Attenuation of Lung Inflammation and Pro-Inflammatory Cytokine Production by Resveratrol Following Trauma-Hemorrhage

Chun-Te Wu；Huang-Ping Yu；Chih-Yang Chung；Ying-Tung Lau；Shuen-Kuei Liao

hemorrhagic shock ； chemokine ； adhesion molecule ； cytokine

The Chinese Journal of Physiology

51卷6期（2008 / 12 / 01）

363 - 368

英文

Although studies have demonstrated that resveratrol administration following adverse circulatory conditions is known to be protective, the mechanism by which resveratrol produces the salutary effects remains unknown. We hypothesized that resveratrol administration in males following traumahemorrhage decreases cytokine production and protects against lung injury. Male Sprague-Dawley rats underwent trauma-hemorrhage (mean blood pressure 40 mmHg for 90 min, then resuscitation). A single dose of resveratrol (30 mg/kg of body weight) or vehicle was administered intravenously during resuscitation. Twenty-four hours thereafter, tissue myeloperoxidase activity (a marker of neutrophil sequestration), cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-3, intercellular adhesion molecule (ICAM)-1, and interleukin (IL)-6 levels in the lung and protein concentrations in bronchoalveolar lavage fluid were measured (n=6 rats/group). One-way ANOVA and Tukey's test were used for statistical analysis. Trauma-hemorrhage increased lung myeloperoxidase activity, CINC-1, CINC-3, ICAM-1, and IL-6 levels and protein concentrations in bronchoalveolar lavage fluid. These parameters were significantly improved in the resveratrol-treated rats subjected to trauma-hemorrhage. The salutary effects of resveratrol administration on attenuation of lung injury following trauma-hemorrhage are likely due to reduction of pro-inflammatory mediators.

