28. Yang, N,Li, C,Tian, G,Zhu, MM,Bu, WQ,Chen, J,Hou, XF,Di, LQ,Jia, XB,Dong, ZB,Feng, L(2016).Organic acid component from TaraxacummongolicumHand.-Mazz alleviates inflammatory injury in lipopolysaccharide-induced acute tracheobronchitis of ICR mice through TLR4/NF-κB signaling pathway.IntImmunopharmacol,34,92-100.
29. Zaatout, N(2022).An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies.Microbiol Res,256,126960.
30. Zhang, Y,Hu, YF,Li, W,Xu, GY,Wang, KR,Li, L,Luo, H,Zou, L,Wu, JS(2022).Updates and advances on pharmacological properties of TaraxacummongolicumHand.-Mazz and its potential applications.Food Chem,373(Pt A),131380.
31. Zhao, L,Zhao, JL,Bai, ZH,Du, JH,Shi, YC,Wang, Y,Wang, YY,Liu, YZ,Yu, Z,Li, MY(2022).Polysaccharide from dandelion enriched nutritional composition, antioxidant capacity, and inhibited bioaccumulation and inflammation in Channaasiatica under hexavalent chromium exposure.Int J BiolMacromol,201,557-568.
32. 王詩茜,何偉杰,何俊賢,李彥霖,郭宗甫(2009)。蒲公英的藥用功效與生活應用。中華傳統獸醫學會會刊，13(2)，12-22。
33. 行政院衛生署中華藥典中藥集編修小組(2004).中華中藥典.台北市:行政院衛生署.
34. 吳永惠(2002).牛病學.台北市:藝軒圖書出版社.
35. 吳義興,陳素貞,蕭終融,張惟茗(1987)。牛乳房炎乳汁中病原細菌分離鑑定及其在周圍環境之分布。台灣省家畜衛生試所研究報告，23，7-11。
36. 李國華(2013)。國立臺灣大學臨床動物醫學研究所。
37. 李國華,陳志毅,葉家舟,王思涵,張菊犁,賈玉祥,季昭華(2016)。蒲公英對次臨床性乳房炎乳牛之生乳成分、體細胞數、間白素-8、血球及血清生化性狀之影響。畜產研究，49(3)，201-208。
38. 陳書凱,陳鵬文,莊士德(2008)。台灣乳牛分娩後疾病之調查。台灣獸醫誌，34，41-48。
39. 陳煥南,李素珍,毛嘉洪,徐慶霖(1992).乳房炎全面還擊.台北市:台灣區雜糧發展基金會.
40. 羅仁,秦建增(2006).單味中藥療法.北京市:人民軍醫出版社.