探討脂肪幹細胞以及BALB/c 3T3纖維母細胞在蠶絲布上的生長特性

林俐岑

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探讨脂肪干细胞以及BALB/c 3T3纤维母细胞在蚕丝布上的生长特性

Investigation of the properties of adipose-derived stem cells and BALB/c 3T3 fibroblasts grown on silk fabric-based scaffolds

林俐岑 , 硕士　　导师：张晃猷　　

英文

疝气；蚕丝蛋白；脂肪干细胞；纤维母细胞；蚕丝布；胶原蛋白； hernia ； fibroin ； adipose-derived stem cell ； fibroblast ； silk fabric ； collagen

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摘要 │ 参考文献 (58) │ 国际计量

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A hernia is a common disease occurs when an organ protrudes through a tissue holding it in the normal position. Surgeons use meshes to repair hernias. Most of the meshes are made of synthetic materials such as polyethylene, polypropylene, polyester and polytetrafluoroethylene that may easily lead to foreign body reactions. Silk fibroin from silkworm is a nature polymer applied widely in tissue engineering, possessing excellent mechanical property and biocompatibility. We proposed that silk fibroin has a potential to be used as a hernia repair mesh when knitted into a fabric. This study utilized silk fabric, obtained from a commercial source, as a scaffold to support cell growth. We investigated the characteristics of cells grown on the scaffold and determined whether the scaffold evokes an immune response. First, BALB/c 3T3 and adipose-derived stem cells (ADSCs) cultured on a silk fabric with or without dissolved fibroin or collagen coating were examined by LIVE/DEAD staining. Cell viability of BALB/c 3T3 and ADSCs was measured using alamarBlue assay. Results showed that silk fabric with fibroin coating improved BALB/c 3T3 and ADSCs growth. Furthermore, we found that basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) were produced in a higher level from ADSCs cultured on silk fabric with fibroin coating. Next, we tested TNF and IL-6 production from macrophage line RAW264.7 cultured on silk fabric. The result showed that silk fabric would not trigger TNF and IL-6 production from RAW264.7. Furthermore, silk fabric would not degrade in culture medium for several days. Therefore, silk fabric with fibroin or collagen coating can be developed into a novel hernia repair mesh in the future. To sum up, silk fibroin and collagen provide a suitable matrix for cell growth on silk fabric.

参考文献 ( 58 ) 〈TOP〉

2. Sharma, A. and P. Chelawat, Endo-laparoscopic inguinal hernia repair: What is its role? Asian J Endosc Surg, 2017 May;10(2):111-118.
连结：
3. Raveenthiran, V. and Agarwal P, Choice of Repairing Inguinal Hernia in Children: Open Versus Laparoscopy. Indian J Pediatr, 2017 May 27.
连结：
4. Garcia-Amador C, De la Plaza R, Arteaga V, Lopez-Marcano A, Ramia J, Garengeot's hernia: two case reports with CT diagnosis and literature review. Open Med (Wars), 2016 Oct 7;11(1):354-360.
连结：
5. Brown, C.N. and J.G. Finch, Which mesh for hernia repair? Ann R Coll Surg Engl, 2010. 92(4): 272-8.
连结：
6. Smart NJ, Bryan N, Hunt J A, Daniels IR. Porcine dermis implants in soft-tissue reconstruction: current status. Biologics, 2014 Mar 10;8:83-90.
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使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。