聚乙二胺樹枝狀高分子衍生物之製備及與哆醛結合能力之研究

The Preparation and Characterization of Peptide Conjugated Polyamidoamine(PAMAM) Dendrimer

10.6832/KMU.2008.00138

謝光展

樹枝狀高分子 ； 胜肽液相合成 ； dendrimer ； peptide ； liquid-phase synthesis

高雄醫學大學醫藥暨應用化學研究所學位論文

2008年

碩士

高佳麟

繁體中文

為達到結合哆醛-5-磷酸鹽(pyridoxal-5-phosphate， PLP)的目的，我們在聚乙二胺樹枝狀高分子的尾端修飾數種含有離胺酸不同序列的短鏈胜肽。目前，我們成功的合成出化合物14, 39, 41，化合物14與39分別為第三代與第四代聚乙二胺樹枝狀高分子衍生之產物，觀察其與PLP鍵結之能力，以了解代數上的差異性。41則為具有酪胺酸的胜肽序列，以瞭解酪胺酸的影響。合成之化合物與PLP的結合能力，則經由Scatchard-Klotz方法分析，以得到結合常數與數目。14與PLP的結合常數為0.35 mM-1，已經達到天然酵素與PLP結合能力的範圍。因此對於其他化合物，我們有其他有更佳的結合常數之胜肽樹枝狀高分子，此一結果可以證實我們的想法是合理且可行的。

This project focus on the design, preparation and characterization of carrier macromolecular which are able to carry pyridoxal-5-phosphate (PLP). We had synthesized several modified PAMAM dendrimers. With synthetic short peptide containing lysine, we introduce those peptides on the surface of G3 and G4 PAMAM dendrimer. As far, compounds 14, 39 and 41 were successfully prepared under linear assembly approach. Compounds 14 and 39 are derivatives of G3 and G4 PAMAM dendrimer, respectively. To learn the binding affinity of synthetic dendrimer, the Scatchard-Klotz analysis was applied. The result shows the binding constant of our synthetic molecle up to 0.35 mM-1 which is similar to the known natural PLP associate enzymes. Meanwhile, the compound 41, tyrosine was used instead of phenylalanine, to reveal the possible capality of tyrosine. The results suggest our hypothesis should be reasonable and feasible.