不同條件下對小球藻生長與油脂累積之探討

investigation of the growth and lipid accumulation between different conditions of chlorella

10.6840/cycu201200768

張哲瑋

微藻類 ； 油脂產率 ； 光照 ； 培養基 ； 混營 ； mixotrophy ； Microalgae ； Lipid productivity ； Light intensities ； Medium

中原大學土木工程學系學位論文

2012年

碩士

黃郁慈;游勝傑

繁體中文

微藻具有快速生長以及能經由光合作用將無機碳轉化成生質能，或利用有機碳直接攝取並轉化成生質能等優點，且其油脂與柴油成分相近，但不會產生硫化物等汙染物質，固以培養微藻作為生質能源具有相當的發展潛力。 本研究主要研究對象為有快速生長其特性，以及文獻中提及到油脂量介於5%¬¬－50%之間之淡水藻Chlorella vulgaris。將其藻種活化養後利用批次實驗培養於不同的光照強度(4,000lux、8,000lux、15,000lux)以及LE medium培養基、人工廢水(modified Y. Feng et al., 2011)，並以光學密度、藻細胞數、藻體乾重、葉綠素a含量及脂質含量與不同天數時作分析，找出最佳生長及油脂含量條件。 研究結果顯示，LE medium培養基下，8,000lux光照下培養7天為油脂產量及產率之最佳條件，其油脂含量及產率分別為38.45%、78.0±4.16 mg/L/d，平均生長速率(μnet)為0.31d-1。高光照下(15.000lux)不管是培養4天或7天都會抑制其油脂含量與產率。低光照(4,000lux)下的油脂含量第4天和第7天都無太大差別。在人工廢水培養基下，15,000lux下培養7天為油脂產量及產率之最佳條件，其油脂產量及產率分別325.8±56.4 mg/l、46.5±8.1mg/l/d。

Microalgae has considerable potential as a source of biodiesel because of its attractive properties, i.e. fast growth, ability to use inorganic carbon by photosynthesis, transfer organic carbon to biomass and lipid products are similar to diesel, but does not produce sulfides pollutions. In this study, the main object of study, Chlorella vulgaris, has characteristics of fast growth, and high lipid-containaing (5%¬¬ to 50% of biomass). It was cultured by batch under various light intensities(4000, 8000,15000lux) and medium(LE medium, Artificial wastewater modified Y. Feng et al., 2011). The optical density, number of algal cell, cell weight, chlorophyll-a, and lipid content were measured to identify the condition that maximized biomass production and lipid accumulation at various incubation times. Results demonstrate that 6000lux and 7 days incubation in LE medium yield the highest lipid concentration and lipid production rate (38.45%, 78.0±4.16 mg/L/d), average biomass growth rate μnet was 0.31d-1. Whether cultured by 15000lux, it was incubated for 4 days or 7 days will inhibit lipid concentration and lipid production rate. Algal was cultured 4 days or 7 day under 4000 lux of lipid concentration was no big difference. And 6000lux and 7 days incubation in Artificial wastewater (modified Y. Feng et al., 2011) yield the highest lipid yield and lipid production rate (325.8±56.4 mg/l、46.5±8.1mg/l/d).

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