運動後氧氣供應對肝醣回補之影響

Effect of Oxygen Supply on Glycogen Recovery and AMPK Protein Expression in Post-Exercise

10.5297/ser.1404.009

李文志(Wen-Chih Lee)；張惟翔(Wei-Hsiang Chang)；侯建文(Chien-Wen Hou)；徐欣億(Hsin-Yi Hsu)

運動恢復期 ； 常壓高氧 ； 常壓低氧 ； oxygen concentration recovery ； normobaric hyperoxia ； normobaric hypoxia

大專體育學刊

14卷4期（2012 / 12 / 31）

483 - 491

繁體中文;英文

本研究目的是以動物模式探討運動後不同氧氣濃度下恢復1小時，對不同組織肝醣回補情形與肌肉AMPK蛋白質表現之影響。研究方法是以48隻Sprague Dawley 鼠（體重約250公克）為試驗對象，試驗設計分成無運動常氧組、無運動低氧組、無運動高氧組、運動常氧組、運動低氧組、運動高氧組等6組。運動組老鼠在水溫34℃下，連續游泳運動3小時後，於高氧（FIO2 = 83%）、常氧（FIO2 = 21%）及低氧（FIO2 =14%）等不同氧氣濃度下恢復1小時。運動及恢復後測量血糖及胰島素，犧牲後分析老鼠心肌、肝臟、股四頭紅肌、白肌的肝醣含量、AMPK及磷酸化AMPK蛋白質表現量。研究結果顯示在恢復1小時後運動組血糖恢復至與無運動組相同，恢復後胰島素各組之間無差異。不同氧氣濃度恢復對各組織肝醣的影響，運動組心肌肝醣明顯較無運動組高，肝臟肝醣則是各組無差異。股四頭白肌之肝醣，運動組明顯較無運動組低，而運動組中低氧組之肝醣明顯高於常氧組。股四頭紅肌之肝醣，高氧運動組明顯較無運動組低，而運動低氧組明顯高於運動高氧組。總AMPK表現量在白肌中，各組無明顯差異，磷酸化AMPK在白肌中，則是運動高氧組明顯較高於運動低氧組。研究結論是運動恢復期各組織中，以心肌肝醣回補最快，而低氧在運動恢復期對肝醣回補效果較佳，而AMPK路徑不是影響低氧肝醣回補的關鍵。

The purpose of this study was to investigate the glycogen restoration and AMPK protein expression in post-exercised rats under recovery with different oxygen concentrations. Forty-eight Sprague Dawley rats (weighing 250 grams) were divided into exercise and non-exercise groups (control) and recovered under normobaric, normobaric-hypoxia (FIO2 = 14%) and normobaric-hyperoxia (FIO2 = 83%) conditions. In exercise groups rats were allowed to swim for 3-h continuously, and then recovered for 1-h under three different oxygen concentrations. After recovery the blood glucose levels were not significantly altered between exercise and non-exercise groups. Insulin levels were similar with blood glucose levels during recovery. The glycogen content in cardiac muscle was significantly increased in exercise groups compared to non-exercise groups after recovery, but no difference in liver glycogen between exercise and non-exercise groups. On the other hand, white quadriceps glycogen content was significantly decreased in exercise group, but restoration of glycogen under hypoxia recovery was higher than hyperoxia recovery in exercise group. Interestingly, the glycogen restoration in red quadriceps was similar with white quadriceps. The total AMPK protein expression was no difference between exercise and non-exercise groups; however, the phosphorylated AMPK protein was higher in hyperoxia than hypoxia in white quadriceps. Our findings conclude that the restoration of glycogen in cardiac muscle was taken into first priority than the other tissues. Furthermore, normobaric hypoxia condition would be helpful for the glycogen restoration in muscle after exercise, and AMPK signal pathway may not be the key factor for glycogen restoration in rats.

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