評估pCpG ODN 1668 對海鱺免疫之影響

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评估pCpG ODN 1668 对海鲡免疫之影响

Evaluation of pCpG ODN 1668 effect on immunal response on Cobia

方季 , 硕士　　导师：郑达智　　

英文

CpG ODN 1668 ；不活化细菌；佐剂；海鲡； CpG ODN 1668 ； inactivated vaccine ； adjuvant ； cobia

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摘要 │ 参考文献 (114) │ 国际计量

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Synthetic unmethylated cytosine phosphorothioate guanine oligodeoxynucleotides (CpG ODNs) which mimics bacterial DNA is one of the options that can be used as an immunostimulant and an adjuvant in inactivated vaccines. They activate both the innate and adaptive immune responses in vertebrate organisms. The B class of CpG ODN 1668 was used in the study to evaluate its effects on the immune response in juvenile cobia fish when used as a plasmid on vaccine. In the study juvenile cobia approximately 25 cm weighing 30 grams were injected with formalin killed bacteria (FKB), pSB1C3 50 µg, pSB1C3 100 µg, pSB1C3 50 µg + FKB, pSB1C3 100 µg + FKB, pCpG 1668 50 µg + FKB and pCpG 1668 100 µg + FKB. Blood serum from cobia was extracted for the analysis of lysozyme, peroxidase and myeloperoxidase activities at 1, 3, 6, 14, 20 and 28 days .The liver, spleen and intestines were sampled for the evaluation of Toll like receptor 9 (TLR 9) and Interleukin 1 Beta (IL- 1β) gene expression at 1, 5 and 10 days. Histopathology analysis of tissues was assessed to check any
histology changes after vaccination. The lysozyme activity was significantly increased at 6 dpi after vaccinating with pCpG 1668 (50) + FKB. Myeloperoxidase and peroxidase activities were not increased after vaccinating with pCpG 1668 + FKB at (50) and (100). The expression of TLR9 was significantly increased at 1 dpi in spleen after vaccinating with pCpG 1668 (50) + FKB. In the liver, a significant expression of TLR 9 was observed at 1 dpi and 5 dpi vaccinated with pCpG 1668 (100) + FKB and in intestines a significant expression was at 1 dpi vaccinated with pCpG 1668 (100) + FKB. The expression of IL-1β was significantly increased at 1dpi and 10 dpi in all tissues after vaccinating with pCpG 1668 (100) + FKB. There was no or mild histological change observed in pCpG 1668 (100) + FKB and pCpG 1668 (50) + FKB treatments in the liver and spleen. In conclusion pCpG 1668 can increase the expression of TLR 9 and IL-1β and also increase the level of humoral response and has no or mild histological change in cobia.

参考文献 ( 114 ) 〈TOP〉

Agarwal, S. K., F. K. Tan, and F. C. Arnett. 2008. "Genetics and Genomic Studies in Scleroderma (Systemic Sclerosis)," Rheumatic Disease Clinics of North America, 34(1): 17-40.
连结：
Ahmad‐Nejad, P., H. Häcker, M. Rutz, S. Bauer, R. M. Vabulas, and H. Wagner. 2002. "Bacterial Cpg‐DNA and Lipopolysaccharides Activate Toll‐Like Receptors at Distinct Cellular Compartments," European journal of immunology, 32(7): 1958-1968.
连结：
Amanzada, A., I. A. Malik, M. Nischwitz, S. Sultan, N. Naz, and G. Ramadori. 2011. "Myeloperoxidase and Elastase Are Only Expressed by Neutrophils in Normal and in Inflammed Liver," Histochemistry and Cell Biology, 135(3): 305-315. doi: 10.1007/s00418-011-0787-1.
连结：
Anbarasu, K., K. Thangakrishnan, B. Aruna, and M. Chandran. 1998. "Assessment of Immune Response in Freshwater Catfish Mystus Vittatus (Bloch) to Different Bacterins of Aeromonas Hydrophila," Indian journal of experimental biology, 36(10): 990-995.
连结：
Andreoni, F. and M. Magnani. 2014. "Photobacteriosis: Prevention and Diagnosis," Journal of immunology research, 2014.
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使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。