聚乙烯醇/竹炭奈米粒子複合水凝膠材料 之製備及性質研究

Pol(vinyl Alcohol)/Bamboo Charcoal Nanoparticle Composite Hydrogel： Preparation and Characterization

10.6834/CSMU.2011.00174

田奕紘

聚乙烯醇 ； 竹炭奈米粒子 ； 奈米複合水凝膠 ； 氫氧基磷灰石 ； Polyvinyl alcohol ； Bamboo charcoal nanoparticles

中山醫學大學口腔材料科學研究所學位論文

2011年

碩士

湯正明

繁體中文

聚乙烯醇具有良好的親水性、化學穩定性和生物相容性，廣泛的被應 用於生物醫學領域，如藥物輸送、傷口敷料、生物支架。竹炭基於其多孔 特性，具有良好的吸附性，本身具有鉀、鈉、鈣、鎂等礦物質，可溶解在 水中。本研究選擇在聚乙烯醇水溶液中添加不同比例的奈米化竹炭製成的 水凝膠複合膜材為實驗組，純聚乙烯醇膜材為對照組，以萬能拉力機進行 拉伸測試，由機械性質分析可看出添加5%竹炭奈米粒子的乾燥複合膜材， 其拉伸強度顯著大於聚乙烯醇及其他複合水膠膜，然而在濕潤狀態下，由 於分子鏈間距離加大，因此需要提高竹炭粒子的濃度至20%，其拉伸強度 才有顯著的影響。以XRD 分析其晶體結構沒有明顯的變化，僅聚乙烯醇 的繞射峰比值有顯著的變化，代表聚乙烯醇分子鏈在水合狀態下會受竹炭 奈米粒子影響，在結晶方向產生變化。在生物活性測試，以模擬體液分別 浸泡一星期後，可發現添加0.1%竹炭奈米粒子之複合水凝膠膜材(PB0.1) 表面即產生礦物沉積，以XRD 分析其結構確定為氫氧基磷灰石，其中以 添加5%竹炭奈米粒子之複合水凝膠(PB5)最為顯著，推測與水凝膠表面親 疏水性微分布有所關聯。未來將繼續探討對於軟骨與硬骨細胞貼附、生長 與分化造成之影響，以瞭解此材料在組織工程支架以及導引組織再生膜材 的應用潛力。

Polyvinyl alcohol (PVA) has good hydrophilicity, chemical stability and biocompatibility, are widely used in biomedical applications, such as drug delivery, wound dressings, scaffolds. In the other hand, porous bamboo charcoal has a good gas adsorption. Bamboo charcoal containing water-soluble minerals, such as potassium, sodium, calcium, magnesium. In this study, bamboo charcoal nanoparticles (BCNP) was added into PVA aqueous solution. Composite membranes were prepared by solvent casting and mechanical properties were characterized by universal testing machine. The result shows that tensile strength of dry composite membrane contains 5% BCNP (PB5) significantly greater than PVA and other PVA/BCNP membrane. However, the mechanical property was significantly changed of composite membrane in hydrogel state, PB20 membrane greater than PVA and other composite membranes. Perhaps the distance between PVA molecule chains is increased, more BCNP to maintain its structure. The crystal structure of membrane was analyzed by X-ray diffraction (XRD). Only the high ratio of PVA diffraction peak have a significant change, on behalf of PVA molecular chains in the hydrated state will be affected by BCNP impact, then crystal orientation was change. The bioactivity of membrane was evaluated by immersion in simulated body fluid (SBF) one week later. Composite membrane surface have mineral deposition and PB5 is significant then other sample. The mineral by XRD identified is hydroxyapatite (HA). Suggesting affinities with the hydrogel membrane surface has been associated with hydrophilic/hydrophobic micro-distribution. The future will continue to evaluate for adhesion, proliferation and differention of chondrocyte and osteoblast cells in composite.

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