探討腺苷對人類慢性骨髓癌細胞K562的抗癌機制

陳怡吟

山西大学，您好！

感谢采购，人社期刊库｜人社论文库，期间：2025/01/01~2025/12/31

感谢试用，科学期刊库｜科学论文库，期间：2024/10/20~2025/01/20

浏览高级检索

关闭
检索检索高级检索出版物检索
浏览期刊主题浏览核心期刊浏览期刊拼音浏览

关闭

查询符号(半角)	查询符号意义说明
空格	表示为「AND」两个查询词之交集
双引号 ( " " )	片语以双引号标示开始及结束，而且只寻找出现顺序相同的字词，例： " image process "
?	表示一个字母切截，输入两个?表两个字母，依此类推，例：输入「Appl?」，查得结果应为appl e , appl y … ( 常用于英文字查询 )
*	表示不限字母切截，由0~n. 例：输入「appl*」，查得结果应为appl e , appl es , appl y , appl ied , appl ication … ( 常用于英文字查询 )
AND、OR、NOT	布尔逻辑组合关键字，用来扩大或缩小查询范围的技巧。 (1) AND ：缩小查询范围 (2) OR ：扩大查询范围(3) NOT：排除不相关的范围

DOI 是数位物件识别码（ D igital O bject I dentifier ）的简称，
为物件在网路上的唯一识别码，可用于永久连结并引用目标物件。

使用DOI作为永久连结

每个DOI号前面加上「 http://dx.doi.org/ 」便成为永久网址。
如以DOI号为 10.5297/ser.1201.002 的文献为例，此文献的永久连结便是： http://dx.doi.org/ 10.5297/ser.1201.002 。
日后不论出版单位如何更动此文献位置，永久连结所指向的位置皆会即时更新，不再错失重要的研究。

引用含有DOI的文献

有DOI的文献在引用时皆应同时引用DOI。若使用APA、Chicago以外未规范DOI的引用格式，可引用DOI永久连结。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。

书目管理工具

相关连结

问题回报

全文下载

探讨腺苷对人类慢性骨髓癌细胞K562的抗癌机制

Investigating anti-tumoral activity of adenine on human chronic myelogenous leukemia cell K-562

陈怡吟 , 硕士　　导师：高绍轩　　

繁体中文

腺苷人类慢性骨髓癌细胞； K562 ； adenine ； human chronic myelogenous leukemia ； K-562

0 个人觉得这篇文章赞

摘要 │ 参考文献 (54) │ 国际计量

摘要〈TOP〉

并列摘要〈TOP〉

Plant-derived phytochemicals have been used as therapeutic agents for hundreds of years. Natural products from plants and other species have been proven to be a promising resource for mining potential anticancer drugs or compounds. Previous studies have indicated that AMP-activated protein kinase (AMPK) is a key player in maintaining energy homeostasis in response to metabolic stress and activation of AMPK suppresses cell proliferation of non-malignant cells and tumor cells. The aim of this study was to investigate effects of adenine, an active compound isolated from Phyllostachys Edulis leaf extract, on human chronic myelogenous leukemia cell line K562 and further elucidate the underlying mechanisms. Cell viability analysis showed that adenine dose-dependently reduced viability of K562 by using MTT assay. Morphological analysis showed that cell morphology of K562 cells treated with adenine was significantly altered. Flow cytometric analysis showed that adenine obviously induced G2/M phase arrest as well as slightly increased of sub-G1 after 48 hours. Further immunoblotting showed that expression of apoptosis-related proteins are insignificantly influenced. In addition, adenine increased the autophagy marker by using AVO staining. The involvement of AMPK activation in the adenine-induced cellular responses was also demonstrated by using AMPK inhibitor compound c. In conclusion, our findings indicate that viability suppressive effect of adenine on K562 cells may attribute to induction of G2/M arrest and autophagy via activation of AMPK. It suggests that adenine should be beneficial to chronic myelogenous leukemia treatment.

参考文献 ( 54 ) 〈TOP〉

3. Zhang, L., Sankaran, V. G., and Lodish, H. F. (2012) MicroRNAs in erythroid and megakaryocytic differentiation and megakaryocyte-erythroid progenitor lineage commitment. Leukemia 26, 2310-2316
连结：
4. Adams, D. H., and Lloyd, A. R. (1997) Chemokines: leucocyte recruitment and activation cytokines. Lancet 349, 490-495
连结：
5. Ketley, N. J., and Newland, A. C. (1997) Haemopoietic growth factors. Postgraduate medical journal 73, 215-221
连结：
6. Hamilton, J. A. (2008) Colony-stimulating factors in inflammation and autoimmunity. Nature reviews. Immunology 8, 533-544
连结：
7. Savona, M., and Talpaz, M. (2008) Getting to the stem of chronic myeloid leukaemia. Nature reviews. Cancer 8, 341-350
连结：

国际计量

〈TOP〉

E-mail ：

文章公开取用时，将寄通知信至您填写的信箱地址

E-mail ：

使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。