1. Wang, G.C.,Chi, W.M.,Perng, W.C.,Huang, K.L.(2003).Body temperature control in sepsis-induced acute lung injury.Chinese J. Physiol.,46,151-157.
   連結：
2. Baur, J.A.,Sinclair, D.A.(2006).Therapeutic potential of resveratrol: the in vivo evidence.Nature,5,493-506.
3. Birrell, M.A.,McCluskie, K.,Wong, S.,Donnelly, L.E.,Barnes, P.J.,Belvisi, M.G.(2005).Resveratrol, an extract of red wine, inhibits lipopolysaccharide induced airway neutrophilia and inflammatory mediators through an NF-κB-independent mechanism.FASEB J.,19,840-841.
4. Dayal, S.D.,Hasko, G.,Lu, Q.,Xu, D.Z.,Caruso, J.M.,Sambol, J.T.,Deitch, E.A.(2002).Trauma/hemorrhagic shock mesenteric lymph upregulates adhesion molecule expression and IL-6 production in human umbilical vein endothelial cells.Shock,17,491-495.
5. Feng, X.,Ren, B.,Xie, W.,Huang, Z.,Liu, J.,Guan, R.,Duan, M.,Xu, J.(2006).Influence of hydroxyethyl starch 130/0.4 in pulmonary neutrophil recruitment and acute lung injury during polymicrobial sepsis in rats.Acta Anaesthesiol. Scand.,50,1081-1088.
6. Ferrero, M.E.,Bertelli, A.A.E.,Fulgenzi, A.,Pellegatta, F.,Corsi, M.M.,Bonfrate, M.,Ferrara, F.,De Caterina, R.,Biovannini, L.,Bertelli, A.(1998).Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium.Am. J. Clin. Nutr.,68,1208-1214.
7. Ferrero, M.E.,Bertelli, A.A.E.,Pellegatta, F.,Fulgenzi, A.,Corsi, M.M.,Bertelli, A.(1998).Phytoalexin resveratrol (3,4`,5`-trihydroxystilbene) modulates granulocyte and monocyte endothelial adhesion.Transplant. Proc.,30,4191-4193.
8. Frink, M.,Lu, A.,Thobe, B.M.,Hsieh, Y.C.,Choudhry, M.A.,Schwacha, M.G.,Kunkel, S.L.,Chaudry, I.H.(2007).Monocyte chemoattractant protein-1 influences trauma-hemorrhage-induced distal organ damage via regulation of keratinocyte-derived chemokine production.Am. J. Physiol. Regul. Integr. Comp. Physiol.,292,1110-1116.
9. Gao, X.,Xu, Y.X.,Janakiraman, N.,Chapman, R.A.,Gautam, S.C.(2001).Immunomodulatory activity of resveratrol: suppression of lymphocyte proliferation, development of cell-mediated cytotoxicity, and cytokine production.Biochem. Pharmacol.,62,1299-1308.
10. Guo, R.F.,Riedemann, N.C.,Laudes, I.J.,Sarma, V.J.,Kunkel, R. G.,Dilley, K.A.,Paulauskis, J.D.,Ward, P.A.(2002).Altered neutrophil trafficking during sepsis.J. Immunol.,169,307-314.
11. Iida, M.,Watanabe, K.,Tsurufuji, M.,Takaishi, K.,Iizuka, Y.,Tsurufuji, S.(1992).Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats.Infect. Immun.,60,1268-1272.
12. Khadaroo, R.G.,Fan, J.,Power, K.A.,Fann, B.,Kapus, A.,Rotstein, O.D.(2004).Impaired induction of IL-10 expression in the lung following hemorrhagic shock.Shock,22,333-339.
13. Kira, S.,Daa, T.,Kashima, K.,Mori, M.,Noguchi, T.,Yokoyama, S.(2005).Mild hypothermia reduces expression of intercellular adhesion molecule-1 (ICAM-1) and the accumulation of neutrophils after acid-induced lung in the rat.Acta Anaesthesiol. Scand.,49,351-359.
14. Maier, M.,Strobele, H.,Voges, J.,Bauer, C.,Marzi, I.(2003).Attenuation of leukocyte adhesion by recombinant TNF-binding protein after hemorrhagic shock in the rat.Shock,19,457-461.
15. Malech, H.,Gallin, J.I.(1987).Current concepts: immunology. Neutrophils in human diseases.N. Engl. J. Med.,317,687-694.
16. Manna, S.K.,Mukhopadhyay, A.,Aggarwal, B.B.(2000).Resveratrol suppresses TNF-induced activation of nuclear transcription factor NF-κB, activator protein-1, and apoptosis: potential role of reactive oxygen intermediates and lipid peroxidation.J. Immunol.,164,6509-6519.
17. Meldrum, D.R.,Meng, X.,Sheridan, B.C.,McIntyre, R.C.,Harken, A.H.,Banerjee, A.(1998).Tissue-specific protein kinase C isoforms differentially mediate macrophage TNFα and IL-1β production.Shock,9,256-260.
18. Meng, Z.H.,Dyer, K.,Billiar, T.R.,Tweardy, D.J.(2001).Essential role for IL-6 in postresuscitation inflammation in hemorrhagic shock.Am. J. Physiol. Cell Physiol.,280,343-351.
19. Pendurthi, U.R.,Rao, V.M.(2002).Resveratrol suppresses agonist-induced monocyte adhesion to cultured human endothelial cells.Throm. Res.,106,243-248.
20. Sato, M.,Maulik, N.,Das, D.K.(2002).Cardioprotection with alcohol: role of both alcohol and polyphenolic antioxidants.Ann. N. Y. Acad. Sci.,957,122-135.
21. Shanley, T.P.,Schmal, H.,Warner, R.L.,Schmid, E.,Friedl, H.P.,Ward, P.A.(1997).Requirement for C-X-C chemokines (macrophage inflammatory protein-2 and cytokine-induced neutrophil chemoattractant) in IgG immune complex-induced lung injury.J. Immunol.,158,3439-3448.
22. von Andrian, U.H.,Chambers, J.D.,McEvoy, L.M.,Barqatze, R.F.,Arfors, K.E.,Butcher, E.C.(1991).Two-step model of leukocyteendothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo.Proc. Natl. Acad. Sci. USA,88,7538-7542.
23. Wang, P.,Ba, Z.F.,Lu, M.C.,Ayala, A.,Harkema, J.M.,Chaudry, I.H.(1994).Measurement of circulating blood volume in vivo after trauma-hemorrhage and hemodilution.Am. J. Physiol.,266,368-374.
24. Wickel, D.,Mercer-Jones, M.A.,Cheadle, W.G.,Mercer-Jones, M.A.,Garrison, R.N.(1997).Poor outcome from peritonitis is caused by disease acuity and organ failure, not recurrent peritoneal infection.Ann. Surg.,225,744-756.
25. Yu, H.P.,Hsieh, Y.C.,Suzuki, T.,Shimizu, T.,Choudhry, M.A.,Schwacha, M.G.,Chaudry, I.H.(2006).Salutary effects of estrogen receptor-β agonist on lung injury after trauma-hemorrhage.Am. J. Physiol. Lung Cell. Mol. Physiol.,290,1004-1009.
26. Yu, H.P.,Shimizu, T.,Hsieh, Y.C.,Suzuki, T.,Choudhry, M.A.,Schwacha, M.G.,Chaudry, I.H.(2006).Tissue specific expression and their role in the regulation of neutrophil infiltration in various organs following trauma-hemorrhage.J. Leukoc. Biol.,79,963-970.
27. Yu, H.P.,Yang, S.,Choudhry, M.A.,Hsieh, Y.C.,Bland, K.I.,Chaudry, I.H.(2005).Mechanism responsible for the salutary effects of flutamide on cardiac performance following trauma-hemorrhagic shock: Upregulation of cardiomyocyte estrogen receptors.Surgery,138,85-92.
28. Yu, H.P.,Yang, S.,Hsieh, Y.C.,Choudhry, M.A.,Bland, K.I.,Chaudry, I.H.(2006).Maintenance of lung myeloperoxidase activity in proestrus females after trauma-hemorrhage: upregulation of heme oxygenase-1.Am. J. Physiol. Lung Cell. Mol. Physiol.,291,400-406.
29. Zhong, M.,Cheng, G.F.,Wang, W.J.,Guo, Y.,Zhu, X.Y.,Zhang, J.T.(1999).Inhibitory effect of resveratrol on interleukin-6 release by stimulated peritoneal macrophages of mice.Phytomdicine,6,79-84.

1. Wang, Shyi-Wu,Su, Mao-Kuan,Shiang, Tzyy-Yuang,Lee, Shu-Lin,Hsu, Mei-Chich,Chu, Po-Ju,Chen, Szu-Tah,Chen, Kenny Wen-Chyuan,Chen, Chuan-Show,Chang, Ying-Ling,Chang, Mu-San(2011).Effect of Passive Repetitive Isokinetic Training on Cytokines and Hormonal Changes.中國生理學雜誌,54(1),55-66.
2. Yu, Huang-Ping,Liu, Fu-Chao,Li, Allen H.,Lau, Ying-Tung,Huang, Yi-Shun(2011).Astringinin-Mediated Attenuation of the Hepatic Injury following Trauma-Hemorrhage.中國生理學雜誌,54(3),183-189.