轉殖小枯草桿菌纖維素酶前處理纖維對磨漿特性的影響

楊千瑩

doi:10.6342/NTU.2011.01220

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转殖小枯草杆菌纤维素酶前处理纤维对磨浆特性的影响

Impacts of Recombinant Paenibacillus Cellulase Pretreatment on Pulp Refining Characteristics

杨千莹 , 硕士　　导师：柯淳涵　　

英文 DOI： 10.6342/NTU.2011.01220

纸浆；酵素；磨浆；纸力性质；滤水性； PFI磨浆机；电子扫描显微镜；原子力学扫描显微镜； pulp ； enzyme ； refining ； paper property ； drainability ； PFI ； SEM ； AFM

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摘要 │ 参考文献 (48) │ 国际计量

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This study aimed at the progress of application of cellulase in refining, including mechanism of action on cellulose and their effects on physical properties on paper. Utilization of enzymes becomes a popular biotechnology, and there are more and more researches confirm their potential. Refining is a necessary process to form the paper where the cellulosic fibers are mechanically treated, resulting into morphological and structural changes to produce desired fiber properties for a better quality paper. 
However, refining is energy-cost stage in pulp and paper industry. For this reason, the effect of cellulase was evaluated for energy saving in refining process of papermaking. The recombinant Paenibacillus cellulase was used to improve the efficiency of the refining process of two pulps, using different enzyme dosages and different PFI revolutions. The pulps used in this work were the commercial bleached Eucalyptus globulus kraft pulp and Pseudotsuga menziesii kraft pulp. 
This treatment increased drainability of both pulps at the same level of refining energy. In other words, that consumed less refining revolutions to reach the same drainability compared to the untreated pulps. Although the viscosity of both pulps were degrade with the enzymatic treatment, the physical properties of paper had no significant negatively influence on them. Moreover, the samples were observed by Scanning electron microscopy (SEM), and analyzed the roughness and height of fiber by Atomic force microscopy (AFM).　The results showed the treatment with cellulase swelled the fibers in the absence of refining, and there were better fibrillation on the fibers treated with cellulase after refining. Furthermore, the statistical analysis of AFM suggested the both pulps treated with low cellulase dosage with PFI refining had higher roughness.

参考文献 ( 48 ) 〈TOP〉

Bajpai, P., Bajpai, P., International, P. 1996b. Organochlorine Compounds in Bleach Plant Effluents: Genesis and Control. Pira International Surrey, UK.
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Bajpai, P. 1997. Microbial xylanolytic enzyme system: properties and applications. Advances in Applied Microbiology, 43, 141-194.
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Bajpai, P. 1999. Application of enzymes in the pulp and paper industry. Biotechnology Progress, 15(2), 147-157.
连结：
Bhardwaj, N., Bajpai, P., Bajpai, P. 1996. Use of enzymes in modification of fibres for improved beatability. Journal of Biotechnology, 51(1), 21-26.
连结：
Bhardwaj, N., Hoang, V., Nguyen, K. 2007. Effect of refining on pulp surface charge accessible to polydadmac and FTIR characteristic bands of high yield kraft fibres. Bioresource Technology, 98(4), 962-966.
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引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。