以紫外光調製耐用型金量子簇並用於生物標記研究

UV-Tunable and Durable Gold Quantum Clusters for Biological Labeling

10.6840/cycu201500908

林佳慧

金量子簇 ； 光還原反應 ； 光譜調控 ； 磷脂質 ； Gold quantum clusters ； Tunable fluorescent wavelength ； Photoreduction

中原大學生物醫學工程學系學位論文

2015年

碩士

林政鞍

繁體中文

近年來在奈米科技領域中，螢光奈米探針廣泛應用於生醫領域上，傳統以有機螢光染劑、量子點及螢光金屬奈米團簇為主，在生物標定與造影成像極為重要。其中量子點具有螢光波長可調、高量子產率、低光漂白，但仍在毒性上有使用限制；而貴金屬團簇具有物理尺寸小、螢光可調及生物相容性，但需添加表面配體分子進行保護。本研究結合了量子點螢光波長可調、低光漂白以及貴金屬團簇物理尺寸小、生物相容性之優點，提出金量子簇，利用一簡易的方法製備螢光金量子簇，並首次提出以光還原反應來調控螢光光譜，深入探討光還原對於金量子簇的光學特性及結構等分析。其次，利用界面活性劑增強藍色金量子簇光學特性並藉由光還原來調控螢光特性。最後，使用磷脂質將有機相金量子簇成功改質至水相，並且保有其原本的螢光特性，可以使這種合成方法簡單的有機相金量子簇未來可以廣泛應用於廣泛應用在生醫標定、細胞追蹤、分子檢測等領域。

Fluorescent probes, like Organic fluorescent dyes, fluorescent quantum dots and metal nanoclusters are widely used in nanotechnology biomedical field in recent years, mostly in contrast imaging and tracking. Quantum dots advantages are the fluorescent wavelength is accustomed, high quantum yield and low photobleaching effect, but there are restrictions of toxicity. On the other side, metal nanoclusters have small size, tunable fluorescent wavelength and biocompatibility, but require addition of surface ligand as the protection. In this study, gold quantum clusters combining the advantages of quantum dots which is with tunable fluorescence wavelength and low photobleaching and metal nanoclusters which is with small size and biocompatible is proposed. Utilizing a simple method to synthesize gold quantum clusters and proposing tunable fluorescence wavelength according to corresponding reaction of photon reduction deeply explore gold quantum clusters reacting to photon reduction and further analyze the optical properties and structure. Secondly, different surfactants can enhance and regulate optical properties of blue gold quantum clusters by photon reduction. Finally, the hydrophobic of gold quantum clusters can be transferred easily to hydrophilic phase through lipid micelle while retaining its original fluorescent properties. The experiment will be very useful for gold quantum clusters synthesis and their fluorescence control, moreover this method is environmental friendly, thus can be developed to improve our human health in the future.

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