苦蘵有效成分化學製備與分析

Chemical Preparation and Analyses of Active Principles from Physalis angulata

柯泓瑋

苦蘵 ； Physalin ； Physalis angulata ； Physalin

高雄醫學大學天然藥物研究所碩士班學位論文

2017年

碩士

張芳榮

繁體中文

本研究探討從不同溶媒萃取方式，建立苦蘵活性指標化合物 physalins產率最佳化之萃取及製程。利用最有效的溶媒萃取、濃縮、快速分離，進一步研究萃取物中四種活性指標成分physalin B、physalin C、physalin F與physalin U之定性、定量評估，及探討在不同保存條件下化合物physalins成分之安定性。此研究提供一個具代表性的化學指紋圖譜、建立苦蘵活性成分品質管理平台，及其有效的保存方法，在未來可應用於苦蘵相關產品之管控。 透過核磁共振(NMR)光譜數據分析以及比對文獻上四個已知physalins，並將四個化合物分別進行A549、MDA-MB-231和HepG2人類細胞株之細胞毒殺篩選。其中化合物physalin F (3)對上述細胞株最具有細胞毒殺作用(IC50 = 0.56‒0.67 μg/mL) 。

In this investigation, we report a convenient method for concentrating the physalin-rich fraction, from different kinds of the solvent extraction design on Physalis angulata. The concentrate of four major active ingredients in optimization preparation was identified. Efficient extraction, concentration, and rapid analyses methods provide necessary information for quality control assessment of physalins B, physalin C, physalin F, and physalin U. In addition, the stability for qualitative changes of the components is further explored for the advance management for mass production of this plant material. The structures of the isolated compounds were elucidated by the analyses of NMR spectral data. The four major components 1−4 of P. angulata were tested for their cytotoxicity against A549, MDA-MB-231, and HepG2 cell lines. Among all tested compounds, physalin F (3) is the most potent compound with the IC50 values ranged from 0.56 to 0.67 μg/mL against aforementioned cancer cell lines.

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