以魚鱗為原料合成氫氧基磷灰石多孔材料應用於生醫領域及重金屬吸附功能之研究

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以鱼鳞为原料合成氢氧基磷灰石多孔材料应用於生医领域及重金属吸附功能之研究

Synthesis of Hydroxyapatite Scaffolds from Fish Scales for Biomedical Engineering and Heavy Metal Ion Removal

刘文光 , 硕士　　导师：陈柏宇　　

英文

鱼鳞；冷冻铸造法；细胞毒性；金属离子吸附； fish scale ； freeze casting ； tissue engineering ； heavy metal removal

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摘要 │ 参考文献 (133) │ 国际计量

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The hydroxyapatite has been extensively investigated with respect to its potential for bone tissue engineering and removal of heavy metal ions from aqueous solutions. Fish scales are a natural source for calcium phosphate source though considered as wastes in daily life. In this study, hydroxyapatite was extracted and obtained from scales of Tilapia fish (Oreochromis mossambicus). The mineral was confirmed to be hydroxyapatite by X-ray diffraction and energy dispersive spectrometry. The hydroxyapatite powders obtained from fish scales was used as raw materials and scaffolds were synthesized by the freeze casting technique. The well aligned laminar structures with 10-80 μm channels was revealed by SEM, meanwhile, the pore size can be controlled by tuning cooling rates. It is able to synthesize scaffolds with microstructure mimicking cancellous bone, which exhibit good mechanical strength. The cell proliferation test was done by culturing human osteoblast cells within the scaffolds, and cell viability was evaluated by the MTS assay. Histological evaluation was carried out to characterize the cell ingrowth. Results confirmed that the scaffolds were not cytotoxic. Additionally, hydroxyapatite has good ability to remove heavy ions in water. We chose lead ion as indicator to test the sorption ability, and used isotherm models to describe the sorption behavior. From Atomic Absorption Spectrometer (AAS) analysis, the scaffolds eliminated 99% of lead ion in a short time, providing efficient capability of heavy metal ions removal. Inexpensive fish scales can be utilized to synthesize hydroxyapatite scaffolds by freeze casting and have great potential to be applied in various fields.

参考文献 ( 133 ) 〈TOP〉

[1] M. A. Meyers and P.-Y. Chen, Biological Materials Science: Biological Materials, Bioinspired Materials, and Biomaterials: Cambridge University Press, 2014.
连结：
[2] P.-Y. Chen, A. Lin, Y.-S. Lin, Y. Seki, A. Stokes, J. Peyras, et al., "Structure and mechanical properties of selected biological materials," Journal of the Mechanical Behavior of Biomedical Materials, vol. 1, pp. 208-226, 2008.
连结：
[3] M. A. Meyers, P.-Y. Chen, A. Y.-M. Lin, and Y. Seki, "Biological materials: Structure and mechanical properties," Progress in Materials Science, vol. 53, pp. 1-206, 2008.
连结：
[4] M. F. Ashby and R. M. Medalist, "The mechanical properties of cellular solids," Metallurgical Transactions A, vol. 14, pp. 1755-1769, 1983.
连结：
[5] U. G. K. Wegst and M. F. Ashby, "The mechanical efficiency of natural materials," Philosophical Magazine, vol. 84, pp. 2167-2186, 2004.
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引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。