純種菌株去除人工合成廢水中甲胺之批次及連續流實驗研究

Methylamine Removal from Synthetic Wastewater Using Pure Strain in Batch Reactor and a Continuous Stirred Tank Reactor

王俊欽(Chun-Chin Wang)；曾俊賢(Chun-Hsien Tseng)；溫志銘(Chih-Ming Wen)

甲胺 ； 生物分解 ； 批次實驗 ； 連續流實驗 ； batch reactors ； biodegradation ； continuous stirred-tank reactor (CSTR) ； methylamine (MA)

先進工程學刊

8卷4期（2013 / 10 / 01）

229 - 233

繁體中文

甲胺為惡臭物質，溢散至大氣中可能會造成空氣污染；排放至土壤或水體中可能會造成土壤及水體污染。為避免使用過程中造成生態影響及環境傷害，因此本研究希望重新從環境中獲得可去除甲胺之純種菌株，亦即從都市、工業區污水處理廠及水稻田中獲得污泥或土壤，將該污泥或土壤混合後以甲胺馴養，冀望能從經馴養之混合菌群中分離及篩選出可以去除甲胺的純種菌株，並藉由批次實驗與連續流試驗瞭解經篩選獲得之甲胺分解菌對人工合成廢水中甲胺的利用情況。結果顯示，經化學恆定反應槽利用甲胺培養之混合菌株以甲胺培養基篩選獲得的5株菌株均不能利用甲胺生長，但以R2A培養基篩選獲得之4株菌株中以編號MRA1對甲胺之去除效果最佳。菌株MRA1經16s rRNA序列菌種鑑定後所得之菌名與菌株Acinetobacter calcoaceticus strain L22相似。批次條件下，菌株Acinetobacter calcoaceticus MRA1可利用人工合成廢水中1000 mg/l以下之甲胺生長，且對1000 mg/l以下之甲胺有100％之去除率。當懸浮生長式連續流反應槽之水力停留時間為1.136天時，菌株Acinetobacter calcoaceticus MRA1對甲胺之去除效果最佳，且對1000 mg/l以下之甲胺有100％之去除率。

Methylamine (MA) has strong odor similar to fish and can cause chemical burns to the eyes and skin. Besides, indiscriminate discharging of contaminated MA wastewater or wastes into natural water will cause serious environment pollution and has severe impact on the aquatic ecosystem. Thus, this study aims to isolate and identify the pure utilizing bacteria from the environment to remove MA and also to understand the ability of acclimatized bacteria from synthetic wastewater in the batch reactor and in the continuous stirred-tank reactor (CSTR). The results show five strains were isolated from the MA medium plate and they couldn't utilize MA to grow. Four strains were isolated from the YE medium plate and one of them had their better MA removing efficiency. Meanwhile, the MA removing utilizing strain bacteria was identified as similar to Acinetobacter calcoaceticus strain L22 by the method of 16S rDNA gene sequence. And strain Acinetobacter calcoaceticus MRA1 could utilize MA from synthetic wastewater up to 1000 mg/l as a substrate to grow in the batch reactor and the removing efficiency of MA reached 100%. Moreover, when hydraulic retention time (HRT) was 1.136 day, the MA removing efficiency in the CSTR reach its best performance. That is, strain Acinetobacter calcoaceticus MRA1 could utilize 1000 mg/l MA from synthetic wastewater to grow in the CSTR as well as the MA removing efficiency reached 100%.

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