以金屬有機骨架-高分子整體材料為固相微萃取之吸附劑萃取磺胺類抗生素與非類固醇抗發炎藥物

Solid-Phase Microextraction of Sulfonamide Antibiotics and Non-Steroid Anti-Inflammatory Drugs using Metal-Organic Framework Polymeric Monoliths as Absorbent

10.6840/cycu201400966

王琨允

固相微萃取 ； 金屬有機骨架-高分子 ； 非類固醇藥物 ； 磺胺類 ； metal-organic framework–polymer ； solid-phase microextraction ； sulfonamides ； non-steroid anti-inflammatory drugs

中原大學化學系學位論文

2014年

碩士

黃悉雅

繁體中文

本論文以金屬有機骨架-高分子(metal-organic framework–polymeric monolith, MOF-polymer)整體成形材料做為固相微萃取(solid-phase microextraction, SPME) 之吸附劑，進行磺胺類 (sulfonamides) 抗生素及非類固醇藥物 (non-steroid anti-inflammatory drugs, NSAIDs) 萃取。 第一部份探討MOF-polymer進行磺胺類藥物之SPME萃取，並以微乳化電層析 (microemulsion electrokinetic chromatography, MEEKC) 方法檢測萃取效果。實驗中探討不同型態MOF對磺胺類藥物之萃取影響，並進行最佳化條件測試。結果顯示在50 wt% MIL-53(Al)含量、pH 5樣品基質、5 cm萃取管柱長、1 mL MeOH脫附溶劑與0.125 mL/min萃取流速為最佳化條件，得到磺胺類藥物之萃取回收率為40.6% - 93.0%，相對標準偏差 (relative standard deviation, RSD) 低於4.8% (intra-day)；不同天萃取回收率為40.6% - 91.2%，RSD低於6.4% (inter-day)；不同合成批次之萃取管柱回收率為41.9% - 89.7%，RSD低於5.3% (column-to-column)，此MIL-53(Al)-polymer可重複使用至少30次，顯示具有良好管柱再現性。 第二部份探討MOF-polymer對非類固醇藥物之萃取效果，以33%的MIL-101(Cr)含量、0.125 mL/min萃取流速為最佳化條件，得到之回收率為93.1% - 106.1%，RSD低於5.7%；並比較不同種類MOFs萃取效果，DUT-5回收率為91.0% - 106.6%，RSD低於8.7%； MIL-100(Fe)回收率為52.1% - 79.6%，RSD低於10.5%； UiO-66回收率為46.7% - 69.9%，RSD低於10.4%，結果證明MOF-polymer可成功應用於非類固醇藥物之SPME萃取。

In this study, metal-organic framework–polymeric monolith (MOF-polymer) were used as adsorbent for solid-phase microextraction (SPME) of sulfonamides and non-steroidal anti-inflammatory drugs (NSAIDs). For the first part in this dissertation, MOF-polymers were applied for SPME technology for sulfonamides extraction and analyzed by using microemulsion electrokinetic chromatography (MEEKC) method to determine the extraction efficiency. Considering the extraction efficiency of sulfonamides, different type of MOFs and extraction conditions were optimized. The results show that 50 weight percent MOF amount, sample matrix at pH 5, 5 cm of extraction column lengths, 0.125 mL/min of extraction flow rate and 1 mL methanol as eluting solvent for optimized conditions. Based on the optimized results, the recoveries and relative standard deviation (RSD) for intra-day were ranged from 40.6% - 93.0% and <4.8%, respectively. For inter-day, the recoveries were ranged from 40.6% - 91.2%, and RSD < 6.4 %. In addition, the recoveries of column-to-column were ranged from 41.9% - 89.7% and RSD < 5.3%, demonstrated MIL-53(Al)-polymer column was high reproducibility. Furthermore, the MIL-53(Al)-polymer column can be re-used at least 30 times. For the second part in this dissertation, MOF-polymers were applied for SPME technology for NSAIDs extraction. The results show that 33 weight percent MOF amount, 0.125 mL/min of extraction flow rate for optimized conditions. Based on the optimized results, the extraction recoveries by using MIL-101(Cr)-polymer were ranged from 84.0 % - 104.5 % and RSD < 5.7%.Comparison with different type of MOF for NSAIDs extraction, the extraction recoveries by using DUT-5-polymer were ranged from 91.0% - 106.6% and RSD < 8.7 %. The extraction recoveries by using MIL-100(Fe)-polymer were ranged from 52.1% - 79.6% and RSD < 10.5%. The extraction recoveries by using UIO-66-polymer were ranged from 46.7% - 69.9% and RSD < 10.4%.Finally, the results showed that the MOF-polymer were successfully applied for SPME application in the extraction of NASIDs.

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