蝦紅素在高脂飲食所誘導出類憂鬱行為小鼠之效果

Effect of Astaxanthin in high fat diet induced depressive-like mice

李忠霖

蝦紅素 ； 高脂飲食 ； 尾部懸吊實驗 ； 強迫游泳實驗 ； 明暗箱實驗 ； 類憂鬱行為 ； astaxanthin ； high fat diet ； tail suspension test ； forced swimming test ； light-dark box ； depressive-like behavior

長榮大學醫學研究所學位論文

2016年

碩士

紀宗呈

繁體中文

憂鬱症是一種很常見的心理疾病，而且也有研究指出憂鬱症、 高脂飲食和發炎物質有關連性。目前已經有研究指出高脂飲食會誘 導出小鼠的類憂鬱行為，所以本研究的目的是利用高脂飲食誘導出 小鼠的類憂鬱行為，再餵食具有抗氧化作用的蝦紅素，觀察是否能 抑制小鼠的類憂鬱行為。在實驗設計上分為控制組、高脂飲食加上餵食生理食鹽水（0.4mg/day）、高脂飲食加上餵食低劑量的蝦紅素（0.4mg/day）、高 脂飲食加上餵食中劑量的蝦紅素（0.8mg/day）和高脂飲食加上餵食 高劑量的蝦紅素（1.6mg/day）；進一步觀察小鼠在行為實驗（尾部 懸吊實驗、強迫游泳實驗、明暗箱實驗）中蝦紅素是否能改善類憂 鬱行為。 實驗結果顯示，在三十天的實驗後，高脂飲食加上餵食生理食 鹽水（0.4mg/day） 的小鼠能誘導出類憂鬱行為； 而餵食蝦紅素 0.4mg/day、0.8mg/day 和 1.6mg/day 劑量下，隨著劑量相關性的增加， 改善類憂鬱行為的效果也隨之增加。本研究證實食用高脂飲食加上生理食鹽水的小鼠會誘導出類憂 鬱行為，並且在蝦紅素治療後確實有改善小鼠類憂鬱行為的功能。

Depression is a very common mental illness. Some research points out cause of this disease is associate with foods which are high in both fat and sugar. Recent research already proves that high fat diet would induce depressive-like behavior in mice. Hence, the purpose of our research is inducing depressive-like behavior in mice by using high fat diet, and then feeding them with antioxidant astaxanthin, and then observing if depressive-like behavior in mice can be controlled. Design of experiments is divided into control team, feeding with high fat diet plus normal saline (0.4mg/day), feeding with high fat diet plus low- dose astaxanthin (0.4mg/day), feeding with high fat diet plus mid-dose astaxanthin (0.8mg/day), feeding with high fat diet plus high-dose astaxanthin (1.6mg/day); Furthermore, observing if use of astaxanthin in behavioral experiments (tail suspension test, forced swimming test, light- dark box) could change depressive-like behavior in mice. Experimental result shows that, after 30-day experiment, feeding with high fat diet plus normal saline (0.4mg/day) would induce depressive-like behavior in mice; and, feeding with astaxanthin 0.4mg/day, 0.8mg/day, and 1.6mg/day dosage, as daily dosage increases, would also mitigate depressive-like behavior. This research proves that, feeding mice with high fat diet plus normal saline would induce depressive-like behavior, and feeding with astaxanthin would help mitigate depressive-like behavior in mice.

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