研究雙離子型β-羥基丁酸酯纖維薄膜的組織相容性控制

A Study of Zwitterionic Polyhydroxybutyrate Fibrous Membranes with Controlled Cytocompatibility

10.6840/cycu201600320

許豐發

β-羥基丁酸酯 ； 雙離子 ； PHB ； zP(4VP-r-ODA) ； electrospinning ； antifouling membranes ； tissue engineering scaffold

中原大學化學工程學系學位論文

2016年

碩士

張雍;費安東

英文

於此研究中，電紡絲程序被採用來製備聚羥基丁酸酯(PHB)纖維狀薄膜，並以物理塗布聚(四-乙烯吡啶-丙烯酸十八酯) (zP(4VP-r-ODA))之雙離子型共聚高分子來強化其纖維表面抵抗生物結垢物質的污染或沾粘特性，而塗布量範圍控制在0到2.5 mg/cm2。由電子顯微鏡的膜結構顯示表面孔隙度會隨高分子塗布量的提升而降低，這也可以由膜孔隙度量測來驗證支持。雙離子型共聚高分子批覆於纖維表面的比例可進一步由傅立葉轉換紅外線光譜與X射現光电子能譜的量測來鑒定，其中量化比例可由 zP(4VP-r-ODA)的四級胺基團在402 eV的電子結合能來進行定量分析。由共聚物的塗布可以顯著的降低膜面的水接觸角(由130°到80°)與提高電紡絲纖維的水合量，所形成的水合保護層可以產生非常低的蛋白質吸附。在微觀尺度下，控制共聚物於PHB纖維薄膜最佳化的塗布量0.17 mg/cm2條件下，其牛血清白蛋白吸附量約0.01 mg/cm2。類似的結果也可於溶菌酶與纖維蛋白原這兩種蛋白質中觀察到。在巨觀尺度下，細菌(大腸桿菌)與血球(紅血球、白血球、血小板、全血)貼附可有效降低當共聚物的塗布量0.5 mg/cm2以上。相同的趨勢結果也可在成體纖維母細胞的貼附與生長實驗中觀察到。由本研究成果指出zP(4VP-r-ODA)塗布改質之PHB纖維薄膜可用於組織工程支架來控制細胞生長與組織再生。

In this work, electrospinning process was used to form poly(3-hydroxybutyric acid) (PHB) fibrous membranes. Fibers were then coated with zwitterionic poly(4-vinylpyridine-random-octadecylacrylate) zP(4VP-r-ODA), in order to enhance the resistance of membranes to biofouling. Coating density was in the range 0-2.5 mg/cm2. The structure was characterized by SEM, which showed a significant decreasing of surface porosity with coating density, also supported by the assessment of surface porosity. The presence of zwitterionic polymer onto the fibers was further evidenced by FT-IR and XPS measurements. The later revealed the presence of a peak at a binding energy of 402 eV, ascribed to the quaternary amine of zP(4VP-r-ODA). The copolymer also enabled to importantly decrease the water contact angle (from 130° to 80°) and increase the hydration capacity of electrospun fibers. The formation of a hydration protective layer resulted in very low level of protein adsorption, as it was measured to be around 0.01 mg/cm2 for membrane with the highest coating density, while it was 0.17 mg/cm2 for virgin PHB membrane. Similar results were obtained with lysozyme and fibrinogen proteins. At a microscale, the adhesion of bacteria (Escherichia coli) and that of blood cells – erythrocytes, leukocytes, platelets and whole blood – was drastically reduced for coating density as low as 0.5 mg/cm2. A similar trend was observed with fibroblasts. Taken all together, these results suggests that the zP(4VP-r-ODA) coated PHB fibers could be used as tissue engineering scaffold to control the cell growth and tissue regeneration.

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